Rabbit Anti-HSD11B1 Polyclonal Antibody – Cat# MBS1754939

Figure 1. Western blot analysis of HSD11B1 using anti-HSD11B1 antibody (MBS1754939).

Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.

Lane 1: human HCCT tissue lysates,
Lane 2: human HCCP tissue lysates.

After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD11B1 antigen affinity purified polyclonal antibody (#MBS1754939) at 0.25 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HSD11B1 at approximately 36 kDa. The expected band size for HSD11B1 is at 32 kDa.

HSD11B1 Antibody – Rabbit Polyclonal

Name: HSD11B1 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1754939
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1754939

Background: CCL1, Chemokine(C-C motif) ligand 1, is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is… More details

$210 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

HSD11B1, Polyclonal Antibody

Full Product Name

Anti-HSD11B1 Antibody Picoband

Product Gene Name

anti-HSD11B1 antibody

Clonality

Polyclonal

Isotype

Rabbit IgG

Host

Rabbit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

15.6 pg/ml - 1,000 pg/ml

Sensitivity

<4 pg/ml

Form/Format

Lyophilized
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purity/Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. (lot specific)

Specificity

Natural and recombinant Human CCL1

Preparation and Storage

Store at -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months. Avoid repeated freezing and thawing.

Inter-assay Precision

Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Intra-assay Precision

Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.

Molecular Weight

32,401 Da

Chromosome Location

Chromosome: 1; NC_000001.10 (209859525..209908295). Location: 1q32-q41

OMIM

600713

3D Structure

ModBase 3D Structure for P28845

Product Categories/Family for anti-HSD11B1 antibody

Primary Antibodies, Rabbit Polyclonal Antibodies

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-HSD11B1 antibody

Western Blot (WB), ELISA (EIA)

Application Notes for anti-HSD11B1 antibody

WB: 0.1-0.25 ug/ml, Human
Direct ELISA: 0.1-0.5 ug/ml, Human
Tested Species: In-house tested species with positive results.
Enhanced Chemiluminescent Kit with anti-Rabbit IgG (#MBS176460) for Western blot.

Other Notes

Small volumes of anti-HSD11B1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HSD11B1 purchase

MBS1754939 is an antibody targeting HSD11B1. Also known as Anti-HSD11B1 Antibody Picoband. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for HSD11B1. It may not necessarily be applicable to this product.

NCBI GI #

332078483

NCBI GeneID

3290

NCBI Accession #

NP_001193670.1

UniProt Primary Accession #

P28845

UniProt Secondary Accession #

B2R9Z1; D3DT89

UniProt Related Accession #

P28845

GenBank Nucleotide #

NM_001206741.1

NCBI Official Full Name

corticosteroid 11-beta-dehydrogenase isozyme 1

NCBI Official Synonym Full Names

hydroxysteroid (11-beta) dehydrogenase 1

NCBI Official Symbol

HSD11B1

NCBI Official Synonym Symbols

HDL; 11-DH; HSD11; HSD11B; HSD11L; CORTRD2; SDR26C1; 11-beta-HSD1

UniProt Protein Name

Corticosteroid 11-beta-dehydrogenase isozyme 1

UniProt Synonym Protein Names

11-beta-hydroxysteroid dehydrogenase 1

Protein Family

Corticosteroid 11-beta-dehydrogenase isozyme

UniProt Gene Name

HSD11B1

UniProt Synonym Gene Names

HSD11; HSD11L; 11-DH

UniProt Entry Name

DHI1_HUMAN

NCBI Protein Information

corticosteroid 11-beta-dehydrogenase isozyme 1; short chain dehydrogenase/reductase family 26C, member 1

NCBI Summary for HSD11B1

The protein encoded by this gene is a microsomal enzyme that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. In addition, the encoded protein can catalyze the reverse reaction, the conversion of cortisone to cortisol. Too much cortisol can lead to central obesity, and a particular variation in this gene has been associated with obesity and insulin resistance in children. Mutations in this gene and H6PD (hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase)) are the cause of cortisone reductase deficiency. Alternate splicing results in multiple transcript variants encoding the same protein.[provided by RefSeq, May 2011]

UniProt Comments for HSD11B1

HSD11B1: Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7- ketocholesterol to 7-beta-hydroxycholesterol. Defects in HSD11B1 are a cause of cortisone reductase deficiency (CRD). In CRD, activation of cortisone to cortisol does not occur, resulting in adrenocorticotropin-mediated androgen excess and a phenotype resembling polycystic ovary syndrome (PCOS). Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Protein type: Lipid Metabolism - androgen and estrogen; Membrane protein, integral; Endoplasmic reticulum; Oxidoreductase; Lipid Metabolism - C21-steroid hormone; EC 1.1.1.146

Chromosomal Location of Human Ortholog: 1q32-q41

Cellular Component: endoplasmic reticulum membrane; membrane; integral to membrane

Molecular Function: 11-beta-hydroxysteroid dehydrogenase activity

Biological Process: steroid metabolic process; glucocorticoid biosynthetic process; lung development

Disease: Cortisone Reductase Deficiency 2

Research Articles on HSD11B1

1. in adipose tissue, estrogen receptor beta-mediated estrogen signaling can up-regulate 11 beta-Hydroxysteroid dehydrogenase type I and that this may be of particular importance in postmenopausal women

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-HSD11B1 antibody

Products by Pathway	Pathway Diagram
C21-Steroid Hormone Biosynthesis, Progesterone => Cortisol/cortisone Pathway antibodies	C21-Steroid Hormone Biosynthesis, Progesterone => Cortisol/cortisone Pathway Diagram
C21-Steroid Hormone Biosynthesis, Progesterone => Cortisol/cortisone Pathway antibodies	C21-Steroid Hormone Biosynthesis, Progesterone => Cortisol/cortisone Pathway Diagram
Chemical Carcinogenesis Pathway antibodies	Chemical Carcinogenesis Pathway Diagram
Chemical Carcinogenesis Pathway antibodies	Chemical Carcinogenesis Pathway Diagram
Glucocorticoid & Mineralcorticoid Metabolism Pathway antibodies	Glucocorticoid & Mineralcorticoid Metabolism Pathway Diagram
Glucocorticoid Biosynthesis Pathway antibodies	Glucocorticoid Biosynthesis Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Lipids And Lipoproteins Pathway antibodies	Metabolism Of Lipids And Lipoproteins Pathway Diagram
Metabolism Of Steroid Hormones And Vitamin D Pathway antibodies	Metabolism Of Steroid Hormones And Vitamin D Pathway Diagram
Metabolism Of Xenobiotics By Cytochrome P450 Pathway antibodies	Metabolism Of Xenobiotics By Cytochrome P450 Pathway Diagram

Diseases associated with anti-HSD11B1 antibody

Disease Name	Pubmed Publications
Obesity Antibodies	>73 publications with HSD11B1 and Obesity
Hypertension Antibodies	>25 publications with HSD11B1 and Hypertension
Cardiovascular Diseases Antibodies	>20 publications with HSD11B1 and Cardiovascular Diseases
Liver Diseases Antibodies	>14 publications with HSD11B1 and Liver Diseases
Obesity, Abdominal Antibodies	>13 publications with HSD11B1 and Obesity, Abdominal
Inflammation Antibodies	>12 publications with HSD11B1 and Inflammation
Cortisone reductase deficiency Antibodies	>11 publications with HSD11B1 and Cortisone reductase deficiency
Necrosis Antibodies	>9 publications with HSD11B1 and Necrosis
Kidney Diseases Antibodies	>8 publications with HSD11B1 and Kidney Diseases
Brain Diseases Antibodies	>7 publications with HSD11B1 and Brain Diseases

Organs/Tissues associated with anti-HSD11B1 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>55 publications with HSD11B1 and Blood
Liver Antibodies	>52 publications with HSD11B1 and Liver
Connective Tissue Antibodies	>43 publications with HSD11B1 and Connective Tissue
Ovary Antibodies	>20 publications with HSD11B1 and Ovary
Kidney Antibodies	>17 publications with HSD11B1 and Kidney
Brain Antibodies	>15 publications with HSD11B1 and Brain
Muscle Antibodies	>13 publications with HSD11B1 and Muscle
Lung Antibodies	>11 publications with HSD11B1 and Lung
Uterus Antibodies	>7 publications with HSD11B1 and Uterus
Placenta Antibodies	>6 publications with HSD11B1 and Placenta