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IL12RB2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6309871

The protein encoded by this gene is a type I transmembrane protein identified as a subunit of the interleukin 12 receptor complex. The coexpression of this and IL12RB1 proteins was shown to lead to the formation of high-affinity IL12 binding sites and… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
IL12RB2, Polyclonal Antibody
Full Product Name
IL12RB2, CT (IL12RB2, Interleukin-12 receptor subunit beta-2) (AP)
Product Gene Name
Product Synonym Names
IL12RB2; Interleukin-12 receptor subunit beta-2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human IL12RB2.
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
75 kDa (predicted detection band MW)
OMIM
601642
3D Structure
ModBase 3D Structure for Q99665
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 756-783 in the C-terminal region of human IL12RB2 (Q99665).
Conjugate
AP
Note
Preservative Free

Product Categories/Family for anti-IL12RB2 antibody

Applications Tested/Suitable for anti-IL12RB2 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes for anti-IL12RB2 antibody
IHC: 1:50-100
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-IL12RB2 antibody

WesternBlot analysis in MDA-MB435 cell line lysates (35ug/lane) usingMBS6010512. This demonstrates that MBS6010512 detected the IL12RB2 protein (arrow).
Rabbit Anti-IL12RB2 Polyclonal Antibody – Cat# MBS6309871

Immunohistochemistry (IHC) of anti-IL12RB2 antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsils tissue using MBS6010512 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6010512 for immunohistochemistry.
Rabbit Anti-IL12RB2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6309871

Flow Cytometry (FC/FACS) of anti-IL12RB2 antibody

Flow Cytometric analysis of MDA-MB435 cells (right histogram) using MBS6010512 compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-IL12RB2 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6309871

Product References and Citations for anti-IL12RB2 antibody

1. Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010). 2. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). 3. Liu, X., et al. Nat. Genet. 42(8):658-660(2010). 4. Mizuki, N., et al. Nat. Genet. 42(8):703-706(2010). 5. Remmers, E.F., et al. Nat. Genet. 42(8):698-702(2010).

Other Notes

Small volumes of anti-IL12RB2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL12RB2 purchase

MBS6309871 is an antibody targeting IL12RB2. Also known as IL12RB2, CT (IL12RB2, Interleukin-12 receptor subunit beta-2) (AP). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for IL12RB2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
interleukin-12 receptor subunit beta-2 isoform a
NCBI Official Synonym Full Names
interleukin 12 receptor, beta 2
NCBI Official Symbol
IL12RB2
UniProt Protein Name
Interleukin-12 receptor subunit beta-2
UniProt Gene Name
IL12RB2
UniProt Synonym Gene Names
IL-12 receptor subunit beta-2; IL-12R subunit beta-2; IL-12R-beta-2; IL-12RB2
UniProt Entry Name
I12R2_HUMAN
NCBI Protein Information
interleukin-12 receptor subunit beta-2

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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