• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

IRG1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9608302

MBS9608302 is an antibody targeting IRG1. Also known as IRG1 Antibody. Reactive in Human. Host: Rabbit. More details

$325 / 0.1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
IRG1, Polyclonal Antibody
Full Product Name
IRG1 Antibody
Product Gene Name
anti-IRG1 antibody
Product Synonym Gene Name
ACOD1
Product Synonym Names
ACOD1; Aconitate decarboxylase 1; Aconitate decarboxylase; CAD; Cis aconitate decarboxylase; Immune responsive gene 1 protein homolog; Immune-responsive gene 1 protein homolog; Immunoresponsive 1 homolog (mouse); Irg1; IRG1_HUMAN; OC116; OPTB1; TCTIRC7
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
IRG1 peptide (MBS9627014) is used for blocking the activity of IRG1 antibody (MBS9608302)
Molecular Weight
55KD
3D Structure
ModBase 3D Structure for A6NK06
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human IRG1, corresponding to a region within C-terminal amino acids.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-IRG1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-IRG1 antibody
WB 1:1000-3000, IF/ICC 1:100-1:500

The optimal dilutions should be determined by the end user.

IMPORTANT:
For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween20 at 4 degree C with gentle shaking, overnight.

Immunofluorescence (IF) of anti-IRG1 antibody

MBS9608302 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(MBS9608302 1:200) and mouse antibeta tubulin Ab(MBS9610328 1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.
Rabbit Anti-IRG1 Polyclonal Antibody – Cat# MBS9608302

Western Blot (WB) of anti-IRG1 antibody

Western blot analysis of extracts from Hela cells, using IRG1 Ab. The lane on the left was treated with blocking peptide
Rabbit Anti-IRG1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9608302

Other Notes

Small volumes of anti-IRG1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for IRG1. It may not necessarily be applicable to this product.

NCBI GI #
385719224
NCBI GeneID
730249
NCBI Accession #
NP_001245335.1
UniProt Primary Accession #
A6NK06
UniProt Related Accession #
A6NK06
GenBank Nucleotide #
NM_001258406.1
NCBI Official Full Name
cis-aconitate decarboxylase
NCBI Official Synonym Full Names
aconitate decarboxylase 1
NCBI Official Symbol
ACOD1
NCBI Official Synonym Symbols
CAD; IRG1
UniProt Protein Name
Cis-aconitate decarboxylase
UniProt Synonym Protein Names
Aconitate decarboxylase; Aconitate decarboxylase 1
UniProt Gene Name
ACOD1
UniProt Synonym Gene Names
IRG1; CAD
NCBI Protein Information
cis-aconitate decarboxylase

UniProt Comments for IRG1

Involved in the inhibition of the inflammatory response. Acts as a negative regulator of the Toll-like receptors (TLRs)-mediated inflammatory innate response by stimulating the tumor necrosis factor alpha-induced protein TNFAIP3 expression via reactive oxygen species (ROS) in LPS-tolerized macrophages. Involved in antimicrobial response of innate immune cells; ACOD1-mediated itaconic acid production contributes to the antimicrobial activity of macrophages. Plays a role in the embryo implantation.
Show more

Research Articles on IRG1

1. In conclusion, we found that IRG1 promoted MHC I level in macrophages membrane via ROS-STAT1/3-TAP1 pathway. Besides, IRG1 could activate the pentose phosphate pathway to promote production of ROS depending on NADPH oxidases.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-IRG1 antibody

 Reference Product  PubMed Publications
 FLNA antibody  >12 publications with IRG1 and FLNA

Pathways associated with anti-IRG1 antibody

 Products by Pathway  Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000