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HSD3B7 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1759080

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Product Overview

Online Datasheet in PDF
Product Name
HSD3B7, Polyclonal Antibody
Full Product Name
Anti-HSD3B7 Antibody Picoband
Product Gene Name
anti-HSD3B7 antibody
Product Synonym Names
Serine/threonine-protein kinase AtPK1/AtPK6; 70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70(S6K) alpha antibody, p70(S6K)-alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Specificity
Expressed in all tissues.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
Molecular Weight
41,016 Da
Chromosome Location
Chromosome: 16; NC_000016.9 (30996519..31000473). Location: 16p11.2
OMIM
607764
3D Structure
ModBase 3D Structure for Q9H2F3
Immunogen
E coli-derived human HSD3B7 recombinant protein (Position: E23-A363). Human HSD3B7 shares 87.7% amino acid (aa) sequence identity with mouse HSD3B7.
Subcellular Localization
Cytoplasm, Nucleus
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross reactivity with other proteins.
Protein Function
Downstream effector of TOR signaling pathway involved in osmotic stress response. Could be involved in the control of plant growth and development. Phosphorylates the ribosomal proteins P14, P16 and S6. Functions as a repressor of cell proliferation and required for maintenance of chromosome stability and ploidy levels through the RBR1-E2F pathway.

Product Categories/Family for anti-HSD3B7 antibody

Primary Antibodies; Signal Transduction; Signaling Pathway; Small G Proteins
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-HSD3B7 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-HSD3B7 antibody
WB: 0.25-0.5ug/ml, Human
ICC/IF: 5ug/ml, Human
ELISA: 0.1-0.5ug/ml

Western Blot (WB) of anti-HSD3B7 antibody

Figure 1. Western blot analysis of HSD3B7 using anti-HSD3B7 antibody (MBS1759080).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD3B7 antigen affinity purified polyclonal antibody (#MBS1759080) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for HSD3B7 at approximately 45 kDa. The expected band size for HSD3B7 is at 41 kDa.
Rabbit Anti-HSD3B7 Polyclonal Antibody – Cat# MBS1759080

Immunofluorescence (IF) of anti-HSD3B7 antibody

Figure 2. IF analysis of HSD3B7 using anti-HSD3B7 antibody (MBS1759080).
HSD3B7 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (#MBS176582) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-HSD3B7 Antibody (MBS1759080) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Rabbit Anti-HSD3B7 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS1759080

Product References and Citations for anti-HSD3B7 antibody

1. Cheng, J. B., Jacquemin, E., Gerhardt, M., Nazer, H., Cresteil, D., Heubi, J. E., Setchell, K. D. R., Russell, D. W. Molecular genetics of 3-beta-hydroxy-D5-C27-steroid oxidoreductase deficiency in 16 patients with loss of bile acid synthesis and liver disease. J. Clin. Endocr. Metab. 88: 1833-1841, 2003.2. Clayton, P. T., Leonard, J. V., Lawson, A. M., Setchell, K. D. R., Andersson, S., Egestad, B., Sjovall, J. Familial giant cell hepatitis associated with synthesis of 3-beta,7-alpha-dihydroxy- and 3-beta,7-alpha,12-alpha-trihydroxy-5-cholenoic acids. J. Clin. Invest. 79: 1031-1038, 1987.3. Schwarz, M., Wright, A. C., Davis, D. L., Nazer, H., Bjorkhem, I., Russell, D. W. The bile acid synthetic gene 3-beta-hydroxy-delta-5-C27-steroid oxidoreductase is mutated in progressive intrahepatic cholestasis. J. Clin. Invest. 106: 1175-1184, 2000.

Other Notes

Small volumes of anti-HSD3B7 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HSD3B7 purchase

MBS1759080 is an antibody targeting HSD3B7. Also known as Anti-HSD3B7 Antibody Picoband. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for HSD3B7. It may not necessarily be applicable to this product.

NCBI GI #
218563684
NCBI GeneID
80270
NCBI Accession #
NP_001136249.1
UniProt Primary Accession #
Q9H2F3
UniProt Secondary Accession #
Q96M28; Q9BSN9
UniProt Related Accession #
Q9H2F3
GenBank Nucleotide #
NM_001142777.1
NCBI Official Full Name
3 beta-hydroxysteroid dehydrogenase type 7 isoform b
NCBI Official Synonym Full Names
hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7
NCBI Official Symbol
HSD3B7
NCBI Official Synonym Symbols
CBAS1; PFIC4; SDR11E3
UniProt Protein Name
3 beta-hydroxysteroid dehydrogenase type 7
UniProt Synonym Protein Names
3 beta-hydroxysteroid dehydrogenase type VII; 3-beta-HSD VII; 3-beta-hydroxy-Delta(5)-C27 steroid oxidoreductase (EC:1.1.1.-); C(27) 3-beta-HSD; Cholest-5-ene-3-beta,7-alpha-diol 3-beta-dehydrogenase (EC:1.1.1.181)
Protein Family
3 beta-hydroxysteroid dehydrogenase
UniProt Gene Name
HSD3B7
UniProt Synonym Gene Names
3-beta-HSD VII; C(27) 3-beta-HSD
UniProt Entry Name
3BHS7_HUMAN
NCBI Protein Information
3 beta-hydroxysteroid dehydrogenase type 7; 3-beta-HSD VII; C(27)-3BETA-HSD; c(27) 3-beta-HSD; 3 beta-hydroxysteroid dehydrogenase type VII; 3 beta-hydroxy-delta 5-C27-steroid oxidoreductase; 3-beta-hydroxy-Delta(5)-C27 steroid oxidoreductase; cholest-5-e

NCBI Summary for HSD3B7

This gene encodes an enzyme which is involved in the initial stages of the synthesis of bile acids from cholesterol and a member of the short-chain dehydrogenase/reductase superfamily. The encoded protein is a membrane-associated endoplasmic reticulum protein which is active against 7-alpha hydrosylated sterol substrates. Mutations in this gene are associated with a congenital bile acid synthesis defect which leads to neonatal cholestasis, a form of progressive liver disease. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]
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UniProt Comments for HSD3B7

HSD3B7: The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. HSD VII is active against four 7-alpha-hydroxylated sterols. Does not metabolize several different C(19/21) steroids as substrates. Involved in bile acid synthesis. Defects in HSD3B7 are the cause of congenital bile acid synthesis defect type 1 (CBAS1); also known as neonatal progressive intrahepatic cholestasis. CBAS1 is due to a primary defect in bile synthesis leading to progressive liver disease. Clinical features include neonatal jaundice, severe intrahepatic cholestasis and cirrhosis. Belongs to the 3-beta-HSD family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.1.1.181; Membrane protein, multi-pass; Lipid Metabolism - primary bile acid biosynthesis; Oxidoreductase; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: endoplasmic reticulum membrane; integral to membrane

Molecular Function: cholest-5-ene-3-beta,7-alpha-diol 3-beta-dehydrogenase activity; 3-beta-hydroxy-delta5-steroid dehydrogenase activity

Biological Process: bile acid biosynthetic process; bile acid metabolic process

Disease: Bile Acid Synthesis Defect, Congenital, 1
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Research Articles on HSD3B7

1. Homozygosity mapping identifies a bile acid biosynthetic defect (3beta-HSD deficiency due to a frameshift mutation in HSD3B7) in an adult with cirrhosis of unknown etiology.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-HSD3B7 antibody

 Products by Pathway  Pathway Diagram
 Bile Acid And Bile Salt Metabolism Pathway antibodies  Bile Acid And Bile Salt Metabolism Pathway Diagram
 Bile Acid Biosynthesis, Cholesterol => Cholate Pathway antibodies  Bile Acid Biosynthesis, Cholesterol => Cholate Pathway Diagram
 Bile Acid Biosynthesis, Cholesterol => Cholate Pathway antibodies  Bile Acid Biosynthesis, Cholesterol => Cholate Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Primary Bile Acid Biosynthesis Pathway antibodies  Primary Bile Acid Biosynthesis Pathway Diagram
 Primary Bile Acid Biosynthesis Pathway antibodies  Primary Bile Acid Biosynthesis Pathway Diagram
 Synthesis Of Bile Acids And Bile Salts Pathway antibodies  Synthesis Of Bile Acids And Bile Salts Pathway Diagram
 Synthesis Of Bile Acids And Bile Salts Via 24-hydroxycholesterol Pathway antibodies  Synthesis Of Bile Acids And Bile Salts Via 24-hydroxycholesterol Pathway Diagram
 Synthesis Of Bile Acids And Bile Salts Via 27-hydroxycholesterol Pathway antibodies  Synthesis Of Bile Acids And Bile Salts Via 27-hydroxycholesterol Pathway Diagram

Diseases associated with anti-HSD3B7 antibody

 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >5 publications with HSD3B7 and Liver Diseases
 Cholestasis Antibodies  >3 publications with HSD3B7 and Cholestasis

Organs/Tissues associated with anti-HSD3B7 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >6 publications with HSD3B7 and Liver
 Blood Antibodies  >3 publications with HSD3B7 and Blood
 Intestine Antibodies  >1 publications with HSD3B7 and Intestine
 Bone Marrow Antibodies  >1 publications with HSD3B7 and Bone Marrow
 Brain Antibodies  >1 publications with HSD3B7 and Brain
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