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GAD1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6300943

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
GAD1, Polyclonal Antibody
Full Product Name
GAD1, CT (GAD1, GAD, GAD67, Glutamate decarboxylase 1, 67kD glutamic acid decarboxylase, Glutamate decarboxylase 67kD isoform) (Azide free) (HRP)
Product Gene Name
anti-GAD1 antibody
Product Synonym Names
GAD1; GAD; GAD67; Glutamate decarboxylase 1; 67kD glutamic acid decarboxylase; Glutamate decarboxylase 67kD isoform
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
69.3 kDa (617aa)
OMIM
603513
3D Structure
ModBase 3D Structure for Q99259
Immunogen
GAD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 521-551 amino acids from the C-terminal region of human GAD1.
Conjugate
HRP
Note
Preservative Free

Product Categories/Family for anti-GAD1 antibody

Antibodies; Enzymes; Decarboxylase
Applications Tested/Suitable for anti-GAD1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-GAD1 antibody
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-GAD1 antibody

GAD1 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the GAD1 antibody detected the GAD1 protein (arrow).
Rabbit Anti-GAD1 Polyclonal Antibody – Cat# MBS6300943

Product References and Citations for anti-GAD1 antibody

Lanoue, A.C., et al. Exp. Neurol. 226(1):207-217(2010). Jia, P., et al. Schizophr. Res. 122 (1-3), 38-42 (2010): Terranova, C., et al. Alcohol 44(5):407-413(2010). Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010). Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010).

Other Notes

Small volumes of anti-GAD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GAD1 purchase

MBS6300943 is an antibody targeting GAD1. Also known as GAD1, CT (GAD1, GAD, GAD67, Glutamate decarboxylase 1, 67kD glutamic acid decarboxylase, Glutamate decarboxylase 67kD isoform) (Azide free) (HRP). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GAD1. It may not necessarily be applicable to this product.

NCBI GI #
58331246
NCBI GeneID
2571
NCBI Accession #
NP_000808
UniProt Primary Accession #
Q99259
UniProt Secondary Accession #
Q49AK1; Q53TQ7; Q9BU91; Q9UHH4
UniProt Related Accession #
Q99259
GenBank Nucleotide #
NM_000817.2
NCBI Official Full Name
glutamate decarboxylase 1 isoform GAD67
NCBI Official Synonym Full Names
glutamate decarboxylase 1
NCBI Official Symbol
GAD1
NCBI Official Synonym Symbols
GAD; SCP; CPSQ1
UniProt Protein Name
Glutamate decarboxylase 1
UniProt Synonym Protein Names
67 kDa glutamic acid decarboxylase; GAD-67; Glutamate decarboxylase 67 kDa isoform
Protein Family
Glutamate decarboxylase
UniProt Gene Name
GAD1
UniProt Synonym Gene Names
GAD; GAD67; GAD-67
UniProt Entry Name
DCE1_HUMAN
NCBI Protein Information
glutamate decarboxylase 1

UniProt Comments for GAD1

GAD67: Catalyzes the production of GABA. Homodimer. Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. Belongs to the group II decarboxylase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Other Amino Acids Metabolism - taurine and hypotaurine; Other Amino Acids Metabolism - beta-alanine; Carbohydrate Metabolism - butanoate; Lyase; EC 4.1.1.15; Mitochondrial

Chromosomal Location of Human Ortholog: 2q31

Cellular Component: plasma membrane; intracellular; vesicle membrane

Molecular Function: protein binding; glutamate binding; glutamate decarboxylase activity; protein heterodimerization activity; protein N-terminus binding; pyridoxal phosphate binding

Biological Process: response to drug; glutamate catabolic process; synaptic transmission; glutamate decarboxylation to succinate; neurotransmitter secretion; gamma-aminobutyric acid biosynthetic process; protein-pyridoxal-5-phosphate linkage; neurotransmitter biosynthetic process

Disease: Cerebral Palsy, Spastic Quadriplegic, 1
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Research Articles on GAD1

1. findings converge on variation in glutamic acid decarboxylase 1 gene predicting a susceptibility mechanism that affects white matter fractional anisotropy, GABAergic inhibitory neurotransmission in the dorsolateral prefrontal cortex, and working memory performance.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GAD1 antibody

 Reference Product  PubMed Publications
 GAD2 antibody  >40 publications with GAD1 and GAD2

Pathways associated with anti-GAD1 antibody

 Products by Pathway  Pathway Diagram
 Alanine And Aspartate Metabolism Pathway antibodies  Alanine And Aspartate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Biogenic Amine Synthesis Pathway antibodies  Biogenic Amine Synthesis Pathway Diagram
 Butanoate Metabolism Pathway antibodies  Butanoate Metabolism Pathway Diagram
 Butanoate Metabolism Pathway antibodies  Butanoate Metabolism Pathway Diagram
 GABA (gamma-Aminobutyrate) Shunt Pathway antibodies  GABA (gamma-Aminobutyrate) Shunt Pathway Diagram
 GABA (gamma-Aminobutyrate) Shunt Pathway antibodies  GABA (gamma-Aminobutyrate) Shunt Pathway Diagram
 GABA Synthesis Pathway antibodies  GABA Synthesis Pathway Diagram
 GABA Synthesis, Release, Reuptake And Degradation Pathway antibodies  GABA Synthesis, Release, Reuptake And Degradation Pathway Diagram

Diseases associated with anti-GAD1 antibody

 Disease Name  Pubmed Publications
 Mental Disorders Antibodies  >47 publications with GAD1 and Mental Disorders
 Schizophrenia Antibodies  >30 publications with GAD1 and Schizophrenia
 Nervous System Diseases Antibodies  >23 publications with GAD1 and Nervous System Diseases
 Brain Diseases Antibodies  >18 publications with GAD1 and Brain Diseases
 Bipolar Disorder Antibodies  >13 publications with GAD1 and Bipolar Disorder
 Disease Models, Animal Antibodies  >11 publications with GAD1 and Disease Models, Animal
 Seizures Antibodies  >9 publications with GAD1 and Seizures
 Autistic Disorder Antibodies  >5 publications with GAD1 and Autistic Disorder
 Anxiety Disorders Antibodies  >5 publications with GAD1 and Anxiety Disorders
 Depressive Disorder Antibodies  >5 publications with GAD1 and Depressive Disorder

Organs/Tissues associated with anti-GAD1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >78 publications with GAD1 and Brain
 Muscle Antibodies  >2 publications with GAD1 and Muscle
 Kidney Antibodies  >2 publications with GAD1 and Kidney
 Bone Antibodies  >1 publications with GAD1 and Bone
 Embryonic Tissue Antibodies  >1 publications with GAD1 and Embryonic Tissue
 Intestine Antibodies  >1 publications with GAD1 and Intestine
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