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HIST1H1D Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS7110705

MBS7110705 is an antibody targeting HIST1H1D. Also known as HIST1H1D(Ab-106) Antibody. Reactive in Human. Host: Rabbit. More details

$235 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HIST1H1D, Polyclonal Antibody
Full Product Name
HIST1H1D(Ab-106) Antibody
Product Gene Name
anti-HIST1H1D antibody
Product Synonym Names
Histone H1.3; Histone H1c; Histone H1s-2; HIST1H1D; H1F3
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid
Purity/Purification
Antigen Affinity Purified
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
Molecular Weight
22,350 Da
OMIM
M60747 Genomic DNA
3D Structure
ModBase 3D Structure for P16402
Immunogen
Peptide sequence around site of beta?hydroxybutyryl-Lys(106) derived from human Histone H1.3
Conjugation
Non-conjugated
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Product Categories/Family for anti-HIST1H1D antibody

Epigenetics and Nuclear Signaling
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-HIST1H1D antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-HIST1H1D antibody
WB: 1:500-1:5000
IHC: 1:10-1:100

Western Blot (WB) of anti-HIST1H1D antibody

Western Blot
Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate
All lanes: HIST1H1D antibody at 1.25ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 23 KDa
Observed band size: 23 KDa
Rabbit Anti-HIST1H1D Polyclonal Antibody – Cat# MBS7110705

Immunohistochemistry (IHC) of anti-HIST1H1D antibody

IHC image of MBS7110705 diluted at 1:20 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Rabbit Anti-HIST1H1D Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS7110705

Other Notes

Small volumes of anti-HIST1H1D antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HIST1H1D. It may not necessarily be applicable to this product.

NCBI GI #
4885377
NCBI GeneID
3007
NCBI Accession #
NP_005311.1
UniProt Primary Accession #
P16402
UniProt Secondary Accession #
Q2M2I2; B2R751
UniProt Related Accession #
P16402
GenBank Nucleotide #
NM_005320.2
NCBI Official Full Name
histone H1.3
NCBI Official Synonym Full Names
histone cluster 1 H1 family member d
NCBI Official Symbol
HIST1H1D
NCBI Official Synonym Symbols
H1D; H1.3; H1F3; H1s-2
UniProt Protein Name
Histone H1.3
UniProt Synonym Protein Names
Histone H1c; Histone H1s-2
UniProt Gene Name
HIST1H1D
UniProt Synonym Gene Names
H1F3
NCBI Protein Information
histone H1.3

NCBI Summary for HIST1H1D

Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015]
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UniProt Comments for HIST1H1D

Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation ().
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Research Articles on HIST1H1D

1. Results show that histone H1.3, was identified only in non-neoplastic MCF-10A breast cells but not in metastatic MDA-MB-231 breast cancer cells .

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-HIST1H1D antibody

 Products by Pathway  Pathway Diagram
 Activation Of DNA Fragmentation Factor Pathway antibodies  Activation Of DNA Fragmentation Factor Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Induced DNA Fragmentation Pathway antibodies  Apoptosis Induced DNA Fragmentation Pathway Diagram
 Apoptotic Execution Phase Pathway antibodies  Apoptotic Execution Phase Pathway Diagram

Diseases associated with anti-HIST1H1D antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >1 publications with HIST1H1D and Neoplasms
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