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IL6 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6310293

MBS6310293 is an antibody targeting IL6. Also known as IL6, ID (IL6, IFNB2, Interleukin-6, B Cell stimulatory factor 2, CTL differentiation factor, Hybridoma growth factor, Interferon beta-2) (Azide free) (HRP). Reactive in Human. Host: Rabbit. More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
IL6, Polyclonal Antibody
Full Product Name
IL6, ID (IL6, IFNB2, Interleukin-6, B Cell stimulatory factor 2, CTL differentiation factor, Hybridoma growth factor, Interferon beta-2) (Azide free) (HRP)
Product Gene Name
Product Synonym Names
IL6; IFNB2; Interleukin-6; B-cell stimulatory factor 2; CTL differentiation factor; Hybridoma growth factor; Interferon beta-2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Purity/Purification
Purified by Protein A and Peptide Affinity Chromatography.
Specificity
Recognizes human IL6.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
212
Chromosome Location
Chromosome: 7; NC_000007.13 (22766766..22771621). Location: 7p21
OMIM
phenotype 604302
3D Structure
ModBase 3D Structure for P05231
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 27-55 in the central region of human IL6, UniProt Accession #P05231.
Conjugate
HRP
Note
Preservative Free

Product Categories/Family for anti-IL6 antibody

Applications Tested/Suitable for anti-IL6 antibody
Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-IL6 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-IL6 antibody

Western Blot analysis in NCI-H460 cell line lysates (35ug/lane) using 037016. This demonstrates that 037016 detected the IL6 protein (arrow).
Rabbit Anti-IL6 Polyclonal Antibody – Cat# MBS6310293

Western Blot (WB) of anti-IL6 antibody

Western Blot analysis in F9 whole cell lysate (20ug) using 037016 (1:2000). Secondary IgG (HRP) Goat Anti-Rabbit Predicted band size: 57kD. Blocking/Dilution buffer: 5% NFDM/TBST.
Rabbit Anti-IL6 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6310293

Testing Data of anti-IL6 antibody

All lanes: 037016 (1:2000) Lane 1: Hela whole cell lysate Lane 2: F9 whole cell lysate. Lysates/proteins at 20ug/lane. Secondary IgG (HRP) Goat Anti-Rabbit Predicted band size: 57kD. Blocking/Dilution buffer: 5% NFDM/TBST.
Rabbit Anti-IL6 Polyclonal Antibody, Testing Data – Cat# MBS6310293

Immunohistochemistry (IHC) of anti-IL6 antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue using 037016 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of 037016 for immunohistochemistry.
Rabbit Anti-IL6 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6310293

Product References and Citations for anti-IL6 antibody

1. Perez, A.B., et al. Hum. Immunol. 71(11):1135-1140(2010). 2. Weng, X.M., et al. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi 24(1):42-44(2010). 3. Santhosh, S., et al. Trop Gastroenterol 31(1):30-33(2010). 4. Gein, O.N., et al. Patol Fiziol Eksp Ter 1, 10-13 (2010). 5. Tuttolomondo, A., et al. Cardiovasc Diabetol 9, 50 (2010).

Other Notes

Small volumes of anti-IL6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for IL6. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
interleukin-6
NCBI Official Synonym Full Names
interleukin 6 (interferon, beta 2)
NCBI Official Symbol
IL6
NCBI Official Synonym Symbols
HGF; HSF; BSF2; IL-6; IFNB2
UniProt Protein Name
Interleukin-6
UniProt Synonym Protein Names
B-cell stimulatory factor 2; BSF-2; CTL differentiation factor; CDF; Hybridoma growth factor; Interferon beta-2; IFN-beta-2
Protein Family
UniProt Gene Name
IL6
UniProt Synonym Gene Names
IFNB2; IL-6; BSF-2; CDF; IFN-beta-2
UniProt Entry Name
IL6_HUMAN
NCBI Protein Information
interleukin-6; CDF; BSF-2; IFN-beta-2; interferon beta-2; interleukin BSF-2; hybridoma growth factor; CTL differentiation factor; B-cell stimulatory factor 2; B-cell differentiation factor

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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