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HIPK3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6306571

HIPK3 negatively regulates apoptosis by promoting FADD phosphorylation. This kinase enhances androgen receptor-mediated transcription, and may act as a transcriptional corepressor for NK homeodomain transcription factors. More details

$1060 / 0.2 mL +1 FREE 8GB USB
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
HIPK3, Polyclonal Antibody
Full Product Name
HIPK3, CT (HIPK3, DYRK6, FIST3, PKY, Homeodomain-interacting protein kinase 3, Androgen receptor-interacting nuclear protein kinase, Fas-interacting serine/threonine-protein kinase, Homolog of protein kinase YAK1) (AP)
Product Gene Name
Product Synonym Names
HIPK3; DYRK6; FIST3; PKY; Homeodomain-interacting protein kinase 3; Androgen receptor-interacting nuclear protein kinase; Fas-interacting serine/threonine-protein kinase; Homolog of protein kinase YAK1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Purity/Purification
Purified by Protein G Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
131,256 Da
OMIM
604424
3D Structure
ModBase 3D Structure for Q9H422
Immunogen
HIPK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1134~1163 amino acids from the sequence of human HIPK3 within a 1100-1200.
Conjugate
AP
Note
Preservative Free

Product Categories/Family for anti-HIPK3 antibody

Applications Tested/Suitable for anti-HIPK3 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes for anti-HIPK3 antibody
IHC: 1:50-100
ELISA: 1:1,000
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-HIPK3 antibody

Detection of interaction between HIPK3 and SF-1 by coimmunoprecipitation. After expression of SF-1-HA and DYKDDDDK-sHIPK3 (aa 159 to 1191) in H1299 cells, the HIPK3 protein complex was immunoprecipitated with anti-DYKDDDDK antibody or by direct loading to the gel (input). Western blotting was then performed to detect SF-1-HA and DYKDDDDK-sHIPK3.
Rabbit Anti-HIPK3 Polyclonal Antibody – Cat# MBS6306571

Immunohistochemistry (IHC) of anti-HIPK3 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Rabbit Anti-HIPK3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6306571

Product References and Citations for anti-HIPK3 antibody

Blume-Jensen P, et al. Nature 2001. 411: 355. Cantrell D, J. Cell Sci. 2001. 114: 1439. Jhiang S Oncogene 2000. 19: 5590. Manning G, et al. Science 2002. 298: 1912. Moller, D, et al. Am. J. Physiol. 1994. 266: C351-C359. Robertson, S. et al. Trends Genet. 2000. 16: 368. Robinson D, et al. Oncogene 2000. 19: 5548. Van der Ven, P, et al. Hum. Molec. Genet. 1993. 2: 1889. Vanhaesebroeck, B, et al. Biochem. J. 2000. 346: 561. Van Weering D, et al. Recent Results Cancer Res. 1998. 154: 271.

Other Notes

Small volumes of anti-HIPK3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HIPK3 purchase

MBS6306571 is an antibody targeting HIPK3. Also known as HIPK3, CT (HIPK3, DYRK6, FIST3, PKY, Homeodomain-interacting protein kinase 3, Androgen receptor-interacting nuclear protein kinase, Fas-interacting serine/threonine-protein kinase, Homolog of protein kinase YAK1) (AP). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for HIPK3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
homeodomain-interacting protein kinase 3 isoform 1
NCBI Official Synonym Full Names
homeodomain interacting protein kinase 3
NCBI Official Symbol
HIPK3
NCBI Official Synonym Symbols
PKY; YAK1; DYRK6; FIST3
UniProt Protein Name
Homeodomain-interacting protein kinase 3
UniProt Synonym Protein Names
Androgen receptor-interacting nuclear protein kinase; ANPK; Fas-interacting serine/threonine-protein kinase; FIST; Homolog of protein kinase YAK1
UniProt Gene Name
HIPK3
UniProt Synonym Gene Names
DYRK6; FIST3; PKY; ANPK; FIST
UniProt Entry Name
HIPK3_HUMAN
NCBI Protein Information
homeodomain-interacting protein kinase 3; ANPK; FIST; homolog of protein kinase YAK1; fas-interacting serine/threonine-protein kinase; androgen receptor-interacting nuclear protein kinase

UniProt Comments for HIPK3

HIPK3: homeodomain interacting protein kinase 3 is a CMGC kinase of the DYRK family. Phosphorylates FADD and inhibits fas-mediated Jun NH(2)-terminal kinase activation.

Protein type: Kinase, protein; Nuclear receptor co-regulator; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; EC 2.7.11.1; CMGC group; DYRK family; HIPK subfamily

Chromosomal Location of Human Ortholog: 11p13

Cellular Component: PML body; cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; ATP binding; protein kinase activity

Biological Process: negative regulation of JNK activity; peptidyl-serine phosphorylation; regulation of transcription, DNA-dependent; apoptosis; peptidyl-threonine phosphorylation; mRNA transcription; protein amino acid phosphorylation; negative regulation of apoptosis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-HIPK3 antibody

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