Rabbit Anti-ICT1 Polyclonal Antibody – Cat# MBS3905942

Detection of human ICT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ICT1 antibody (MBS3905942 lot 1) used for IP at 6 ug per reaction. For blotting immunoprecipitated ICT1, MBS3905943 was used at 0.4 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

ICT1 Antibody – Rabbit Polyclonal

Name: ICT1 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS3905942
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3905942

ICT1 is an essential peptidyl-tRNA hydrolase component of the mitochondrial large ribosomal subunit. Acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion,… More details

$250 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

ICT1, Polyclonal Antibody

Full Product Name

Rabbit anti-ICT1 Antibody, Affinity Purified

Product Gene Name

anti-ICT1 antibody

Product Synonym Gene Name

MRPL58

Product Synonym Names

mitochondrial large ribosomal subunit protein ICT1; MRP-L58; peptidyl-tRNA hydrolase ICT1, mitochondrial; MRPL58; mitochondrial large ribosomal subunit protein mL62; immature colon carcinoma transcript 1 protein; ICT1; DS-1; DS1; 39S ribosomal protein L58, mitochondrial; digestion substraction 1; Peptidyl-tRNA hydrolase ICT1, mitochondrial; mitochondrial ribosomal protein L58

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human

Form/Format

Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

Purity/Purification

Antigen Affinity Purified

Concentration

1000ug/ml (lot specific)

Preparation and Storage

Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

Molecular Weight

23,630 Da

OMIM

603000

3D Structure

ModBase 3D Structure for Q14197

Immunogen

Between 156 and C-terminus

Conjugation

Unconjugated

Product Categories/Family for anti-ICT1 antibody

Primary Antibodies

Applications Tested/Suitable for anti-ICT1 antibody

Immunoprecipitation (IP)

Application Notes for anti-ICT1 antibody

IP: 6ug/mg lysate

Other Notes

Small volumes of anti-ICT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ICT1 purchase

MBS3905942 is an antibody targeting ICT1. Also known as Rabbit anti-ICT1 Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ICT1. It may not necessarily be applicable to this product.

NCBI GI #

4557657

NCBI GeneID

3396

NCBI Accession #

NP_001536

UniProt Primary Accession #

Q14197

UniProt Secondary Accession #

Q53HM7; Q53Y11; B2RAD1

UniProt Related Accession #

Q14197

GenBank Nucleotide #

NP_001536.1

NCBI Official Full Name

peptidyl-tRNA hydrolase ICT1, mitochondrial isoform 1

NCBI Official Synonym Full Names

mitochondrial ribosomal protein L58

NCBI Official Symbol

MRPL58

NCBI Official Synonym Symbols

DS1; DS-1; ICT1; MRP-L58

UniProt Protein Name

Peptidyl-tRNA hydrolase ICT1, mitochondrial

UniProt Synonym Protein Names

39S ribosomal protein L58, mitochondrial; MRP-L58; Digestion substraction 1; DS-1; Immature colon carcinoma transcript 1 protein

Protein Family

1-acylglycerol-3-phosphate O-acyltransferase

UniProt Gene Name

ICT1

UniProt Synonym Gene Names

DS1; MRP-L58; DS-1

UniProt Entry Name

ICT1_HUMAN

NCBI Protein Information

peptidyl-tRNA hydrolase ICT1, mitochondrial

NCBI Summary for ICT1

The protein encoded by this gene is a peptidyl-tRNA hydrolase and a vital component of the large mitochondrial ribosome. The encoded protein serves as a ribosome release factor for this ribosome, which translates mitochondrial genes. This protein may be responsible for degrading prematurely-terminated polypeptides and for reusing stalled ribosomes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2014]

UniProt Comments for ICT1

ICT1: Essential peptidyl-tRNA hydrolase component of the mitochondrial large ribosomal subunit. Acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion, possibly in case of abortive elongation. May be involved in the hydrolysis of peptidyl-tRNAs that have been prematurely terminated and thus in the recycling of stalled mitochondrial ribosomes. Belongs to the prokaryotic/mitochondrial release factor family. ICT1 subfamily.

Protein type: Mitochondrial; EC 3.1.1.29

Chromosomal Location of Human Ortholog: 17q25.1

Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane; mitochondrial large ribosomal subunit

Molecular Function: aminoacyl-tRNA hydrolase activity; translation release factor activity, codon nonspecific

Biological Process: mitochondrial translation; organelle organization and biogenesis

Research Articles on ICT1

1. ICT1 knockdown significantly inhibited cell proliferation and migration, led to G2/M phase cell-cycle arrest, and triggered apoptosis via the intracellular AMPKa, SAPK/JNK, and PARP signaling pathways.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ICT1 antibody

Reference Product	PubMed Publications
C12orf65 antibody	>4 publications with ICT1 and C12orf65

Pathways associated with anti-ICT1 antibody

Products by Pathway	Pathway Diagram
Mitochondrial Translation Pathway antibodies	Mitochondrial Translation Pathway Diagram
Mitochondrial Translation Elongation Pathway antibodies	Mitochondrial Translation Elongation Pathway Diagram
Mitochondrial Translation Initiation Pathway antibodies	Mitochondrial Translation Initiation Pathway Diagram
Mitochondrial Translation Termination Pathway antibodies	Mitochondrial Translation Termination Pathway Diagram
Organelle Biogenesis And Maintenance Pathway antibodies	Organelle Biogenesis And Maintenance Pathway Diagram

Diseases associated with anti-ICT1 antibody

Disease Name	Pubmed Publications
Atrophy Antibodies	>1 publications with ICT1 and Atrophy

Organs/Tissues associated with anti-ICT1 antibody

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>1 publications with ICT1 and Lung
Adipose Tissue Antibodies	>1 publications with ICT1 and Adipose Tissue