Rabbit Anti-ITGA4 Polyclonal Antibody – Cat# MBS1758078

Figure 1. Western blot analysis of Integrin Alpha 4/ITGA4 using anti-Integrin Alpha 4/ITGA4 antibody (MBS1758078).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human THP-1 whole cell lysates,
Lane 3: human HL-60 whole cell lysates,
Lane 4: human U87 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin Alpha 4/ITGA4 antigen affinity purified polyclonal antibody (#MBS1758078) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for Integrin Alpha 4/ITGA4 at approximately 140-150 kDa. The expected band size for Integrin Alpha 4/ITGA4 is at 115 kDa.

Integrin Alpha 4/ITGA4 (ITGA4) Antibody – Rabbit Polyclonal

Name: ITGA4 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1758078
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1758078

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Integrin Alpha 4/ITGA4, Polyclonal Antibody

Full Product Name

Anti-Integrin Alpha 4/ITGA4 Antibody Picoband

Product Gene Name

anti-ITGA4 antibody

Product Synonym Names

Serine/threonine-protein kinase AtPK1/AtPK6; 70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70(S6K) alpha antibody, p70(S6K)-alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purity/Purification

Immunogen affinity purified.

Specificity

Expressed in all tissues.

Preparation and Storage

At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.

Molecular Weight

1032

OMIM

192975

3D Structure

ModBase 3D Structure for P13612

Immunogen

E coli-derived human Integrin Alpha 4/ITGA4 recombinant protein (Position: N35-V269). Human ITGA4 shares 85.5% amino acid (aa) sequence identity with mouse ITGA4.

Subcellular Localization

Cytoplasm, Nucleus

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.

Cross Reactivity

No cross reactivity with other proteins.

Protein Function

Downstream effector of TOR signaling pathway involved in osmotic stress response. Could be involved in the control of plant growth and development. Phosphorylates the ribosomal proteins P14, P16 and S6. Functions as a repressor of cell proliferation and required for maintenance of chromosome stability and ploidy levels through the RBR1-E2F pathway.

Product Categories/Family for anti-ITGA4 antibody

Primary Antibodies; Signal Transduction; Signaling Pathway; Small G Proteins

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-ITGA4 antibody

Western Blot (WB), ELISA (EIA)

Application Notes for anti-ITGA4 antibody

WB: 0.25-0.5ug/ml, Human
ELISA: 0.1-0.5ug/ml

Product References and Citations for anti-ITGA4 antibody

1. Jaspers, M., Zhang, Z., Marynen, P., Vekemans, S., Aly, M. S., Cuppens, H., Hillicker, C., Cassiman, J.-J.Localization of the genes encoding the alpha-2 and alpha-4 subunits of the human VLA-receptors to chromosome 5q23-31 and 2q31-32 respectively. (Abstract)Cytogenet. Cell Genet. 58: 1870 only, 1991.2. Miller, D. H., Khan, O. A., Sheremata, W. A., Blumhardt, L. D., Rice, G. P. A., Libonati, M. A., Willmer-Hulme, A. J., Dalton, C. M., Miszkiel, K. A., O'Connor, P. W.A controlled trial of natalizumab for relapsing multiple sclerosis.New Eng. J. Med. 348: 15-23, 2003.

Other Notes

Small volumes of anti-ITGA4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ITGA4 purchase

MBS1758078 is an antibody targeting ITGA4. Also known as Anti-Integrin Alpha 4/ITGA4 Antibody Picoband. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ITGA4. It may not necessarily be applicable to this product.

NCBI GI #

311033436

NCBI GeneID

3676

NCBI Accession #

P13612.3

UniProt Primary Accession #

P13612

UniProt Secondary Accession #

Q7Z4L6; D3DPG4

UniProt Related Accession #

P13612; AAB25486

NCBI Official Full Name

Integrin alpha-4

NCBI Official Synonym Full Names

integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)

NCBI Official Symbol

ITGA4

NCBI Official Synonym Symbols

IA4; CD49D

UniProt Protein Name

Integrin alpha-4

UniProt Synonym Protein Names

CD49 antigen-like family member D; Integrin alpha-IV; VLA-4 subunit alpha; CD_antigen: CD49d

Protein Family

Integrin

UniProt Gene Name

ITGA4

UniProt Synonym Gene Names

CD49D

UniProt Entry Name

ITA4_HUMAN

NCBI Protein Information

integrin alpha-4; 269C wild type; integrin alpha 4; integrin alpha-IV; VLA-4 subunit alpha; integrin alpha-4 subunit; CD49 antigen-like family member D; antigen CD49D, alpha-4 subunit of VLA-4 receptor; very late activation protein 4 receptor, alpha 4 sub

NCBI Summary for ITGA4

The product of this gene belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes an alpha 4 chain. Unlike other integrin alpha chains, alpha 4 neither contains an I-domain, nor undergoes disulfide-linked cleavage. Alpha 4 chain associates with either beta 1 chain or beta 7 chain. [provided by RefSeq, Jul 2008]

UniProt Comments for ITGA4

ITGA4: Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha- 4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha- 4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. Heterodimer of an alpha and a beta subunit. The alpha subunit can sometimes be cleaved into two non-covalently associated fragments. Alpha-4 associates with either beta-1 or beta-7. Alpha-4 interacts with PXN, LPXN, and TGFB1I1/HIC5. Interacts with CSPG4 through CSPG4 chondroitin sulfate glycosaminoglycan. Belongs to the integrin alpha chain family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion; Receptor, misc.

Chromosomal Location of Human Ortholog: 2q31.3

Cellular Component: focal adhesion; cell surface; membrane; plasma membrane; intercellular junction; external side of plasma membrane

Molecular Function: protein binding; fibronectin binding; metal ion binding; cell adhesion molecule binding

Biological Process: integrin-mediated signaling pathway; regulation of immune response; extracellular matrix organization and biogenesis; heart development; cell-matrix adhesion; receptor clustering; heterophilic cell adhesion; heterotypic cell-cell adhesion; leukocyte adhesion; B cell differentiation; blood vessel remodeling; blood coagulation; leukocyte tethering or rolling; leukocyte migration

Research Articles on ITGA4

1. lymphocyte trafficking into the CNS under VLA-4 blockade can occur by using the alternative adhesion molecules, PSGL-1 and MCAM, the latter representing an exclusive pathway for TH17 cells to migrate over the blood-brain barrier

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ITGA4 antibody

Reference Product	PubMed Publications
MADCAM1 antibody	>49 publications with ITGA4 and MADCAM1
PXN antibody	>15 publications with ITGA4 and PXN
ITGB1 antibody	>12 publications with ITGA4 and ITGB1
VCAM1 antibody	>10 publications with ITGA4 and VCAM1
ITGB7 antibody	>3 publications with ITGA4 and ITGB7
CD81 antibody	>2 publications with ITGA4 and CD81
FN1 antibody	>2 publications with ITGA4 and FN1
ADAM28 antibody	>1 publications with ITGA4 and ADAM28

Pathways associated with anti-ITGA4 antibody

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Arrhythmogenic Right Ventricular Cardiomyopathy Pathway antibodies	Arrhythmogenic Right Ventricular Cardiomyopathy Pathway Diagram
Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies	Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies	Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cell Surface Interactions At The Vascular Wall Pathway antibodies	Cell Surface Interactions At The Vascular Wall Pathway Diagram
Dilated Cardiomyopathy Pathway antibodies	Dilated Cardiomyopathy Pathway Diagram
Dilated Cardiomyopathy Pathway antibodies	Dilated Cardiomyopathy Pathway Diagram
ECM-receptor Interaction Pathway antibodies	ECM-receptor Interaction Pathway Diagram

Diseases associated with anti-ITGA4 antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>244 publications with ITGA4 and Inflammation
Demyelinating Diseases Antibodies	>183 publications with ITGA4 and Demyelinating Diseases
Disease Models, Animal Antibodies	>132 publications with ITGA4 and Disease Models, Animal
Central Nervous System Diseases Antibodies	>121 publications with ITGA4 and Central Nervous System Diseases
Necrosis Antibodies	>119 publications with ITGA4 and Necrosis
Lung Diseases Antibodies	>56 publications with ITGA4 and Lung Diseases
Leukemia, Lymphocytic, Chronic, B-Cell Antibodies	>34 publications with ITGA4 and Leukemia, Lymphocytic, Chronic, B-Cell
Atherosclerosis Antibodies	>21 publications with ITGA4 and Atherosclerosis
Lung Neoplasms Antibodies	>19 publications with ITGA4 and Lung Neoplasms
Liver Diseases Antibodies	>18 publications with ITGA4 and Liver Diseases

Organs/Tissues associated with anti-ITGA4 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>843 publications with ITGA4 and Blood
Vascular Antibodies	>475 publications with ITGA4 and Vascular
Bone Antibodies	>195 publications with ITGA4 and Bone
Bone Marrow Antibodies	>169 publications with ITGA4 and Bone Marrow
Brain Antibodies	>124 publications with ITGA4 and Brain
Lung Antibodies	>103 publications with ITGA4 and Lung
Embryonic Tissue Antibodies	>89 publications with ITGA4 and Embryonic Tissue
Lymph Antibodies	>84 publications with ITGA4 and Lymph
Lymph Node Antibodies	>84 publications with ITGA4 and Lymph Node
Muscle Antibodies	>76 publications with ITGA4 and Muscle