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Histone H3 (H3) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249666

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string'… More details

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Product Overview

Product Name
Histone H3, Polyclonal Antibody
Full Product Name
Anti-Histone H3 Antibody IHC-plus
Product Gene Name
Product Synonym Names
Rabbit Polyclonal to Human Histone H3; Human Histone H3
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
PBS, 0.09% sodium azide
Purity/Purification
Immunoaffinity purified
Specificity
Human Histone H3
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months.
For long term storage store at -20 degree C.
Molecular Weight
15,406 Da
Immunogen
Histone H3 antibody was raised against kLH-conjugated synthetic peptide selected from the K9 region of human histone H3.
Immunogen Description
KLH-conjugated synthetic peptide selected from the K9 region of human histone H3.
Immunogen Type
Synthetic peptide
Target Species
Human
Applications Tested/Suitable for anti-H3 antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-H3 antibody
Flow Cytometry (1:10 - 1:50)
IHC-P (1:100)
WB (1:1000)

Immunohistochemistry (IHC) of anti-H3 antibody

Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
Rabbit Anti-H3 Polyclonal Antibody – Cat# MBS249666

Immunohistochemistry (IHC) of anti-H3 antibody

Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Rabbit Anti-H3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS249666

Western Blot (WB) of anti-H3 antibody

Western blot of Methyl-K-H3-K9(2Me)-4MAPS in K562 cell line lysates (35 ug/lane). H3 (arrow) was detected using the purified antibody.
Rabbit Anti-H3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS249666

Flow Cytometry (FC/FACS) of anti-H3 antibody

Histone H3-K9 Antibody flow cytometry of K562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-H3 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS249666

Other Notes

Small volumes of anti-H3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for H3 purchase

MBS249666 is an antibody targeting H3. Also known as Anti-Histone H3 Antibody IHC-plus. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for H3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
NCBI Official Full Name
histone H3
UniProt Protein Name
Histone H3.3
Protein Family
UniProt Gene Name
H3
UniProt Entry Name
H33_TOBAC

UniProt Comments for H3

Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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