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HEYL Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6306278

This gene encodes a member of the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcription factors. The sequence of the encoded protein contains a conserved bHLH and orange domain, but its YRPW motif has diverged… More details

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Product Overview

Online Datasheet in PDF
Product Name
HEYL, Polyclonal Antibody
Full Product Name
HEYL, NT (HEYL, BHLHB33, HRT3, Hairy/enhancer-of-split related with YRPW motif-like protein, Class B basic helix-loop-helix protein 33, Hairy-related transcription factor 3) (Azide free) (HRP)
Product Gene Name
anti-HEYL antibody
Product Synonym Names
HEYL; BHLHB33; HRT3; Hairy/enhancer-of-split related with YRPW motif-like protein; Class B basic helix-loop-helix protein 33; Hairy-related transcription factor 3
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
35,087 Da
OMIM
609034
3D Structure
ModBase 3D Structure for Q9NQ87
Immunogen
HEYL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 25-54 amino acids from the N-terminal region of human HEYL.
Conjugate
HRP
Note
Preservative Free

Product Categories/Family for anti-HEYL antibody

Antibodies; Transcription Factors
Applications Tested/Suitable for anti-HEYL antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-HEYL antibody
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-HEYL antibody

HEYL Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HEYL antibody detected the HEYL protein (arrow).
Rabbit Anti-HEYL Polyclonal Antibody – Cat# MBS6306278

Immunofluorescence (IF) of anti-HEYL antibody

Fluorescent confocal image of Hela cell stained with HEYL Antibody (N-term).HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HEYL primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). HEYL immunoreactivity is localized to Mitochondria significantly.
Rabbit Anti-HEYL Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS6306278

Product References and Citations for anti-HEYL antibody

Kokubo, H., et al. Trends Cardiovasc. Med. 15(5):190-194(2005). Colland, F., et al. Genome Res. 14(7):1324-1332(2004). Iso, T., et al. J. Cell. Physiol. 194(3):237-255(2003). Nakagawa, O., et al. Proc. Natl. Acad. Sci. U.S.A. 97(25):13655-13660(2000). Firulli, B.A., et al. J. Biol. Chem. 275(43):33567-33573(2000).

Other Notes

Small volumes of anti-HEYL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HEYL purchase

MBS6306278 is an antibody targeting HEYL. Also known as HEYL, NT (HEYL, BHLHB33, HRT3, Hairy/enhancer-of-split related with YRPW motif-like protein, Class B basic helix-loop-helix protein 33, Hairy-related transcription factor 3) (Azide free) (HRP). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for HEYL. It may not necessarily be applicable to this product.

NCBI GI #
105990530
NCBI GeneID
26508
NCBI Accession #
NP_055386.1
UniProt Primary Accession #
Q9NQ87
UniProt Secondary Accession #
Q5TG99
UniProt Related Accession #
Q9NQ87
GenBank Nucleotide #
NM_014571
NCBI Official Full Name
Homo sapiens hes-related family bHLH transcription factor with YRPW motif-like (HEYL), mRNA
NCBI Official Synonym Full Names
hes-related family bHLH transcription factor with YRPW motif-like
NCBI Official Symbol
HEYL
NCBI Official Synonym Symbols
HEY3; HRT3; HESR3; bHLHb33
UniProt Protein Name
Hairy/enhancer-of-split related with YRPW motif-like protein
UniProt Synonym Protein Names
Class B basic helix-loop-helix protein 33; bHLHb33; Hairy-related transcription factor 3; HRT-3; hHRT3
Protein Family
Hairy/enhancer-of-split related with YRPW motif-like protein
UniProt Gene Name
HEYL
UniProt Synonym Gene Names
BHLHB33; HRT3; hHeyL; bHLHb33; HRT-3; hHRT3
UniProt Entry Name
HEYL_HUMAN
NCBI Protein Information
hairy/enhancer-of-split related with YRPW motif-like protein; HEY-like protein; HRT-3; class B basic helix-loop-helix protein 33; hHRT3; hHeyL; hairy-related transcription factor 3; hairy/enhancer-of-split related with YRPW motif 3

NCBI Summary for HEYL

This gene encodes a member of the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcription factors. The sequence of the encoded protein contains a conserved bHLH and orange domain, but its YRPW motif has diverged from other HESR family members. It is thought to be an effector of Notch signaling and a regulator of cell fate decisions. Alternatively spliced transcript variants have been found, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
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Research Articles on HEYL

1. Repression of androgen receptor activity by HEYL, a third member of the Hairy/Enhancer-of-split-related family of Notch effectors.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-HEYL antibody

 Reference Product  PubMed Publications
 HEY1 antibody  >36 publications with HEYL and HEY1
 HEY2 antibody  >28 publications with HEYL and HEY2
 NOTCH1 antibody  >10 publications with HEYL and NOTCH1

Pathways associated with anti-HEYL antibody

 Products by Pathway  Pathway Diagram
 Constitutive Signaling By NOTCH1 HD+PEST Domain Mutants Pathway antibodies  Constitutive Signaling By NOTCH1 HD+PEST Domain Mutants Pathway Diagram
 Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway antibodies  Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 FBXW7 Mutants And NOTCH1 In Cancer Pathway antibodies  FBXW7 Mutants And NOTCH1 In Cancer Pathway Diagram
 NOTCH1 Intracellular Domain Regulates Transcription Pathway antibodies  NOTCH1 Intracellular Domain Regulates Transcription Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By NOTCH Pathway antibodies  Signaling By NOTCH Pathway Diagram
 Signaling By NOTCH1 Pathway antibodies  Signaling By NOTCH1 Pathway Diagram
 Signaling By NOTCH1 HD Domain Mutants In Cancer Pathway antibodies  Signaling By NOTCH1 HD Domain Mutants In Cancer Pathway Diagram
 Signaling By NOTCH1 HD+PEST Domain Mutants In Cancer Pathway antibodies  Signaling By NOTCH1 HD+PEST Domain Mutants In Cancer Pathway Diagram

Diseases associated with anti-HEYL antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >23 publications with HEYL and Nervous System Diseases
 Poisoning Antibodies  >13 publications with HEYL and Poisoning
 Lung Diseases Antibodies  >13 publications with HEYL and Lung Diseases
 Congenital Abnormalities Antibodies  >10 publications with HEYL and Congenital Abnormalities
 Necrosis Antibodies  >9 publications with HEYL and Necrosis
 Kidney Diseases Antibodies  >8 publications with HEYL and Kidney Diseases
 Fetal Death Antibodies  >8 publications with HEYL and Fetal Death
 Breast Neoplasms Antibodies  >8 publications with HEYL and Breast Neoplasms
 Fetal Growth Retardation Antibodies  >6 publications with HEYL and Fetal Growth Retardation
 Brain Diseases Antibodies  >5 publications with HEYL and Brain Diseases

Organs/Tissues associated with anti-HEYL antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >23 publications with HEYL and Brain
 Heart Antibodies  >21 publications with HEYL and Heart
 Lung Antibodies  >18 publications with HEYL and Lung
 Muscle Antibodies  >14 publications with HEYL and Muscle
 Kidney Antibodies  >12 publications with HEYL and Kidney
 Liver Antibodies  >12 publications with HEYL and Liver
 Bone Antibodies  >10 publications with HEYL and Bone
 Embryonic Tissue Antibodies  >10 publications with HEYL and Embryonic Tissue
 Placenta Antibodies  >9 publications with HEYL and Placenta
 Uterus Antibodies  >6 publications with HEYL and Uterus
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