• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

GZMB Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9209305

Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides… More details

$240 / 0.08 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GZMB, Polyclonal Antibody
Full Product Name
GZMB Antibody (C-term)
Product Gene Name
anti-GZMB antibody
Product Synonym Names
Granzyme B; C11; CTLA-1; Cathepsin G-like 1; CTSGL1; Cytotoxic T-lymphocyte proteinase 2; Lymphocyte protease; Fragmentin-2; Granzyme-2; Human lymphocyte protein; HLP; SECT; T-cell serine protease 1-3E; GZMB; CGL1; CSPB; CTLA1; GRB
Clonality
Polyclonal
Isotype
Rabbit Ig
Host
Rabbit
Species Reactivity
Human
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Concentration
Vial Concentration: 2 (lot specific)
Specificity
This GZMB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 175-203 amino acids from the C-terminal region of human GZMB.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
GZMB peptide (MBS9226170) is used for blocking the activity of GZMB antibody (MBS9209305)
Molecular Weight
27716
Immunogen Sequence Positions
175-203
OMIM
123910
3D Structure
ModBase 3D Structure for P10144
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN

Product Categories/Family for anti-GZMB antibody

Cancer; Cell Biology; Immunology; Signal Transduction
Applications Tested/Suitable for anti-GZMB antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-GZMB antibody
WB~~1:1000

Western Blot (WB) of anti-GZMB antibody

Western blot analysis of GZMB Antibody (C-term) in HL-60 cell line lysates (35ug/lane). GZMB (arrow) was detected using the purified Pab.
Rabbit Anti-GZMB Polyclonal Antibody – Cat# MBS9209305

Immunohistochemistry (IHC) of anti-GZMB antibody

GZMB Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human Lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GZMB Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-GZMB Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9209305

Flow Cytometry (FC/FACS) of anti-GZMB antibody

GZMB Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-GZMB Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS9209305

Western Blot (WB) of anti-GZMB antibody

Western blot analysis of GZMB (arrow) using rabbit polyclonal GZMB Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GZMB gene.
Rabbit Anti-GZMB Polyclonal Antibody, Western Blot (WB) – Cat# MBS9209305

Product References and Citations for anti-GZMB antibody

Hagn,M., J. Immunol. 183 (3), 1838-1845 (2009)
Gaafar,A., Exp. Hematol. 37 (7), 838-848 (2009)
Girnita,D.M., Transplantation 87 (12), 1801-1806 (2009)

Other Notes

Small volumes of anti-GZMB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GZMB purchase

MBS9209305 is an antibody targeting GZMB. Also known as GZMB Antibody (C-term). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GZMB. It may not necessarily be applicable to this product.

NCBI GI #
221625528
NCBI GeneID
3002
NCBI Accession #
NP_004122.2
UniProt Primary Accession #
P10144
UniProt Secondary Accession #
Q8N1D2; Q9UCC1
UniProt Related Accession #
P10144
GenBank Nucleotide #
NM_004131.4
NCBI Official Full Name
granzyme B
NCBI Official Synonym Full Names
granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
NCBI Official Symbol
GZMB
NCBI Official Synonym Symbols
HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1
UniProt Protein Name
Granzyme B
UniProt Synonym Protein Names
C11; CTLA-1; Cathepsin G-like 1; CTSGL1; Cytotoxic T-lymphocyte proteinase 2; Lymphocyte protease; Fragmentin-2; Granzyme-2; Human lymphocyte protein; HLP; SECT; T-cell serine protease 1-3E
Protein Family
Granzyme
UniProt Gene Name
GZMB
UniProt Synonym Gene Names
CGL1; CSPB; CTLA1; GRB; CTSGL1; Lymphocyte protease; HLP
UniProt Entry Name
GRAB_HUMAN
NCBI Protein Information
granzyme B

NCBI Summary for GZMB

Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response. [provided by RefSeq, Jul 2008]
Show more

UniProt Comments for GZMB

GZMB: This enzyme is necessary for target cell lysis in cell- mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. By staphylococcal enterotoxin A (SEA) in peripheral blood leukocytes. Inactivated by the serine protease inhibitor diisopropylfluorophosphate. Belongs to the peptidase S1 family. Granzyme subfamily.

Protein type: EC 3.4.21.79; Apoptosis; Protease

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: membrane; intracellular membrane-bound organelle; cytoplasm; immunological synapse; cytosol; nucleus

Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: Notch signaling pathway; induction of apoptosis by granzyme; apoptosis; natural killer cell mediated cytotoxicity; cytolysis; protein processing; immune response; proteolysis
Show more

Research Articles on GZMB

1. CD4+ T cell-derived IL-21 and deprivation of CD40 signaling favor the in vivo development of granzyme B-expressing regulatory B cells in HIV patients.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GZMB antibody

 Reference Product  PubMed Publications
 CASP3 antibody  >147 publications with GZMB and CASP3
 FOXP3 antibody  >82 publications with GZMB and FOXP3
 BID antibody  >62 publications with GZMB and BID
 SERPINB9 antibody  >60 publications with GZMB and SERPINB9
 PRF1 antibody  >38 publications with GZMB and PRF1
 CASP7 antibody  >20 publications with GZMB and CASP7
 IL2RB antibody  >14 publications with GZMB and IL2RB
 IL2 antibody  >6 publications with GZMB and IL2
 CD8A antibody  >1 publications with GZMB and CD8A

Diseases associated with anti-GZMB antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >324 publications with GZMB and Necrosis
 Inflammation Antibodies  >220 publications with GZMB and Inflammation
 Skin Diseases Antibodies  >165 publications with GZMB and Skin Diseases
 Disease Models, Animal Antibodies  >131 publications with GZMB and Disease Models, Animal
 Neoplasms, Experimental Antibodies  >105 publications with GZMB and Neoplasms, Experimental
 Liver Diseases Antibodies  >84 publications with GZMB and Liver Diseases
 Hepatitis Antibodies  >56 publications with GZMB and Hepatitis
 Hypersensitivity Antibodies  >51 publications with GZMB and Hypersensitivity
 Brain Diseases Antibodies  >39 publications with GZMB and Brain Diseases
 Breast Neoplasms Antibodies  >35 publications with GZMB and Breast Neoplasms

Organs/Tissues associated with anti-GZMB antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >2205 publications with GZMB and Blood
 Lung Antibodies  >167 publications with GZMB and Lung
 Kidney Antibodies  >152 publications with GZMB and Kidney
 Spleen Antibodies  >145 publications with GZMB and Spleen
 Liver Antibodies  >145 publications with GZMB and Liver
 Bone Marrow Antibodies  >100 publications with GZMB and Bone Marrow
 Heart Antibodies  >95 publications with GZMB and Heart
 Intestine Antibodies  >69 publications with GZMB and Intestine
 Pancreas Antibodies  >34 publications with GZMB and Pancreas
 Uterus Antibodies  >22 publications with GZMB and Uterus
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000