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IL1B Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6482425

MBS6482425 is an antibody targeting IL1B. Also known as IL1B, ID (Interleukin-1 beta, IL-1 beta, Catabolin, IL1F2) (APC). Reactive in Human. Host: Rabbit. More details

$1120 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
IL1B, Polyclonal Antibody
Full Product Name
IL1B, ID (Interleukin-1 beta, IL-1 beta, Catabolin, IL1F2) (APC)
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified by Protein A and Peptide Affinity Chromatography.
Specificity
Recognizes human IL1B.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
Calculated: 30 kDa; Observed: 36 kDa
Chromosome Location
Chromosome: 2; NC_000002.11 (113587337..113594356, complement). Location: 2q14
OMIM
147720
3D Structure
ModBase 3D Structure for P01584
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 148-174 in the central region of human IL1B, UniProt Accession #NP_000567.1; P01584. Species sequence homology: rabbit and monkey
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-IL1B antibody

Applications Tested/Suitable for anti-IL1B antibody
Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-IL1B antibody
FC/FACS: 1:10-1:50
FLISA: 1:1000
IHC: 1:50-1:100
WB: 1:100-1:500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-IL1B antibody

Western Blot analysis of IL1B in NCI-H460 cell line lysate (35ug/lane) usingMBS630428.
Rabbit Anti-IL1B Polyclonal Antibody – Cat# MBS6482425

Immunohistochemistry (IHC) of anti-IL1B antibody

Immunohistochemistry staining of formalin-fixed and paraffin-embedded human lung carcinoma usingMBS630428. Peroxidase-conjugated secondary antibody and DAB staining were used for visualization.
Rabbit Anti-IL1B Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6482425

Flow Cytometry (FC/FACS) of anti-IL1B antibody

Flow Cytometric analysis of NCI-H292 cells using MBS630428 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibody wasused for the analysis.
Rabbit Anti-IL1B Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6482425

Product References and Citations for anti-IL1B antibody

1. Yu, J., et.al., Am. J. Gastroenterol. (2009). 2. Ito, A., et.al., J. Biol. Chem. 271(25), 14657-14660 (1996).

Other Notes

Small volumes of anti-IL1B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for IL1B. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
interleukin-1 beta proprotein
NCBI Official Synonym Full Names
interleukin 1, beta
NCBI Official Symbol
IL1B
NCBI Official Synonym Symbols
IL-1; IL1F2; IL1-BETA
UniProt Protein Name
Interleukin-1 beta
UniProt Synonym Protein Names
Catabolin
Protein Family
UniProt Gene Name
IL1B
UniProt Synonym Gene Names
IL1F2; IL-1 beta
UniProt Entry Name
IL1B_HUMAN
NCBI Protein Information
interleukin-1 beta; IL-1 beta; catabolin; preinterleukin 1 beta; pro-interleukin-1-beta

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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