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GAREM1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1759287

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
GAREM1, Polyclonal Antibody
Full Product Name
Anti-GAREM1 Antibody Picoband
Product Gene Name
anti-GAREM1 antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
Molecular Weight
97,099 Da
3D Structure
ModBase 3D Structure for Q9H706
Immunogen
E coli-derived human GAREM1 recombinant protein (Position: Q277-E753). Human GAREM1 shares 86.8% amino acid (aa) sequence identity with mouse GAREM1.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.

Product Categories/Family for anti-GAREM1 antibody

Primary Antibodies
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-GAREM1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-GAREM1 antibody
WB: 0.25-0.5ug/ml, Human
IHC: 2-5ug/ml, Human
FC/FACS (Fixed): 1-3ug/1x106 cells, Human
ELISA: 0.1-0.5ug/ml

Western Blot (WB) of anti-GAREM1 antibody

Figure 1. Western blot analysis of GAREM1 using anti-GAREM1 antibody (MBS1759287).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human RT4 whole cell lysates,
Lane 3: human Hacat whole cell lysates,
Lane 4: human SIHA whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAREM1 antigen affinity purified polyclonal antibody (#MBS1759287) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for GAREM1 at approximately 97 kDa. The expected band size for GAREM1 is at 97 kDa.
Rabbit Anti-GAREM1 Polyclonal Antibody – Cat# MBS1759287

Immunohistochemistry (IHC) of anti-GAREM1 antibody

Figure 2. IHC analysis of GAREM1 using anti-GAREM1 antibody (MBS1759287).
GAREM1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GAREM1 Antibody (MBS1759287) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (#MBS176453) with DAB as the chromogen.
Rabbit Anti-GAREM1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1759287

Immunohistochemistry (IHC) of anti-GAREM1 antibody

Figure 3. IHC analysis of GAREM1 using anti-GAREM1 antibody (MBS1759287).
GAREM1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GAREM1 Antibody (MBS1759287) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (#MBS176453) with DAB as the chromogen.
Rabbit Anti-GAREM1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1759287

Flow Cytometry (FC/FACS) of anti-GAREM1 antibody

Figure 4. Flow Cytometry analysis of SH-SY5Y cells using anti-GAREM1 antibody (MBS1759287).
Overlay histogram showing SH-SY5Y cells stained with MBS17592871 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-GAREM1 Antibody (MBS1759287, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (MBS177405, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Rabbit Anti-GAREM1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1759287

Product References and Citations for anti-GAREM1 antibody

1. Nishino, T., Matsunaga, R., Konishi, H. Functional relationship between CABIT, SAM and 14-3-3 binding domains of GAREM1 that play a role in its subcellular localization. Biochem. Biophys. Res. Commun. 464: 616-621, 2015. 2. Taniguchi, T., Tanaka, S., Ishii, A., Watanabe, M., Fujitani, N., Sugeo, A., Gotoh, S., Ohta, T., Hiyoshi, M., Matsuzaki, H., Sakai, N., Konishi, H. A brain-specific Grb2-associated regulator of extracellular signal-regulated kinase (Erk)mitogen-activated protein kinase (MAPK)(GAREM) subtype, GAREM2, contributes to neurite outgrowth of neuroblastoma cells by regulating Erk signaling. J. Biol. Chem. 288: 29934-29942, 2013. 3. Tashiro, K., Tsunematsu, T., Okubo, H., Ohta, T., Sano, E., Yamauchi, E., Taniguchi, H., Konishi, H. GAREM, a novel adaptor protein for growth factor receptor-bound protein 2, contributes to cellular transformation through activation of extracellular signal-regulated kinase signaling. J. Biol. Chem. 284: 20206-20214, 2009.

Other Notes

Small volumes of anti-GAREM1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GAREM1 purchase

MBS1759287 is an antibody targeting GAREM1. Also known as Anti-GAREM1 Antibody Picoband. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GAREM1. It may not necessarily be applicable to this product.

NCBI GI #
125991851
NCBI GeneID
64762
NCBI Accession #
Q9H706.2
UniProt Primary Accession #
Q9H706
UniProt Secondary Accession #
Q0VAG3; Q0VAG4; Q8ND03; Q9BSF5
UniProt Related Accession #
Q9H706
NCBI Official Full Name
GRB2-associated and regulator of MAPK protein
NCBI Official Synonym Full Names
GRB2 associated, regulator of MAPK1
NCBI Official Symbol
GAREM
NCBI Official Synonym Symbols
Gm944; FAM59A; C18orf11
UniProt Protein Name
GRB2-associated and regulator of MAPK protein
UniProt Synonym Protein Names
GRB2-associated and regulator of MAPK1
Protein Family
GRB2-associated and regulator of MAPK protein
UniProt Gene Name
GAREM
UniProt Synonym Gene Names
C18orf11; FAM59A
UniProt Entry Name
GAREM_HUMAN
NCBI Protein Information
GRB2-associated and regulator of MAPK protein; protein FAM59A; GRB2-associated and regulator of MAPK1; Grb2-associated and regulator of Erk/MAPK; family with sequence similarity 59, member A

NCBI Summary for GAREM1

This gene encodes an adaptor protein which functions in the epidermal growth factor (EGF) receptor-mediated signaling pathway. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

UniProt Comments for GAREM1

GAREM: a protein of unknown function. Contains a predicted ATPase (AAA+ superfamily).

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 18q12.1

Cellular Component: plasma membrane

Molecular Function: protein binding

Biological Process: epidermal growth factor receptor signaling pathway; positive regulation of cell division; positive regulation of cell proliferation
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Research Articles on GAREM1

1. Data indicate that a subtype of GAREM, GAREM2, is specifically expressed in the mouse, rat, and human brain.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GAREM1 antibody

 Reference Product  PubMed Publications
 GRB2 antibody  >3 publications with GAREM1 and GRB2

Diseases associated with anti-GAREM1 antibody

 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >11 publications with GAREM1 and Liver Diseases
 Lung Diseases Antibodies  >4 publications with GAREM1 and Lung Diseases
 Atrophy Antibodies  >3 publications with GAREM1 and Atrophy
 Fibrosis Antibodies  >3 publications with GAREM1 and Fibrosis
 Disease Models, Animal Antibodies  >2 publications with GAREM1 and Disease Models, Animal
 Anemia Antibodies  >2 publications with GAREM1 and Anemia
 Kidney Diseases Antibodies  >1 publications with GAREM1 and Kidney Diseases
 Pain Antibodies  >1 publications with GAREM1 and Pain
 Hepatomegaly Antibodies  >1 publications with GAREM1 and Hepatomegaly
 Necrosis Antibodies  >1 publications with GAREM1 and Necrosis

Organs/Tissues associated with anti-GAREM1 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >16 publications with GAREM1 and Liver
 Blood Antibodies  >11 publications with GAREM1 and Blood
 Nerve Antibodies  >10 publications with GAREM1 and Nerve
 Bone Antibodies  >8 publications with GAREM1 and Bone
 Lung Antibodies  >7 publications with GAREM1 and Lung
 Skin Antibodies  >6 publications with GAREM1 and Skin
 Brain Antibodies  >5 publications with GAREM1 and Brain
 Muscle Antibodies  >4 publications with GAREM1 and Muscle
 Mouth Antibodies  >4 publications with GAREM1 and Mouth
 Intestine Antibodies  >3 publications with GAREM1 and Intestine
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