• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-INCENP antibody :: Rabbit anti-Human INCENP Polyclonal Antibody

Scan QR to view Datasheet
Catalog # MBS610766
Unit / Price
  0.1 mL  /  $540 +1 FREE 8GB USB
anti-INCENP antibody
Product Name

INCENP, Polyclonal Antibody

Also Known As

INCENP (Incenp, Inner Centromere Protein)

Product Synonym Names
Anti -INCENP (Incenp, Inner Centromere Protein)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 11; NC_000011.9 (61891445..61920635). Location: 11q12.3
OMIM
604411
3D Structure
ModBase 3D Structure for Q9NQS7
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Specificity
Detects endogenous levels of total INCENP protein.
Purity/Purification
Purified by peptide affinity chromatography.
Form/Format
Supplied as a liquid in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100ug/ml BSA and 50% glycerol.
Immunogen
Synthetic peptide (KLH-coupled) corresponding to amino acids surrounding Pro240 of human INCENP.
Preparation and Storage
For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of anti-INCENP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-INCENP antibody
INCENP (inner centromere protein antigens 135kD, 155kD) is a chromosomal passenger protein crucial for multiple events that mediate chromosome separation during mitosis (1). At prophase INCENP is associated with chromatin whereas during prometaphase and metaphase it translocates to the inner centromere (1). Depletion of INCENP results in aberrant chromosome alignment at the metaphase plate, incomplete chromosome separation, and disruption of proper spindle formation and cytokinesis (2). INCENP is part of the chromosomal passenger complex that also contains Aurora B, borealin and survivin (2). Aurora B and INCENP are mutually dependent on each other for proper localization (3), and in Drosophila cells and C.elegans embryos that lack INCENP or survivin, Aurora B cannot organize the kinetochores and the midbody (4,5). Phosphorylation on INCENP by CDK1 on Thr59 and Thr388 leads to the association of INCENP with Plk1, another important regulator of mitotic entry and exit (6). Interaction of INCENP with Plk1 is necessary for recruitment of Plk1 to the kinetochores, and the metaphase to anaphase transition (6). Interactions have also been reported between INCENP and heterochromatin protein 1 (HP1) (7) and -tubulin (8).
Applications Tested/Suitable for anti-INCENP antibody
Western Blot (WB), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes for anti-INCENP antibody
Suitable for use in Immunofluorescence, Flow Cytometry and Western Blot.
Dilution: Immunofluorescence: 1:400
Flow Cytometry: 1:50
Western Blot: 1:1000
NCBI/Uniprot data below describe general gene information for INCENP. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
105,429 Da[Similar Products]
NCBI Official Full Name
inner centromere protein isoform 2
NCBI Official Synonym Full Names
inner centromere protein antigens 135/155kDa
NCBI Official Symbol
INCENP  [Similar Products]
NCBI Official Synonym Symbols
FLJ31633; MGC111393
  [Similar Products]
NCBI Protein Information
inner centromere protein; OTTHUMP00000236001; OTTHUMP00000236061; chromosomal passenger protein; inner centromere protein INCENP; binds and activates aurora-B and -C in vivo and in vitro
UniProt Protein Name
Inner centromere protein
Protein Family
UniProt Gene Name
INCENP  [Similar Products]
UniProt Entry Name
INCE_HUMAN
NCBI Summary for INCENP
In mammalian cells, 2 broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger,' or transiently interacting, proteins (reviewed by Choo, 1997). The constitutive proteins include CENPA (centromere protein A; MIM 117139), CENPB (MIM 117140), CENPC1 (MIM 117141), and CENPD (MIM 117142). The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle (Earnshaw and Mackay, 1994 [PubMed 8088460]). These include CENPE (MIM 117143); MCAK (MIM 604538); KID (MIM 603213); cytoplasmic dynein (e.g., MIM 600112); CliPs (e.g., MIM 179838); and CENPF/mitosin (MIM 600236). The inner centromere proteins (INCENPs) (Earnshaw and Cooke, 1991 [PubMed 1860899]), the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where they are discarded after cytokinesis (Cutts et al., 1999 [PubMed 10369859]).[supplied by OMIM]
UniProt Comments for INCENP
INCENP: Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Probably acts through association with AURKB or AURKC. Seems to bind directly to microtubules. Controls the kinetochore localization of BUB1. Homodimer or heterodimer. Interacts with H2AFZ. Interacts with CBX3. Interacts with tubulin beta chain. Interacts with AURKB and AURKC. Component of the chromosomal passenger complex (CPC) composed of at least BIRC5/survivin, CDCA8/borealin, INCENP, AURKB and AURKC. Interacts with EVI5. Interacts with CDCA8 and BIRC5; interaction is direct. Interacts with CBX5; POGZ and INCENP compete for interaction with CBX5. Interacts with POGZ. Interacts with JTB. Belongs to the INCENP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; EC 2.7.11.1

Chromosomal Location of Human Ortholog: 11q12.3

Cellular Component: kinetochore; centric heterochromatin; lateral element; microtubule; central element; protein complex; spindle; midbody; cytosol; chromosome, pericentric region; chromocenter

Molecular Function: protein binding

Biological Process: mitosis; cytokinesis; mitotic cell cycle; chromosome segregation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000