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IL12A Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6483009

MBS6483009 is an antibody targeting IL12A. Also known as IL12A, CT (NK Cell Stimulatory Factor Chain 1, NKSF1, Cytotoxic Lymphocyte Maturation Factor 35kD Subunit, CLMF p35, IL-12 Subunit p35, Interleukin-12 Subunit alpha, IL-12A) (FITC). Reactive in Human.… More details

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Product Overview

Online Datasheet in PDF
Product Name
IL12A, Polyclonal Antibody
Full Product Name
IL12A, CT (NK Cell Stimulatory Factor Chain 1, NKSF1, Cytotoxic Lymphocyte Maturation Factor 35kD Subunit, CLMF p35, IL-12 Subunit p35, Interleukin-12 Subunit alpha, IL-12A) (FITC)
Product Gene Name
anti-IL12A antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Purity/Purification
Purified by Immunoaffinity chromatography.
Specificity
Recognizes human IL12A.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
24,874 Da
OMIM
161560
3D Structure
ModBase 3D Structure for P29459
Immunogen
Synthetic peptide selected from the C-terminal region of human IL12A (KLH).
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-IL12A antibody

Antibodies; Interleukin
Applications Tested/Suitable for anti-IL12A antibody
Western Blot (WB), FLISA
Application Notes for anti-IL12A antibody
WB: 1:100-1:500
FLISA: 1:1,000
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-IL12A antibody

Western blot analysis of IL12A Antibody (C-term) in MDA-MB231 cell line lysates (35ug/lane). IL12A (arrow) was detected using the purified Pab.
Rabbit Anti-IL12A Polyclonal Antibody – Cat# MBS6483009

Immunohistochemistry (IHC) of anti-IL12A antibody

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with IL12A Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-IL12A Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6483009

Flow Cytometry (FC/FACS) of anti-IL12A antibody

IL12A Antibody (C-term) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-IL12A Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6483009

Product References and Citations for anti-IL12A antibody

1. D'Andrea, A., et.al., J. Exp. Med. 176(5), 1387-1398 (1992).

Other Notes

Small volumes of anti-IL12A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for IL12A. It may not necessarily be applicable to this product.

NCBI GI #
24430219
NCBI GeneID
3592
NCBI Accession #
NP_000873.2
UniProt Primary Accession #
P29459
UniProt Secondary Accession #
Q96QZ1
UniProt Related Accession #
P29459
GenBank Nucleotide #
NM_000882.3
NCBI Official Full Name
interleukin-12 subunit alpha
NCBI Official Synonym Full Names
interleukin 12A
NCBI Official Symbol
IL12A
NCBI Official Synonym Symbols
P35; CLMF; NFSK; NKSF1; IL-12A
UniProt Protein Name
Interleukin-12 subunit alpha
UniProt Synonym Protein Names
Cytotoxic lymphocyte maturation factor 35 kDa subunit; CLMF p35; IL-12 subunit p35; NK cell stimulatory factor chain 1
Protein Family
Interleukin
UniProt Gene Name
IL12A
UniProt Synonym Gene Names
NKSF1; IL-12A; CLMF p35; NKSF1
UniProt Entry Name
IL12A_HUMAN
NCBI Protein Information
interleukin-12 subunit alpha; CLMF p35; IL35 subunit; IL-12, subunit p35; interleukin 12, p35; interleukin-12 alpha chain; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; cytotoxic lymphocyte maturation factor 1, p35; cytotoxic lym

NCBI Summary for IL12A

This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008]
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UniProt Comments for IL12A

IL12A: Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine- activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC. Belongs to the IL-6 superfamily.

Protein type: Secreted, signal peptide; Secreted; Cytokine

Chromosomal Location of Human Ortholog: 3q25.33

Cellular Component: extracellular space; interleukin-12 complex; cytoplasm

Molecular Function: protein binding; interleukin-27 binding; growth factor activity; interleukin-12 beta subunit binding; protein heterodimerization activity; cytokine activity; interleukin-12 receptor binding

Biological Process: positive regulation of NK T cell activation; cell migration; positive regulation of cell adhesion; positive regulation of T cell mediated cytotoxicity; negative regulation of smooth muscle cell proliferation; positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target; response to virus; positive regulation of tyrosine phosphorylation of Stat4 protein; response to lipopolysaccharide; defense response to protozoan; positive regulation of natural killer cell mediated cytotoxicity; positive regulation of natural killer cell activation; response to UV-B; positive regulation of interferon-gamma production; defense response to Gram-positive bacterium; negative regulation of interleukin-17 production; positive regulation of T cell differentiation; positive regulation of mononuclear cell proliferation; positive regulation of lymphocyte proliferation; positive regulation of T cell proliferation; immune response; cell cycle arrest
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Research Articles on IL12A

1. circulating IL-35 in PDAC patients significantly increased, suggesting that regulating the expression of IL-35 may provide a new possible target for the treatment of PDAC patients, especially for the resectable ones.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-IL12A antibody

 Reference Product  PubMed Publications
 IL12B antibody  >47 publications with IL12A and IL12B
 IL4 antibody  >18 publications with IL12A and IL4
 IFNG antibody  >15 publications with IL12A and IFNG
 IL12RB2 antibody  >12 publications with IL12A and IL12RB2
 IL1B antibody  >11 publications with IL12A and IL1B
 IL12RB1 antibody  >10 publications with IL12A and IL12RB1
 STAT1 antibody  >5 publications with IL12A and STAT1
 JAK2 antibody  >1 publications with IL12A and JAK2

Pathways associated with anti-IL12A antibody

 Products by Pathway  Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram

Diseases associated with anti-IL12A antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >35 publications with IL12A and Neoplasms
 Inflammation Antibodies  >18 publications with IL12A and Inflammation
 Necrosis Antibodies  >16 publications with IL12A and Necrosis
 Liver Diseases Antibodies  >11 publications with IL12A and Liver Diseases
 Disease Models, Animal Antibodies  >10 publications with IL12A and Disease Models, Animal
 Multiple Sclerosis Antibodies  >10 publications with IL12A and Multiple Sclerosis
 Hepatitis Antibodies  >7 publications with IL12A and Hepatitis
 Brain Diseases Antibodies  >7 publications with IL12A and Brain Diseases
 Liver Cirrhosis, Biliary Antibodies  >7 publications with IL12A and Liver Cirrhosis, Biliary
 Neoplasms, Experimental Antibodies  >5 publications with IL12A and Neoplasms, Experimental

Organs/Tissues associated with anti-IL12A antibody

 Organ/Tissue Name  Pubmed Publications
 Spleen Antibodies  >5 publications with IL12A and Spleen
 Uterus Antibodies  >3 publications with IL12A and Uterus
 Brain Antibodies  >3 publications with IL12A and Brain
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