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IL8 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9607651

MBS9607651 is an antibody targeting IL8. Also known as IL8 Antibody. Reactive in Human. Host: Rabbit. More details

$325 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
IL8, Polyclonal Antibody
Full Product Name
IL8 Antibody
Product Gene Name
anti-IL8 antibody
Product Synonym Gene Name
CXCL8
Product Synonym Names
(Ala-IL-8)77; (Ser-IL-8)72; 9E3; Beta thromboglobulin like protein; C-X-C motif chemokine 8; CEF-4; chemokine, CXC motif, ligand 8; CXCL8; Emoctakin; GCP-1; GCP/IL-8 protein I; GCP/IL-8 protein II; GCP/IL-8 protein III; GCP/IL-8 protein IV; GCP/IL-8 protein V; GCP/IL-8 protein VI; GCP1; Granulocyte chemotactic protein 1; IL-8; IL-8(1-77); IL-8(9-77); IL8; IL8/NAP1 form I; IL8/NAP1 form II; IL8/NAP1 form III; IL8/NAP1 form IV; IL8/NAP1 form V; IL8/NAP1 form VI; IL8_HUMAN; Inteleukin 8; LECT; LUCT; Lymphocyte-derived neutrophil-activating factor; LYNAP; MDNCF; MDNCF-b; MDNCF-c; MONAP; Monocyte-derived neutrophil chemotactic factor; Monocyte-derived neutrophil-activating peptide; NAF; NAP-1; NAP1; Neutrophil activating peptide 1; Neutrophil activating protein 1; Neutrophil-activating factor; Neutrophil-activating protein 1; Protein 3 10C; Protein 3-10C; SCYB8; Small inducible cytokine subfamily B member 8; T cell chemotactic factor; T-cell chemotactic factor; TSG1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin.
Concentration
~1mg/ml (lot specific)
Preparation and Storage
Store at -20°C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
IL8 peptide (MBS9626364) is used for blocking the activity of IL8 antibody (MBS9607651)
Molecular Weight
11kDa
3D Structure
ModBase 3D Structure for P10145
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human IL8, corresponding to a region within the internal amino acids.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-IL8 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-IL8 antibody
WB 1:500-1:2000
IHC 1:50-1:200

Other dilutions should be determined by the end user.
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

Immunohistochemistry (IHC) of anti-IL8 antibody

MBS9607651 at 1/100 staining Human Melanoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary ab.
Rabbit Anti-IL8 Polyclonal Antibody – Cat# MBS9607651

Western Blot (WB) of anti-IL8 antibody

Western blot analysis of extracts of Mouse liver, using IL8 antibody. The lane on the left is treated with the antigen-specific peptide.
Rabbit Anti-IL8 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9607651

Product References and Citations for anti-IL8 antibody

Qin X, Qiao W, Wang Y, Li T, Li X, Gong T, Zhang ZR, Fu Y; Journal: Macromol Biosci. An Extracellular Matrix-Mimicking Hydrogel for Full Thickness Wound Healing in Diabetic Mice. Zhuoqi Liu, Xiaohong Yang, Yunlei Song, Bo Huang, Yingquan Xiao, Weifeng Zhu, Lehan Yu, Xi Liu, Daya Luo, Fusheng Wan; Journal: Preprints. Screening of Immunosuppressive Factors for Identifying Breast Cancer Malignant Phenotypes Using mRNA Microarray Datasets.

Other Notes

Small volumes of anti-IL8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for IL8. It may not necessarily be applicable to this product.

NCBI GI #
10834978
NCBI GeneID
3576
NCBI Accession #
NP_000575.1
UniProt Primary Accession #
P10145
UniProt Secondary Accession #
Q6FGF6; Q6LAE6; Q96RG6; Q9C077; Q9UCE1; Q9UCR8; Q9UCR9; Q9UCS0; B2R4L8
UniProt Related Accession #
P10145
GenBank Nucleotide #
NM_000584.3
NCBI Official Full Name
interleukin-8 isoform 1
NCBI Official Synonym Full Names
C-X-C motif chemokine ligand 8
NCBI Official Symbol
CXCL8
NCBI Official Synonym Symbols
IL8; NAF; GCP1; LECT; LUCT; NAP1; GCP-1; LYNAP; MDNCF; MONAP; NAP-1
UniProt Protein Name
Interleukin-8
UniProt Synonym Protein Names
C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; Emoctakin; Granulocyte chemotactic protein 1; GCP-1
Protein Family
G-protein coupled estrogen receptor
UniProt Gene Name
CXCL8
UniProt Synonym Gene Names
IL8; IL-8; GCP-1; MDNCF; MONAP; NAP-1; LYNAP; NAF
NCBI Protein Information
interleukin-8

NCBI Summary for IL8

The protein encoded by this gene is a member of the CXC chemokine family and is a major mediator of the inflammatory response. The encoded protein is secreted primarily by neutrophils, where it serves as a chemotactic factor by guiding the neutrophils to the site of infection. This chemokine is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other members of the CXC chemokine gene family form a gene cluster in a region of chromosome 4q. [provided by RefSeq, Aug 2017]
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UniProt Comments for IL8

IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
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Research Articles on IL8

1. IL-8 production was significantly enhanced following treatment with both IL-17A and CSE, while treatment with either IL-17A or CSE alone caused only a slight increase in IL-8 production.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-IL8 antibody

 Products by Pathway  Pathway Diagram
 ATF-2 Transcription Factor Network Pathway antibodies  ATF-2 Transcription Factor Network Pathway Diagram
 Activation Of Genes By ATF4 Pathway antibodies  Activation Of Genes By ATF4 Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies  Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chemokine Receptors Bind Chemokines Pathway antibodies  Chemokine Receptors Bind Chemokines Pathway Diagram

Diseases associated with anti-IL8 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >675 publications with IL8 and Inflammation
 Necrosis Antibodies  >395 publications with IL8 and Necrosis
 Hypersensitivity Antibodies  >66 publications with IL8 and Hypersensitivity
 Drug Toxicity Antibodies  >62 publications with IL8 and Drug Toxicity
 Adenocarcinoma Antibodies  >49 publications with IL8 and Adenocarcinoma
 Pulmonary Disease, Chronic Obstructive Antibodies  >48 publications with IL8 and Pulmonary Disease, Chronic Obstructive
 Pneumonia Antibodies  >34 publications with IL8 and Pneumonia
 Colorectal Neoplasms Antibodies  >30 publications with IL8 and Colorectal Neoplasms
 Kidney Diseases Antibodies  >27 publications with IL8 and Kidney Diseases
 Breast Neoplasms Antibodies  >26 publications with IL8 and Breast Neoplasms

Organs/Tissues associated with anti-IL8 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >669 publications with IL8 and Blood
 Lung Antibodies  >237 publications with IL8 and Lung
 Vascular Antibodies  >201 publications with IL8 and Vascular
 Intestine Antibodies  >90 publications with IL8 and Intestine
 Skin Antibodies  >81 publications with IL8 and Skin
 Liver Antibodies  >71 publications with IL8 and Liver
 Heart Antibodies  >67 publications with IL8 and Heart
 Bone Antibodies  >60 publications with IL8 and Bone
 Muscle Antibodies  >59 publications with IL8 and Muscle
 Brain Antibodies  >48 publications with IL8 and Brain
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