Rabbit Anti-IKBKE Polyclonal Antibody – Cat# MBS1753467

Figure 1. Western blot analysis of IKKi/IKKe/IKBKE using anti-IKKi/IKKe/IKBKE antibody (MBS1753467).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HL-60 whole cell lysates
Lane 2: human U-87MG whole cell lysates
Lane 3: human A549 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-IKKi/IKKe/IKBKE antigen affinity purified polyclonal antibody (Catalog # MBS1753467) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for IKKi/IKKe/IKBKE at approximately 80KD. The expected band size for IKKi/IKKe/IKBKE is at 80KD.

Ikki/Ikke/IKBKE (IKBKE) Antibody – Rabbit Polyclonal

Name: IKBKE Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1753467
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1753467

Inhibitor of nuclear factor kappa-B kinase subunit epsilon also known as I-kappa-B kinase epsilon or IKK-epsilon is an enzyme that in humans is encoded by the IKBKE gene. IKBKE is a noncanonical I-kappa-B kinase (IKK) that is essential for regulating… More details

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Material Safety Data Sheet View PDF

Technical Datasheet View PDF

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Product Overview

Online Datasheet in PDF

Product Name

IKKi/IKKe/IKBKE, Polyclonal Antibody

Full Product Name

Anti-IKKi/IKKe/IKBKE Antibody

Product Gene Name

anti-IKBKE antibody

Product Synonym Names

IKBKE; IKKE; IKKI; KIAA0151; Inhibitor of nuclear factor kappa-B kinase subunit epsilon; I-kappa-B kinase epsilon; IKK-E; IKK-epsilon; IkBKE; EC 2. 7. 11. 10; Inducible I kappa-B kinase; IKK-i; inhibitor of nuclear factor kappa B kinase subunit epsilon

Clonality

Polyclonal

Isotype

Rabbit IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Purity/Purification

Immunogen affinity purified.

Specificity

Rabbit IgG polyclonal antibody for IKKi/IKKe/IKBKE detection.

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

716

OMIM

605048

3D Structure

ModBase 3D Structure for Q14164

Immunogen

E Coli-derived human IKKi/IKKe/IKBKE recombinant protein (Position: Q318-V716).

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Recommended Detection Systems

Recommended Detection Systems||Lab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-IKBKE antibody

Western Blot (WB), Direct ELISA (EIA)

Application Notes for anti-IKBKE antibody

WB: 0.25-0.5ug/ml|Human|
Direct ELISA: 0.1-0.5ug/ml|Human|

Product References and Citations for anti-IKBKE antibody

1. Chiang, S. -H., Bazuine, M., Lumeng, C. N., Geletka, L. M., Mowers, J., White, N. M., Ma, J. -T., Zhou, J., Qi, N., Westcott, D., Delproposto, J. B., Blackwell, T. S., Yull, F. E., Saltiel, A. R. The protein kinase IKK-epsilon regulates energy balance in obese mice. Cell 138: 961-975, 2009.
2. Hutti, J. E., Shen, R. R., Abbott, D. W., Zhou, A. Y., Sprott, K. M., Asara, J. M., Hahn, W. C., Cantley, L. C. Phosphorylation of the tumor suppressor CYLD by the breast cancer oncogene IKK-epsilon promotes cell transformation. Molec. Cell 34: 461-472, 2009.
3. Nagase, T., Seki, N., Tanaka, A., Ishikawa, K., Nomura, N. Prediction of the coding sequences of unidentified human genes. IV. The coding sequences of 40 new genes (KIAA0121-KIAA0160) deduced by analysis of cDNA clones from human cell line KG-1. DNA Res. 2: 167-174, 1995.

Other Notes

Small volumes of anti-IKBKE antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IKBKE purchase

MBS1753467 is an antibody targeting IKBKE. Also known as Anti-IKKi/IKKe/IKBKE Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for IKBKE. It may not necessarily be applicable to this product.

NCBI GI #

14548079

NCBI GeneID

9641

NCBI Accession #

Q14164.1

UniProt Primary Accession #

Q14164

UniProt Secondary Accession #

Q3B754; Q3KR43; Q5JTS6; D3DT78

UniProt Related Accession #

Q14164

NCBI Official Full Name

Inhibitor of nuclear factor kappa-B kinase subunit epsilon

NCBI Official Synonym Full Names

inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon

NCBI Official Symbol

IKBKE

NCBI Official Synonym Symbols

IKKE; IKKI; IKK-E; IKK-i

UniProt Protein Name

Inhibitor of nuclear factor kappa-B kinase subunit epsilon

UniProt Synonym Protein Names

Inducible I kappa-B kinase; IKK-i

Protein Family

Inhibitor of nuclear factor kappa-B kinase

UniProt Gene Name

IKBKE

UniProt Synonym Gene Names

IKKE; IKKI; KIAA0151; I-kappa-B kinase epsilon; IKK-E; IKK-epsilon; IkBKE; IKK-i

UniProt Entry Name

IKKE_HUMAN

NCBI Protein Information

inhibitor of nuclear factor kappa-B kinase subunit epsilon; IKK-epsilon; I-kappa-B kinase epsilon; inducible IkappaB kinase; IKK-related kinase epsilon; inducible I kappa-B kinase

NCBI Summary for IKBKE

IKBKE is a noncanonical I-kappa-B (see MIM 164008) kinase (IKK) that is essential for regulating antiviral signaling pathways. IKBKE has also been identified as a breast cancer (MIM 114480) oncogene and is amplified and overexpressed in over 30% of breast carcinomas and breast cancer cell lines (Hutti et al., 2009 [PubMed 19481526]).[supplied by OMIM, Oct 2009]

UniProt Comments for IKBKE

IKKE: a oncogenic kinase of the IKK family. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Plays a pivotal role in coordinating the activation of IRF3 and NF-kappaB in the innate immune response. Its redistribution to PML nuclear bodies upon DNA damage is TOPORS-dependent. Is a breast cancer oncogene that exerts its oncogenic properties through cRel.

Protein type: Inhibitor; Kinase, protein; EC 2.7.11.10; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Other group; IKK family

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: nucleoplasm; PML body; cytoplasm; mitochondrial membrane; endosome membrane; nucleus; cytosol

Molecular Function: NF-kappaB-inducing kinase activity; protein binding; IkappaB kinase activity; ATP binding

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; DNA damage response, signal transduction resulting in induction of apoptosis; MyD88-independent toll-like receptor signaling pathway; toll-like receptor signaling pathway; innate immune response; immune response; toll-like receptor 3 signaling pathway; I-kappaB phosphorylation; negative regulation of interferon type I production; toll-like receptor 4 signaling pathway; protein amino acid phosphorylation

Research Articles on IKBKE

1. in this work, we identified TPCA-1 as a direct dual inhibitor for both IKKs and STAT3, whereas treatment targeting EGFR only could not sufficiently repress NF-kappaB and STAT3 pathways for lung cancers harboring mutant EGFR.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-IKBKE antibody

Reference Product	PubMed Publications
TBK1 antibody	>33 publications with IKBKE and TBK1
IRF3 antibody	>28 publications with IKBKE and IRF3
TANK antibody	>21 publications with IKBKE and TANK
TNFAIP3 antibody	>11 publications with IKBKE and TNFAIP3
IRF7 antibody	>6 publications with IKBKE and IRF7
UBC antibody	>4 publications with IKBKE and UBC
TAX1BP1 antibody	>3 publications with IKBKE and TAX1BP1
AZI2 antibody	>2 publications with IKBKE and AZI2
MAVS antibody	>1 publications with IKBKE and MAVS

Pathways associated with anti-IKBKE antibody

Products by Pathway	Pathway Diagram
Activated TLR4 Signalling Pathway antibodies	Activated TLR4 Signalling Pathway Diagram
Activation Of IRF3/IRF7 Mediated By TBK1/IKK Epsilon Pathway antibodies	Activation Of IRF3/IRF7 Mediated By TBK1/IKK Epsilon Pathway Diagram
Cytosolic DNA-sensing Pathway antibodies	Cytosolic DNA-sensing Pathway Diagram
Cytosolic DNA-sensing Pathway antibodies	Cytosolic DNA-sensing Pathway Diagram
Hepatitis B Pathway antibodies	Hepatitis B Pathway Diagram
Hepatitis C Pathway antibodies	Hepatitis C Pathway Diagram
Hepatitis C Pathway antibodies	Hepatitis C Pathway Diagram
Herpes Simplex Infection Pathway antibodies	Herpes Simplex Infection Pathway Diagram
Herpes Simplex Infection Pathway antibodies	Herpes Simplex Infection Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram

Diseases associated with anti-IKBKE antibody

Disease Name	Pubmed Publications
Necrosis Antibodies	>438 publications with IKBKE and Necrosis
Inflammation Antibodies	>190 publications with IKBKE and Inflammation
Congenital Abnormalities Antibodies	>40 publications with IKBKE and Congenital Abnormalities
Cell Transformation, Neoplastic Antibodies	>28 publications with IKBKE and Cell Transformation, Neoplastic
Disease Models, Animal Antibodies	>26 publications with IKBKE and Disease Models, Animal
Lymphoma, Non-Hodgkin Antibodies	>17 publications with IKBKE and Lymphoma, Non-Hodgkin
Atherosclerosis Antibodies	>15 publications with IKBKE and Atherosclerosis
Liver Diseases Antibodies	>13 publications with IKBKE and Liver Diseases
Adenocarcinoma Antibodies	>13 publications with IKBKE and Adenocarcinoma
Nervous System Diseases Antibodies	>12 publications with IKBKE and Nervous System Diseases

Organs/Tissues associated with anti-IKBKE antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>180 publications with IKBKE and Blood
Vascular Antibodies	>58 publications with IKBKE and Vascular
Lung Antibodies	>55 publications with IKBKE and Lung
Skin Antibodies	>46 publications with IKBKE and Skin
Embryonic Tissue Antibodies	>33 publications with IKBKE and Embryonic Tissue
Bone Antibodies	>32 publications with IKBKE and Bone
Kidney Antibodies	>30 publications with IKBKE and Kidney
Muscle Antibodies	>27 publications with IKBKE and Muscle
Intestine Antibodies	>21 publications with IKBKE and Intestine
Heart Antibodies	>19 publications with IKBKE and Heart