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INSIG1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1758255

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
INSIG1, Polyclonal Antibody
Full Product Name
Anti-INSIG1 Antibody Picoband
Product Gene Name
anti-INSIG1 antibody
Product Synonym Names
Serine/threonine-protein kinase AtPK1/AtPK6; 70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70(S6K) alpha antibody, p70(S6K)-alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Specificity
Expressed in all tissues.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
Molecular Weight
29,987 Da
Chromosome Location
Chromosome: 7; NC_000007.13 (155089486..155101945). Location: 7q36
OMIM
602055
3D Structure
ModBase 3D Structure for O15503
Immunogen
E coli-derived human INSIG1 recombinant protein (Position: M1-R87).
Subcellular Localization
Cytoplasm, Nucleus
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross reactivity with other proteins.
Protein Function
Downstream effector of TOR signaling pathway involved in osmotic stress response. Could be involved in the control of plant growth and development. Phosphorylates the ribosomal proteins P14, P16 and S6. Functions as a repressor of cell proliferation and required for maintenance of chromosome stability and ploidy levels through the RBR1-E2F pathway.

Product Categories/Family for anti-INSIG1 antibody

Primary Antibodies; Signal Transduction; Signaling Pathway; Small G Proteins
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-INSIG1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-INSIG1 antibody
WB: 0.25-0.5ug/ml, Human
IHC: 2-5ug/ml, Human
ICC/IF: 5ug/ml, Human
FC/FACS (Fixed): 1-3ug/1x106 cells, Human
ELISA: 0.1-0.5ug/ml

Western Blot (WB) of anti-INSIG1 antibody

Figure 1. Western blot analysis of INSIG1 using anti-INSIG1 antibody (MBS1758255).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human HUH7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INSIG1 antigen affinity purified polyclonal antibody (#MBS1758255) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for INSIG1 at approximately 66 kDa. The expected band size for INSIG1 is at 30 kDa.
Rabbit Anti-INSIG1 Polyclonal Antibody – Cat# MBS1758255

Immunohistochemistry (IHC) of anti-INSIG1 antibody

Figure 2. IHC analysis of INSIG1 using anti-INSIG1 antibody (MBS1758255).
INSIG1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-INSIG1 Antibody (MBS1758255) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (#MBS176453) with DAB as the chromogen.
Rabbit Anti-INSIG1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1758255

Immunofluorescence (IF) of anti-INSIG1 antibody

Figure 3. IF analysis of INSIG1 using anti-INSIG1 antibody (MBS1758255).
INSIG1 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (#MBS176582) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-INSIG1 Antibody (MBS1758255) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Rabbit Anti-INSIG1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS1758255

Flow Cytometry (FC/FACS) of anti-INSIG1 antibody

Figure 4. Flow Cytometry analysis of MCF-7 cells using anti-INSIG1 antibody (MBS1758255).
Overlay histogram showing MCF-7 cells stained with MBS1758255 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-INSIG1 Antibody (MBS1758255, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (MBS177405, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Rabbit Anti-INSIG1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1758255

Product References and Citations for anti-INSIG1 antibody

1. Diamond, R. H., Du, K., Lee, V. M., Mohn, K. L., Haber, B. A., Tewari, D. S., Taub, R. Novel delayed-early and highly insulin-induced growth response genes: identification of HRS, a potential regulator of alternative pre-mRNA splicing. J. Biol. Chem. 268: 15185-15192, 1993.2. Engelking, L. J., Liang, G., Hammer, R. E., Takaishi, K., Kuriyama, H., Evers, B. M., Li, W.-P., Horton, J. D., Goldstein, J. L., Brown, M. S. Schoenheimer effect explained--feedback regulation of cholesterol synthesis in mice mediated by Insig proteins. J. Clin. Invest. 115: 2489-2498, 2005.3. Gong, Y., Lee, J. N., Brown, M. S., Goldstein, J. L., Ye, J. Juxtamembranous aspartic acid in Insig-1 and Insig-2 is required for cholesterol homeostasis. Proc. Nat. Acad. Sci. 103: 6154-6159, 2006.

Other Notes

Small volumes of anti-INSIG1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for INSIG1 purchase

MBS1758255 is an antibody targeting INSIG1. Also known as Anti-INSIG1 Antibody Picoband. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for INSIG1. It may not necessarily be applicable to this product.

NCBI GI #
241982794
NCBI GeneID
3638
NCBI Accession #
NP_938150.2
UniProt Primary Accession #
O15503
UniProt Secondary Accession #
Q53XW8; Q9BUV5; A8K6L0
UniProt Related Accession #
O15503
GenBank Nucleotide #
NM_198336.2
NCBI Official Full Name
insulin-induced gene 1 protein isoform 2
NCBI Official Synonym Full Names
insulin induced gene 1
NCBI Official Symbol
INSIG1
NCBI Official Synonym Symbols
CL6; CL-6; MGC1405
UniProt Protein Name
Insulin-induced gene 1 protein
Protein Family
Insulin-induced gene 1 protein
UniProt Gene Name
INSIG1
UniProt Entry Name
INSI1_HUMAN
NCBI Protein Information
insulin-induced gene 1 protein; OTTHUMP00000216469; OTTHUMP00000216470; OTTHUMP00000216471; INSIG-1 membrane protein

NCBI Summary for INSIG1

Oxysterols regulate cholesterol homeostasis through the liver X receptor (LXR)- and sterol regulatory element-binding protein (SREBP)-mediated signaling pathways. This gene is an insulin-induced gene. It encodes an endoplasmic reticulum (ER) membrane protein that plays a critical role in regulating cholesterol concentrations in cells. This protein binds to the sterol-sensing domains of SREBP cleavage-activating protein (SCAP) and HMG CoA reductase, and is essential for the sterol-mediated trafficking of the two proteins. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]
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UniProt Comments for INSIG1

INSIG1: Mediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. Functions by blocking the processing of sterol regulatory element-binding proteins (SREBPs). Capable of retaining the SCAP-SREBF2 complex in the ER thus preventing it from escorting SREBPs to the Golgi. Initiates the sterol-mediated ubiquitin-mediated endoplasmic reticulum-associated degradation (ERAD) of HMGCR via recruitment of the reductase to the ubiquitin ligase, AMFR/gp78. May play a role in growth and differentiation of tissues involved in metabolic control. May play a regulatory role during G0/G1 transition of cell growth. Belongs to the INSIG family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 7q36

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum

Molecular Function: protein binding

Biological Process: cell proliferation; inner ear morphogenesis; triacylglycerol metabolic process; metabolic process; middle ear morphogenesis; palate development; negative regulation of fat cell differentiation; SREBP-mediated signaling pathway; cholesterol biosynthetic process; negative regulation of fatty acid biosynthetic process
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Research Articles on INSIG1

1. Observational study of gene-disease association, gene-gene interaction, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-INSIG1 antibody

 Reference Product  PubMed Publications
 SCAP antibody  >27 publications with INSIG1 and SCAP
 SREBF2 antibody  >9 publications with INSIG1 and SREBF2
 HMGCR antibody  >3 publications with INSIG1 and HMGCR
 PGRMC1 antibody  >1 publications with INSIG1 and PGRMC1

Diseases associated with anti-INSIG1 antibody

 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >13 publications with INSIG1 and Liver Diseases
 Fatty Liver Antibodies  >13 publications with INSIG1 and Fatty Liver
 Obesity Antibodies  >12 publications with INSIG1 and Obesity
 Neoplasms Antibodies  >9 publications with INSIG1 and Neoplasms
 Cardiovascular Diseases Antibodies  >8 publications with INSIG1 and Cardiovascular Diseases
 Atherosclerosis Antibodies  >7 publications with INSIG1 and Atherosclerosis
 Insulin Resistance Antibodies  >6 publications with INSIG1 and Insulin Resistance
 Hypercholesterolemia Antibodies  >5 publications with INSIG1 and Hypercholesterolemia
 Vascular Diseases Antibodies  >5 publications with INSIG1 and Vascular Diseases
 Carcinoma Antibodies  >5 publications with INSIG1 and Carcinoma

Organs/Tissues associated with anti-INSIG1 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >29 publications with INSIG1 and Liver
 Blood Antibodies  >15 publications with INSIG1 and Blood
 Adipose Tissue Antibodies  >5 publications with INSIG1 and Adipose Tissue
 Ovary Antibodies  >5 publications with INSIG1 and Ovary
 Heart Antibodies  >4 publications with INSIG1 and Heart
 Mammary Gland Antibodies  >4 publications with INSIG1 and Mammary Gland
 Muscle Antibodies  >4 publications with INSIG1 and Muscle
 Brain Antibodies  >4 publications with INSIG1 and Brain
 Connective Tissue Antibodies  >3 publications with INSIG1 and Connective Tissue
 Kidney Antibodies  >2 publications with INSIG1 and Kidney
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