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IL32 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750825

Description: Interleukin 32 (Il32) is a protein that in humans is encoded by the IL32 gene. This gene encodes a member of the cytokine family. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
IL32, Polyclonal Antibody
Full Product Name
Anti-IL32 Picoband antibody
Product Gene Name
anti-IL32 antibody
Product Synonym Names
Interleukin-32; IL-32; Natural killer cells protein 4; Tumor necrosis factor alpha-inducing factor; IL32; NK4; TAIF
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
No cross reactivity with other proteins.
Form/Format
Lyophilized
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
17,003 Da
OMIM
M59807 mRNA
3D Structure
ModBase 3D Structure for P24001
Immunogen
E Coli-derived human IL32 recombinant protein (Position: A31-K234).
Subcellular Localization
Secreted.
Tissue Specificity
Selectively expressed in lymphocytes. Expression is more prominent in immune cells than in non-immune cells.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-IL32 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-IL32 antibody
WB: 0.1-0.5mug/ml
ELISA(Cap): 1-5mug/ml

Western Blot (WB) of anti-IL32 antibody

Figure 1. Western blot analysis of IL32 using anti-IL32 antibody (MBS1750825).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human IL32 protein 1ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL32 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL32 at approximately 19KD. The expected band size for IL32 is at 15KD.
Rabbit Anti-IL32 Polyclonal Antibody – Cat# MBS1750825

Other Notes

Small volumes of anti-IL32 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL32 purchase

MBS1750825 is an antibody targeting IL32. Also known as Anti-IL32 Picoband antibody. Reactive in Human
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for IL32. It may not necessarily be applicable to this product.

NCBI GI #
61639466
NCBI GeneID
9235
NCBI Accession #
NP_001012649.1
UniProt Primary Accession #
P24001
UniProt Secondary Accession #
Q5VFH7; Q5VFH8; Q8WV38; Q96GK9; A6NNM0; A8MPX0; B4DJM1; B8Q191; D3DUB0; D3DUB2
UniProt Related Accession #
P24001
GenBank Nucleotide #
NM_001012631.1
NCBI Official Full Name
interleukin-32 isoform B
NCBI Official Synonym Full Names
interleukin 32
NCBI Official Symbol
IL32
NCBI Official Synonym Symbols
NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; IL-32beta; IL-32alpha; IL-32delta; IL-32gamma
UniProt Protein Name
Interleukin-32
UniProt Synonym Protein Names
Natural killer cells protein 4; Tumor necrosis factor alpha-inducing factor
Protein Family
Interleukin
UniProt Gene Name
IL32
UniProt Synonym Gene Names
NK4; TAIF; IL-32
NCBI Protein Information
interleukin-32

NCBI Summary for IL32

This gene encodes a member of the cytokine family. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFalpha from macrophage cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
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UniProt Comments for IL32

Cytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF-alpha and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK.

Research Articles on IL32

1. In Kaposi sarcoma (KS) splicing ratio of the IL-32 isoforms showed IL-32gamma as the highest expressed isoform, followed by IL-32beta, in HIV-related KS cases compared with controls. Our data suggest a possible survival mechanism by the splicing of IL-32gamma to IL-32beta and also IL-6, IL-8, and CXCR1 signaling pathways to reverse the proapoptotic effect of the IL-32gamma isoform, leading to tumor cell survival and th...
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-IL32 antibody

 Reference Product  PubMed Publications
 IL18 antibody  >5 publications with IL32 and IL18
 IL1B antibody  >3 publications with IL32 and IL1B
 PTGS2 antibody  >2 publications with IL32 and PTGS2
 CXCL2 antibody  >1 publications with IL32 and CXCL2

Diseases associated with anti-IL32 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >65 publications with IL32 and Inflammation
 Necrosis Antibodies  >53 publications with IL32 and Necrosis
 Immune System Diseases Antibodies  >34 publications with IL32 and Immune System Diseases
 Lung Diseases Antibodies  >14 publications with IL32 and Lung Diseases
 Vascular Diseases Antibodies  >12 publications with IL32 and Vascular Diseases
 Carcinoma Antibodies  >11 publications with IL32 and Carcinoma
 Nervous System Diseases Antibodies  >9 publications with IL32 and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >9 publications with IL32 and Cardiovascular Diseases
 Lung Neoplasms Antibodies  >7 publications with IL32 and Lung Neoplasms
 Liver Diseases Antibodies  >7 publications with IL32 and Liver Diseases

Organs/Tissues associated with anti-IL32 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >64 publications with IL32 and Blood
 Lung Antibodies  >19 publications with IL32 and Lung
 Vascular Antibodies  >15 publications with IL32 and Vascular
 Bone Antibodies  >10 publications with IL32 and Bone
 Intestine Antibodies  >9 publications with IL32 and Intestine
 Liver Antibodies  >8 publications with IL32 and Liver
 Bone Marrow Antibodies  >7 publications with IL32 and Bone Marrow
 Stomach Antibodies  >6 publications with IL32 and Stomach
 Lymph Node Antibodies  >6 publications with IL32 and Lymph Node
 Brain Antibodies  >4 publications with IL32 and Brain
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