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HSD3B7 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9414734

This gene encodes an enzyme which is involved in the initial stages of the synthesis of bile acids from cholesterol and a member of the short-chain dehydrogenase/reductase superfamily. The encoded protein is a membrane-associated endoplasmic reticulum protein… More details

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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
HSD3B7, Polyclonal Antibody
Full Product Name
HSD3B7 Antibody
Product Gene Name
anti-HSD3B7 antibody
Product Synonym Names
CBAS1; PFIC4; SDR11E3
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
Purity/Purification
Antigen affinity purification.
Concentration
1.8 mg/ml (lot specific)
Specificity
The antibody detects endogenous levels of total HSD3B7 protein.
Preparation and Storage
Store at -20 degree C
Molecular Weight
21,322 Da
OMIM
607764
3D Structure
ModBase 3D Structure for Q9H2F3
Immunogen Type
Recombinant Protein
Immunogen Description
Fusion protein corresponding to residues near the N terminal of human hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7
Target Name
HSD3B7

Product Categories/Family for anti-HSD3B7 antibody

Total protein Ab
Applications Tested/Suitable for anti-HSD3B7 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-HSD3B7 antibody
Western blotting: 1:500-1:2000
Immunohistochemistry: 1:50-1:200

Testing Data of anti-HSD3B7 antibody

Gel: 8%SDS-PAGE Lysate: 40ug Skov3 cell Primary antibody: 1/400 dilution Secondary antibody dilution: 1/8000 Exposure time: 10 seconds
Rabbit Anti-HSD3B7 Polyclonal Antibody – Cat# MBS9414734

Immunohistochemistry (IHC) of anti-HSD3B7 antibody

Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using at dilution 1/40.
Rabbit Anti-HSD3B7 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9414734

Other Notes

Small volumes of anti-HSD3B7 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HSD3B7 purchase

MBS9414734 is an antibody targeting HSD3B7. Also known as HSD3B7 Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for HSD3B7. It may not necessarily be applicable to this product.

NCBI GI #
218563684
NCBI GeneID
80270
NCBI Accession #
NP_001136249.1
UniProt Primary Accession #
Q9H2F3
UniProt Secondary Accession #
Q96M28; Q9BSN9
UniProt Related Accession #
Q9H2F3
GenBank Nucleotide #
NM_001142777.1
NCBI Official Full Name
3 beta-hydroxysteroid dehydrogenase type 7 isoform b
NCBI Official Synonym Full Names
hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7
NCBI Official Symbol
HSD3B7
NCBI Official Synonym Symbols
CBAS1; PFIC4; SDR11E3
UniProt Protein Name
3 beta-hydroxysteroid dehydrogenase type 7
UniProt Synonym Protein Names
3 beta-hydroxysteroid dehydrogenase type VII; 3-beta-HSD VII; 3-beta-hydroxy-Delta(5)-C27 steroid oxidoreductase (EC:1.1.1.-); C(27) 3-beta-HSD; Cholest-5-ene-3-beta,7-alpha-diol 3-beta-dehydrogenase (EC:1.1.1.181)
Protein Family
3 beta-hydroxysteroid dehydrogenase
UniProt Gene Name
HSD3B7
UniProt Synonym Gene Names
3-beta-HSD VII; C(27) 3-beta-HSD
UniProt Entry Name
3BHS7_HUMAN
NCBI Protein Information
3 beta-hydroxysteroid dehydrogenase type 7

UniProt Comments for HSD3B7

HSD3B7: The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. HSD VII is active against four 7-alpha-hydroxylated sterols. Does not metabolize several different C(19/21) steroids as substrates. Involved in bile acid synthesis. Defects in HSD3B7 are the cause of congenital bile acid synthesis defect type 1 (CBAS1); also known as neonatal progressive intrahepatic cholestasis. CBAS1 is due to a primary defect in bile synthesis leading to progressive liver disease. Clinical features include neonatal jaundice, severe intrahepatic cholestasis and cirrhosis. Belongs to the 3-beta-HSD family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Oxidoreductase; Lipid Metabolism - primary bile acid biosynthesis; EC 1.1.1.181; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: endoplasmic reticulum membrane; integral to membrane

Molecular Function: 3-beta-hydroxy-delta5-steroid dehydrogenase activity; cholest-5-ene-3-beta,7-alpha-diol 3-beta-dehydrogenase activity; protein binding

Biological Process: bile acid biosynthetic process; bile acid metabolic process

Disease: Bile Acid Synthesis Defect, Congenital, 1
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Research Articles on HSD3B7

1. Homozygosity mapping identifies a bile acid biosynthetic defect (3beta-HSD deficiency due to a frameshift mutation in HSD3B7) in an adult with cirrhosis of unknown etiology.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-HSD3B7 antibody

 Reference Product  PubMed Publications
 CYP7A1 antibody  >2 publications with HSD3B7 and CYP7A1
 CYP27A1 antibody  >1 publications with HSD3B7 and CYP27A1
 CYP8B1 antibody  >1 publications with HSD3B7 and CYP8B1
 CYP7B1 antibody  >1 publications with HSD3B7 and CYP7B1

Pathways associated with anti-HSD3B7 antibody

 Products by Pathway  Pathway Diagram
 Bile Acid And Bile Salt Metabolism Pathway antibodies  Bile Acid And Bile Salt Metabolism Pathway Diagram
 Bile Acid Biosynthesis, Cholesterol => Cholate/chenodeoxycholate Pathway antibodies  Bile Acid Biosynthesis, Cholesterol => Cholate/chenodeoxycholate Pathway Diagram
 Bile Acid Biosynthesis, Cholesterol => Cholate/chenodeoxycholate Pathway antibodies  Bile Acid Biosynthesis, Cholesterol => Cholate/chenodeoxycholate Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Primary Bile Acid Biosynthesis Pathway antibodies  Primary Bile Acid Biosynthesis Pathway Diagram
 Primary Bile Acid Biosynthesis Pathway antibodies  Primary Bile Acid Biosynthesis Pathway Diagram
 Synthesis Of Bile Acids And Bile Salts Pathway antibodies  Synthesis Of Bile Acids And Bile Salts Pathway Diagram
 Synthesis Of Bile Acids And Bile Salts Via 24-hydroxycholesterol Pathway antibodies  Synthesis Of Bile Acids And Bile Salts Via 24-hydroxycholesterol Pathway Diagram

Diseases associated with anti-HSD3B7 antibody

 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >6 publications with HSD3B7 and Liver Diseases
 Cholestasis Antibodies  >5 publications with HSD3B7 and Cholestasis
 Liver Cirrhosis Antibodies  >2 publications with HSD3B7 and Liver Cirrhosis
 Hepatomegaly Antibodies  >1 publications with HSD3B7 and Hepatomegaly
 Hyperplasia Antibodies  >1 publications with HSD3B7 and Hyperplasia

Organs/Tissues associated with anti-HSD3B7 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >11 publications with HSD3B7 and Liver
 Blood Antibodies  >5 publications with HSD3B7 and Blood
 Intestine Antibodies  >2 publications with HSD3B7 and Intestine
 Skin Antibodies  >1 publications with HSD3B7 and Skin
 Bone Marrow Antibodies  >1 publications with HSD3B7 and Bone Marrow
 Brain Antibodies  >1 publications with HSD3B7 and Brain
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