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HRK Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6481166

MBS6481166 is an antibody targeting Hrk. Also known as Hrk, BH3 Domain (Activator of Apoptosis Harakiri, Neuronal Death Protein DP5, BH3-interacting Domain-containing Protein 3, BID3) (Azide free) (HRP). Reactive in Human. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Hrk, Polyclonal Antibody
Full Product Name
Hrk, BH3 Domain (Activator of Apoptosis Harakiri, Neuronal Death Protein DP5, BH3-interacting Domain-containing Protein 3, BID3) (Azide free) (HRP)
Product Gene Name
anti-Hrk antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Purity/Purification
Purified by Protein G Affinity Chromatography.
Specificity
Recognizes human Hrk.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
9,884 Da
OMIM
603447
3D Structure
ModBase 3D Structure for O00198
Immunogen
Synthetic peptide selected from a portion within aa30-50 of human Hrk (KLH).
Conjugate
HRP
Note
Preservative Free

Product Categories/Family for anti-Hrk antibody

Antibodies; Apoptosis
Applications Tested/Suitable for anti-Hrk antibody
Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-Hrk antibody
IHC: 1:50-1:100
ELISA: 1:1,000
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-Hrk antibody

Hrk-BH3 western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the Hrk antibody detected the Hrk protein (arrow).
Rabbit Anti-HRK Polyclonal Antibody – Cat# MBS6481166

Immunohistochemistry (IHC) of anti-Hrk antibody

Hrk BH3 Domain Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Hrk BH3 Domain Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-HRK Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6481166

Product References and Citations for anti-Hrk antibody

1. Wakabayashi, T., et al., Neurosci. Lett. 318(2):77-80 (2002). 2. Inohara, N., et al., EMBO J. 16(7):1686-1694 (1997).

Other Notes

Small volumes of anti-Hrk antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Hrk. It may not necessarily be applicable to this product.

NCBI GI #
4504493
NCBI GeneID
8739
NCBI Accession #
NP_003797
UniProt Primary Accession #
O00198
UniProt Related Accession #
O00198
GenBank Nucleotide #
NM_003806.2
NCBI Official Full Name
activator of apoptosis harakiri
NCBI Official Synonym Full Names
harakiri, BCL2 interacting protein
NCBI Official Symbol
HRK
NCBI Official Synonym Symbols
DP5; HARAKIRI
UniProt Protein Name
Activator of apoptosis harakiri
UniProt Synonym Protein Names
BH3-interacting domain-containing protein 3; Neuronal death protein DP5
Protein Family
Activator of apoptosis harakiri
UniProt Gene Name
HRK
UniProt Synonym Gene Names
BID3
UniProt Entry Name
HRK_HUMAN
NCBI Protein Information
activator of apoptosis harakiri; death protein 5; BCL2-interacting protein; activator of apoptosis Hrk; neuronal death protein DP5; BH3-interacting domain-containing protein 3; harakiri, BCL2 interacting protein (contains only BH3 domain)

NCBI Summary for Hrk

This gene encodes a member of the BCL-2 protein family. Members of this family are involved in activating or inhibiting apoptosis. The encoded protein localizes to intracellular membranes. This protein promotes apoptosis by interacting with the apoptotic inhibitors BCL-2 and BCL-X(L) via its BH3 domain. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012]
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UniProt Comments for Hrk

HRK: Promotes apoptosis.

Protein type: Membrane protein, integral; Apoptosis

Chromosomal Location of Human Ortholog: 12q24.22

Cellular Component: mitochondrion; integral to membrane

Molecular Function: protein binding

Biological Process: apoptosis; positive regulation of apoptosis; positive regulation of protein homooligomerization
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Research Articles on Hrk

1. our findings suggest that induction of the BH3-only protein Hrk is a critical step in 2-ME activation of the JNK-induced apoptotic pathway, targeting mitochondria by liberating proapoptotic protein Bak.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Hrk antibody

 Reference Product  PubMed Publications
 JUN antibody  >32 publications with Hrk and JUN
 BAD antibody  >16 publications with Hrk and BAD
 BCL2L1 antibody  >14 publications with Hrk and BCL2L1
 BIK antibody  >10 publications with Hrk and BIK
 BMF antibody  >8 publications with Hrk and BMF

Pathways associated with anti-Hrk antibody

 Products by Pathway  Pathway Diagram
 ATF-2 Transcription Factor Network Pathway antibodies  ATF-2 Transcription Factor Network Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Modulation And Signaling Pathway antibodies  Apoptosis Modulation And Signaling Pathway Diagram

Diseases associated with anti-Hrk antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >20 publications with Hrk and Nervous System Diseases
 Brain Diseases Antibodies  >8 publications with Hrk and Brain Diseases
 Inflammation Antibodies  >6 publications with Hrk and Inflammation
 Cardiovascular Diseases Antibodies  >5 publications with Hrk and Cardiovascular Diseases
 Breast Neoplasms Antibodies  >5 publications with Hrk and Breast Neoplasms
 Necrosis Antibodies  >5 publications with Hrk and Necrosis
 Disease Models, Animal Antibodies  >3 publications with Hrk and Disease Models, Animal
 Mitochondrial Diseases Antibodies  >3 publications with Hrk and Mitochondrial Diseases
 Seizures Antibodies  >2 publications with Hrk and Seizures
 Hepatitis Antibodies  >2 publications with Hrk and Hepatitis

Organs/Tissues associated with anti-Hrk antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >21 publications with Hrk and Brain
 Lymph Node Antibodies  >2 publications with Hrk and Lymph Node
 Testis Antibodies  >1 publications with Hrk and Testis
 Thyroid Antibodies  >1 publications with Hrk and Thyroid
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