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GM2A Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8106875

GM2A (GM2 ganglioside activator), is a lipid transfer protein which belongs to the ML domain family. GM2A can accommodate several single chain phospholipids and fatty acids. It also exhibits some calcium-independent phospholipase activity. GM2A binds… More details

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Product Overview

Online Datasheet in PDF
Product Name
GM2A, Polyclonal Antibody
Full Product Name
Anti-GM2A Antibody, Rabbit Polyclonal
Product Gene Name
anti-GM2A antibody
Product Synonym Names
GM2A Antibody, Rabbit PAb, Antigen Affinity Purified; GM2 ganglioside activator; Anti-GM2-AP Antibody; Anti-SAP-3 Antibody
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid; 0.2um filtered solution in PBS
Purity/Purification
Protein A & Antigen Affinity
Specificity
Human GM2A
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Molecular Weight
20,838 Da
Immunogen
Recombinant Human GM2A Protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human GM2A (rh GM2A). GM2A specific IgG was purified by Human GM2A affinity chromatography.
Applications Tested/Suitable for anti-GM2A antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
Application Notes for anti-GM2A antibody
WB: 1:500-1:2000
ELISA: 1:5000-1:10,000
IHC-P: 1:500-1:2000
IP: 5-10uL/mg of lysate

Western Blot (WB) of anti-GM2A antibody

Anti-GM2A rabbit polyclonal antibody at 1:500 dilution
Lane A: HeLa Whole Cell Lysate
Lysates/proteins at 30 ug per lane.
Secondary
Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:21 kDa
Observed band size:21 kDa
(We are unsure as to the identity of these extra bands.)
Rabbit Anti-GM2A Polyclonal Antibody – Cat# MBS8106875

Immunohistochemistry (IHC) of anti-GM2A antibody

Immunochemical staining of human GM2A in human kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
Rabbit Anti-GM2A Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS8106875

Immunohistochemistry (IHC) of anti-GM2A antibody

Immunochemical staining of human GM2A in human skin with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
Rabbit Anti-GM2A Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS8106875

Immunoprecipitation (IP) of anti-GM2A antibody

GM2A was immunoprecipitated using:
Lane A:0.5 mg HeLa Whole Cell Lysate
4 uL anti-GM2A rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.
Primary antibody:
Anti-GM2A rabbit polyclonal antibody,at 1:100 dilution
Secondary antibody:
Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size :21 kDa
Rabbit Anti-GM2A Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS8106875

Product References and Citations for anti-GM2A antibody

Wright CS, et al. (2003) Structural analysis of lipid complexes of GM2-activator protein. J Mol Biol. 331(4):951-64.

Other Notes

Small volumes of anti-GM2A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GM2A purchase

MBS8106875 is an antibody targeting GM2A. Also known as Anti-GM2A Antibody, Rabbit Polyclonal. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GM2A. It may not necessarily be applicable to this product.

NCBI GI #
183357
NCBI GeneID
2760
NCBI Accession #
AAA35907.1
UniProt Secondary Accession #
Q14426; Q14428; Q6LBL5; B2R699; D3DQH6
UniProt Related Accession #
P17900
NCBI Official Full Name
G-M2 activator protein
NCBI Official Synonym Full Names
GM2 ganglioside activator
NCBI Official Symbol
GM2A
NCBI Official Synonym Symbols
SAP-3; GM2-AP
Protein Family
Ganglioside GM2 activator
NCBI Protein Information
ganglioside GM2 activator

NCBI Summary for GM2A

This gene encodes a small glycolipid transport protein which acts as a substrate specific co-factor for the lysosomal enzyme beta-hexosaminidase A. Beta-hexosaminidase A, together with GM2 ganglioside activator, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Mutations in this gene result in GM2-gangliosidosis type AB or the AB variant of Tay-Sachs disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2009]
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Research Articles on GM2A

1. Studies indicate that sphingolipid activator proteins (SAPs) and anionic lipids are essential stimulators to reach physiological rates of lysosomal sphingolipid degradation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-GM2A antibody

 Products by Pathway  Pathway Diagram
 Glycosphingolipid Metabolism Pathway antibodies  Glycosphingolipid Metabolism Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Neutrophil Degranulation Pathway antibodies  Neutrophil Degranulation Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram
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