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HSD11B2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6308445

There are at least two isozymes of the corticosteroid 11-beta-dehydrogenase, a microsomal enzyme complex responsible for the interconversion of cortisol and cortisone. The type I isozyme has both 11-beta-dehydrogenase (cortisol to cortisone) and… More details

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Product Overview

Online Datasheet in PDF
Product Name
HSD11B2, Polyclonal Antibody
Full Product Name
HSD11B2, ID (HSD11B2, HSD11K, Corticosteroid 11-beta-dehydrogenase isozyme 2, 11-beta-hydroxysteroid dehydrogenase type 2, 11-beta-hydroxysteroid dehydrogenase type II, NAD-dependent 11-beta-hydroxysteroid dehydrogenase) (Azide free) (HRP)
Product Gene Name
anti-HSD11B2 antibody
Product Synonym Names
HSD11B2; HSD11K; Corticosteroid 11-beta-dehydrogenase isozyme 2; 11-beta-hydroxysteroid dehydrogenase type 2; 11-beta-hydroxysteroid dehydrogenase type II; NAD-dependent 11-beta-hydroxysteroid dehydrogenase
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
44,127 Da
OMIM
218030
3D Structure
ModBase 3D Structure for P80365
Immunogen
HSD11B2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 284~314 amino acids from the Central region of human HSD11B2.
Conjugate
HRP
Note
Preservative Free

Product Categories/Family for anti-HSD11B2 antibody

Antibodies; Enzymes; Dehydrogenase
Applications Tested/Suitable for anti-HSD11B2 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes for anti-HSD11B2 antibody
IHC: 1:50-100
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-HSD11B2 antibody

Western blot analysis of HSD11B2 Antibody (Center) in MCF-7 cell line lysates (35ug/lane). HSD11B2 (arrow) was detected using the purified Pab.
Rabbit Anti-HSD11B2 Polyclonal Antibody – Cat# MBS6308445

Immunohistochemistry (IHC) of anti-HSD11B2 antibody

HSD11B2 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HSD11B2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-HSD11B2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6308445

Flow Cytometry (FC/FACS) of anti-HSD11B2 antibody

HSD11B2 Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-HSD11B2 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6308445

Product References and Citations for anti-HSD11B2 antibody

Li, J., et al. Breast Cancer Res. 12 (2), R19 (2010). Ni, X.T., et al. Placenta 30(12):1023-1028(2009). Mericq, V., et al. Eur. J. Endocrinol. 161(3):419-425(2009). Stark, M.J., et al. Am. J. Physiol. Regul. Integr. Comp. Physiol. 297 (2), R510-R514 (2009). Lepenies, J., et al. Clin. Exp. Hypertens. 31(4):376-379(2009).

Other Notes

Small volumes of anti-HSD11B2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HSD11B2 purchase

MBS6308445 is an antibody targeting HSD11B2. Also known as HSD11B2, ID (HSD11B2, HSD11K, Corticosteroid 11-beta-dehydrogenase isozyme 2, 11-beta-hydroxysteroid dehydrogenase type 2, 11-beta-hydroxysteroid dehydrogenase type II, NAD-dependent 11-beta-hydroxysteroid dehydrogenase) (Azide free) (HRP). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for HSD11B2. It may not necessarily be applicable to this product.

NCBI GI #
119392083
NCBI GeneID
3291
NCBI Accession #
NP_000187.3
UniProt Primary Accession #
P80365
UniProt Secondary Accession #
Q13194; Q6P2G9; Q8N439; Q96QN8; Q9UC50; Q9UC51; Q9UCW5; Q9UCW6; Q9UCW7; A7LB28; C5HTY7
UniProt Related Accession #
P80365
GenBank Nucleotide #
NM_000196.3
NCBI Official Full Name
corticosteroid 11-beta-dehydrogenase isozyme 2
NCBI Official Synonym Full Names
hydroxysteroid (11-beta) dehydrogenase 2
NCBI Official Symbol
HSD11B2
NCBI Official Synonym Symbols
AME; AME1; HSD2; HSD11K; SDR9C3
UniProt Protein Name
Corticosteroid 11-beta-dehydrogenase isozyme 2
UniProt Synonym Protein Names
11-beta-hydroxysteroid dehydrogenase type 2; 11-DH2; 11-beta-HSD2; 11-beta-hydroxysteroid dehydrogenase type II; 11-HSD type II; 11-beta-HSD type II; NAD-dependent 11-beta-hydroxysteroid dehydrogenase; 11-beta-HSD; Short chain dehydrogenase/reductase family 9C member 3
Protein Family
Corticosteroid 11-beta-dehydrogenase isozyme
UniProt Gene Name
HSD11B2
UniProt Synonym Gene Names
; 11-DH2; 11-beta-HSD2; 11-HSD type II; 11-beta-HSD type II; 11-beta-HSD
UniProt Entry Name
DHI2_HUMAN
NCBI Protein Information
corticosteroid 11-beta-dehydrogenase isozyme 2

UniProt Comments for HSD11B2

HSD11B2: Catalyzes the conversion of cortisol to the inactive metabolite cortisone. Modulates intracellular glucocorticoid levels, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids. Defects in HSD11B2 are the cause of apparent mineralocorticoid excess (AME). An autosomal recessive form of low-renin hypertension. It is usually diagnosed within the first years of life and is characterized by polyuria and polydipsia, failure to thrive, hypernatremia, severe hypertension with low renin and aldosterone levels, profound hypokalemia with metabolic alkalosis, and most often nephrocalcinosis. Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Protein type: EC 1.1.1.-; Lipid Metabolism - androgen and estrogen; Oxidoreductase; Lipid Metabolism - C21-steroid hormone

Chromosomal Location of Human Ortholog: 16q22

Cellular Component: endoplasmic reticulum membrane

Molecular Function: 11-beta-hydroxysteroid dehydrogenase activity; NAD binding; steroid binding

Biological Process: aldosterone mediated regulation of blood volume; female pregnancy; glucocorticoid biosynthetic process; response to drug; response to food; response to glucocorticoid stimulus; response to hypoxia; response to insulin stimulus

Disease: Apparent Mineralocorticoid Excess
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Research Articles on HSD11B2

1. No interaction was found between HSD11B2 and exposure during pregnancy, but individuals with the A allele of rs5479 had an increased risk of schizophrenia after exposure at age 3-9 years

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-HSD11B2 antibody

 Products by Pathway  Pathway Diagram
 Aldosterone-regulated Sodium Reabsorption Pathway antibodies  Aldosterone-regulated Sodium Reabsorption Pathway Diagram
 Aldosterone-regulated Sodium Reabsorption Pathway antibodies  Aldosterone-regulated Sodium Reabsorption Pathway Diagram
 C21-Steroid Hormone Biosynthesis, Progesterone => Cortisol/cortisone Pathway antibodies  C21-Steroid Hormone Biosynthesis, Progesterone => Cortisol/cortisone Pathway Diagram
 C21-Steroid Hormone Biosynthesis, Progesterone => Cortisol/cortisone Pathway antibodies  C21-Steroid Hormone Biosynthesis, Progesterone => Cortisol/cortisone Pathway Diagram
 Glucocorticoid & Mineralcorticoid Metabolism Pathway antibodies  Glucocorticoid & Mineralcorticoid Metabolism Pathway Diagram
 Glucocorticoid Biosynthesis Pathway antibodies  Glucocorticoid Biosynthesis Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Metabolism Of Steroid Hormones Pathway antibodies  Metabolism Of Steroid Hormones Pathway Diagram
 Prostaglandin Synthesis And Regulation Pathway antibodies  Prostaglandin Synthesis And Regulation Pathway Diagram

Diseases associated with anti-HSD11B2 antibody

 Disease Name  Pubmed Publications
 Hypertension Antibodies  >111 publications with HSD11B2 and Hypertension
 Cardiovascular Diseases Antibodies  >67 publications with HSD11B2 and Cardiovascular Diseases
 Apparent mineralocorticoid excess Antibodies  >54 publications with HSD11B2 and Apparent mineralocorticoid excess
 Mineralocorticoid Excess Syndrome, Apparent Antibodies  >42 publications with HSD11B2 and Mineralocorticoid Excess Syndrome, Apparent
 Pregnancy Complications Antibodies  >29 publications with HSD11B2 and Pregnancy Complications
 Kidney Diseases Antibodies  >15 publications with HSD11B2 and Kidney Diseases
 Inflammation Antibodies  >11 publications with HSD11B2 and Inflammation
 Renal Insufficiency Antibodies  >10 publications with HSD11B2 and Renal Insufficiency
 Necrosis Antibodies  >7 publications with HSD11B2 and Necrosis
 Hyperplasia Antibodies  >7 publications with HSD11B2 and Hyperplasia

Organs/Tissues associated with anti-HSD11B2 antibody

 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >75 publications with HSD11B2 and Kidney
 Placenta Antibodies  >67 publications with HSD11B2 and Placenta
 Liver Antibodies  >27 publications with HSD11B2 and Liver
 Brain Antibodies  >20 publications with HSD11B2 and Brain
 Heart Antibodies  >18 publications with HSD11B2 and Heart
 Testis Antibodies  >13 publications with HSD11B2 and Testis
 Connective Tissue Antibodies  >12 publications with HSD11B2 and Connective Tissue
 Lung Antibodies  >7 publications with HSD11B2 and Lung
 Uterus Antibodies  >6 publications with HSD11B2 and Uterus
 Intestine Antibodies  >5 publications with HSD11B2 and Intestine
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