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MAPK8IP1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS627163

JIP1 is a regulator of the pancreatic beta-cell function. It is highly similar to JIP-1, a mouse protein known to be a regulator of c-Jun amino-terminal kinase (Mapk8). This protein has been shown to prevent MAPK8 mediated activation of transcription factors,… More details

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Product Overview

Online Datasheet in PDF
Product Name
JIP1 (Mapk8ip1), Polyclonal Antibody
Full Product Name
JIP1, CT (C-Jun-amino-terminal Kinase-interacting Protein 1, JNK-interacting Protein 1, JIP-1, Islet-brain 1, IB1, IB-1, JNK MAP Kinase Scaffold Protein 1, Mitogen-activated Protein Kinase 8 Interacting Protein 1, MAPK8IP1, PRKM8IP)
Product Gene Name
anti-Mapk8ip1 antibody
Product Synonym Names
Anti -JIP1, CT (C-Jun-amino-terminal Kinase-interacting Protein 1, JNK-interacting Protein 1, JIP-1, Islet-brain 1, IB1, IB-1, JNK MAP Kinase Scaffold Protein 1, Mitogen-activated Protein Kinase 8 Interacting Protein 1, MAPK8IP1, PRKM8IP)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
Recognizes human JIP1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
77,318 Da
Chromosome Location
Chromosome: 3; NC_005102.2 (76781504..76790661, complement). Location: 3q24
3D Structure
ModBase 3D Structure for Q9R237
Immunogen
Synthetic peptide selected from the C-terminal region of human JIP1 (KLH).

Product Categories/Family for anti-Mapk8ip1 antibody

Antibodies; Abs to Protein Kinases
Applications Tested/Suitable for anti-Mapk8ip1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-Mapk8ip1 antibody
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500

Western Blot (WB) of anti-Mapk8ip1 antibody

Western blot analysis of JIP1 Antibody (C-term) in MCF-7 cell line lysates (35ug/lane). JIP1 (arrow) was detected using the purified Pab.
Rabbit Anti-MAPK8IP1 Polyclonal Antibody – Cat# MBS627163

Immunohistochemistry (IHC) of anti-Mapk8ip1 antibody

Formalin-fixed and paraffin-embedded human brain tissue reacted with JIP1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-MAPK8IP1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS627163

Flow Cytometry (FC/FACS) of anti-Mapk8ip1 antibody

JIP1 Antibody (C-term) flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-MAPK8IP1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS627163

Immunofluorescence (IF) of anti-Mapk8ip1 antibody

Confocal immunofluorescent analysis of JIP1 Antibody (C-term) with MCF-7 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-MAPK8IP1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS627163

Other Notes

Small volumes of anti-Mapk8ip1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Mapk8ip1 purchase

MBS627163 is an antibody targeting Mapk8ip1. Also known as JIP1, CT (C-Jun-amino-terminal Kinase-interacting Protein 1, JNK-interacting Protein 1, JIP-1, Islet-brain 1, IB1, IB-1, JNK MAP Kinase Scaffold Protein 1, Mitogen-activated Protein Kinase 8 Interacting Protein 1, MAPK8IP1, PRKM8IP). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Mapk8ip1. It may not necessarily be applicable to this product.

NCBI GI #
87130877
NCBI GeneID
116457
UniProt Primary Accession #
Q9R237
UniProt Secondary Accession #
O88979; Q9R1H8; Q9WVI5; Q9WVI6
UniProt Related Accession #
Q9R237
NCBI Official Full Name
JIP1 protein
NCBI Official Synonym Full Names
mitogen-activated protein kinase 8 interacting protein 1
NCBI Official Symbol
Mapk8ip1
NCBI Official Synonym Symbols
JIP1; Jip-1; Mapk8ip
UniProt Protein Name
C-Jun-amino-terminal kinase-interacting protein 1
UniProt Synonym Protein Names
Islet-brain-1; IB-1; JIP-1-related protein; JRP; JNK MAP kinase scaffold protein 1; Mitogen-activated protein kinase 8-interacting protein 1
Protein Family
C-Jun-amino-terminal kinase-interacting protein
UniProt Gene Name
Mapk8ip1
UniProt Synonym Gene Names
Ib1; Jip1; Mapk8ip
UniProt Entry Name
JIP1_RAT
NCBI Protein Information
C-Jun-amino-terminal kinase-interacting protein 1; JRP; IB-1; islet-brain-1; JIP-1-related protein; JNK-interacting protein 1; JNK MAP kinase scaffold protein 1; mitogen activated protein kinase 8 interacting protein; mitogen-activated protein kinase 8-interacting protein 1

NCBI Summary for Mapk8ip1

may play a role in the SAPK mediated stress-induced cellular responses [RGD]

UniProt Comments for Mapk8ip1

JIP1: a member of the JNK-interacting protein group of scaffold proteins that selectively mediates JNK signaling by aggregating specific components of the MAPK cascade. Required for JNK activation in response to excitotoxic stress. Causes inhibition of JNK-regulated activity by retaining JNK in the cytoplasm and by inhibiting the JNK phosphorylation of c-Jun. May also participate in ApoER2-specific reelin signaling. Directly or indirectly regulates GLUT2 gene expression and beta-cell function. Appears to have a role in cell signaling in mature and developing nerve terminals. May function as a regulator of vesicle transport, through interations with the JNK-signaling components and motor proteins.

Protein type: Adaptor/scaffold

Cellular Component: endoplasmic reticulum membrane; neuron projection; membrane; perinuclear region of cytoplasm; axon; mitochondrial membrane; cytoplasm; dendrite; synapse; nucleus; cytosol

Molecular Function: JUN kinase binding; identical protein binding; MAP-kinase scaffold activity; protein binding; kinesin binding; mitogen-activated protein kinase kinase binding; mitogen-activated protein kinase kinase kinase binding; protein kinase binding

Biological Process: vesicle-mediated transport; negative regulation of JNK activity; negative regulation of JNK cascade; positive regulation of signal transduction; regulation of transcription, DNA-dependent; signal transduction; regulation of JNK cascade; negative regulation of apoptosis; JUN phosphorylation
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Research Articles on Mapk8ip1

1. SOCS3 binds to the carboxyl terminus of C-Jun N-terminal kinase-interacting protein-1 (JIP1), increases its serine phosphorylation, and increases its binding to kinesin.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-Mapk8ip1 antibody

 Products by Pathway  Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 Wnt Signaling Pathway NetPath antibodies  Wnt Signaling Pathway NetPath Diagram

Diseases associated with anti-Mapk8ip1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >15 publications with Mapk8ip1 and Neoplasms
 Mental Disorders Antibodies  >12 publications with Mapk8ip1 and Mental Disorders
 Genital Diseases, Male Antibodies  >4 publications with Mapk8ip1 and Genital Diseases, Male
 Prostatic Diseases Antibodies  >3 publications with Mapk8ip1 and Prostatic Diseases
 Genetic Diseases, Inborn Antibodies  >3 publications with Mapk8ip1 and Genetic Diseases, Inborn
 Prostatic Neoplasms Antibodies  >3 publications with Mapk8ip1 and Prostatic Neoplasms
 Diabetes Mellitus, Type 2 Antibodies  >3 publications with Mapk8ip1 and Diabetes Mellitus, Type 2
 Congenital Abnormalities Antibodies  >2 publications with Mapk8ip1 and Congenital Abnormalities
 Melanoma Antibodies  >2 publications with Mapk8ip1 and Melanoma
 Obesity Antibodies  >1 publications with Mapk8ip1 and Obesity

Organs/Tissues associated with anti-Mapk8ip1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >27 publications with Mapk8ip1 and Brain
 Pancreas Antibodies  >9 publications with Mapk8ip1 and Pancreas
 Prostate Antibodies  >3 publications with Mapk8ip1 and Prostate
 Eye Antibodies  >2 publications with Mapk8ip1 and Eye
 Kidney Antibodies  >1 publications with Mapk8ip1 and Kidney
 Ovary Antibodies  >1 publications with Mapk8ip1 and Ovary
 Heart Antibodies  >1 publications with Mapk8ip1 and Heart
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