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KLF4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6313995

KLF4 is a transcription factor which acts as both an activator and repressor. Binds the CACCC core sequence. Binds to multiple sites in the 5'-flanking region of its own gene and can activate its own transcription. Required for establishing the barrier… More details

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Product Overview

Online Datasheet in PDF
Product Name
KLF4, Polyclonal Antibody
Full Product Name
KLF4, CT (KLF4, EZF, GKLF, Krueppel-like factor 4, Epithelial zinc finger protein EZF, Gut-enriched krueppel-like factor) (PE)
Product Gene Name
anti-KLF4 antibody
Product Synonym Names
KLF4; EZF; GKLF; Krueppel-like factor 4; Epithelial zinc finger protein EZF; Gut-enriched krueppel-like factor
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
58.1 kDa (545aa)
OMIM
602253
3D Structure
ModBase 3D Structure for O43474
Immunogen
KLF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 357-386 amino acids from the C-terminal region of human KLF4.
Conjugate
PE
Note
Preservative Free

Product Categories/Family for anti-KLF4 antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger
Applications Tested/Suitable for anti-KLF4 antibody
Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)
Application Notes for anti-KLF4 antibody
FC/FACS: 1:10-50
ELISA: 1:1,000
WB: 1:50-100
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-KLF4 antibody

KLF4 Antibody (C-term) western blot analysis in A431,MCF-7 cell line lysates (35ug/lane).This demonstrates the KLF4 antibody detected the KLF4 protein (arrow).
Rabbit Anti-KLF4 Polyclonal Antibody – Cat# MBS6313995

Immunofluorescence (IF) of anti-KLF4 antibody

Fluorescent confocal image of SY5Y cells stained with AP11958b KLF4 (C-term) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with AP11958b KLF4 (C-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 uM, 25 min). KLF4 immunoreactivity is localized very specifically to the nuclei of the SY5Y cells.
Rabbit Anti-KLF4 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS6313995

Flow Cytometry (FC/FACS) of anti-KLF4 antibody

KLF4 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-KLF4 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6313995

Product References and Citations for anti-KLF4 antibody

Guan, H., et al. Blood 116(9):1469-1478(2010). Wong, C.W., et al. Stem Cells 28(9):1510-1517(2010). Ohnesorge, N., et al. J. Biol. Chem. 285(34):26199-26210(2010). Cekanova, M., et al. Eur. J. Pharmacol. 637 (1-3), 30-37 (2010): Yori, J.L., et al. J. Biol. Chem. 285(22):16854-16863(2010).

Other Notes

Small volumes of anti-KLF4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KLF4 purchase

MBS6313995 is an antibody targeting KLF4. Also known as KLF4, CT (KLF4, EZF, GKLF, Krueppel-like factor 4, Epithelial zinc finger protein EZF, Gut-enriched krueppel-like factor) (PE). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for KLF4. It may not necessarily be applicable to this product.

NCBI GI #
194248077
NCBI GeneID
9314
NCBI Accession #
NP_004226
UniProt Primary Accession #
O43474
UniProt Secondary Accession #
P78338; Q5T3J8; Q5T3J9; Q8N717; Q9UNP3; B2R8S4; B3KT79; L0R3I6; L0R4N5
UniProt Related Accession #
O43474
GenBank Nucleotide #
NM_004235.5
NCBI Official Full Name
Krueppel-like factor 4 isoform 2
NCBI Official Synonym Full Names
Kruppel like factor 4
NCBI Official Symbol
KLF4
NCBI Official Synonym Symbols
EZF; GKLF
UniProt Protein Name
Krueppel-like factor 4
UniProt Synonym Protein Names
Epithelial zinc finger protein EZF; Gut-enriched krueppel-like factor
Protein Family
Krueppel-like factor
UniProt Gene Name
KLF4
UniProt Synonym Gene Names
EZF; GKLF
UniProt Entry Name
KLF4_HUMAN
NCBI Protein Information
Krueppel-like factor 4

NCBI Summary for KLF4

This gene encodes a protein that belongs to the Kruppel family of transcription factors. The encoded zinc finger protein is required for normal development of the barrier function of skin. The encoded protein is thought to control the G1-to-S transition of the cell cycle following DNA damage by mediating the tumor suppressor gene p53. Mice lacking this gene have a normal appearance but lose weight rapidly, and die shortly after birth due to fluid evaporation resulting from compromised epidermal barrier function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]
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Research Articles on KLF4

1. Data suggest that KLF4 could promote cell senescence through a complex network: miR-203, survivin, and p21, which were all regulated by overexpression of KLF4 and contributed to cell senescence.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-KLF4 antibody

 Reference Product  PubMed Publications
 SP1 antibody  >34 publications with KLF4 and SP1
 CTNNB1 antibody  >27 publications with KLF4 and CTNNB1
 TGFB1 antibody  >12 publications with KLF4 and TGFB1
 TP53 antibody  >11 publications with KLF4 and TP53
 HDAC2 antibody  >9 publications with KLF4 and HDAC2
 RELA antibody  >3 publications with KLF4 and RELA
 EP300 antibody  >2 publications with KLF4 and EP300
 KAT5 antibody  >1 publications with KLF4 and KAT5
 CREBBP antibody  >1 publications with KLF4 and CREBBP

Pathways associated with anti-KLF4 antibody

 Products by Pathway  Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Mesodermal Commitment Pathway antibodies  Mesodermal Commitment Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Peptide Hormone Metabolism Pathway antibodies  Peptide Hormone Metabolism Pathway Diagram
 Preimplantation Embryo Pathway antibodies  Preimplantation Embryo Pathway Diagram
 Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway antibodies  Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway Diagram
 SRF And MiRs In Smooth Muscle Differentiation And Proliferation Pathway antibodies  SRF And MiRs In Smooth Muscle Differentiation And Proliferation Pathway Diagram
 Signaling Pathways Regulating Pluripotency Of Stem Cells antibodies  Signaling Pathways Regulating Pluripotency Of Stem Cells Diagram
 Signaling Pathways Regulating Pluripotency Of Stem Cells antibodies  Signaling Pathways Regulating Pluripotency Of Stem Cells Diagram
 Synthesis, Secretion, And Deacylation Of Ghrelin Pathway antibodies  Synthesis, Secretion, And Deacylation Of Ghrelin Pathway Diagram

Diseases associated with anti-KLF4 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >244 publications with KLF4 and Neoplasms
 Cardiovascular Diseases Antibodies  >49 publications with KLF4 and Cardiovascular Diseases
 Nervous System Diseases Antibodies  >44 publications with KLF4 and Nervous System Diseases
 Inflammation Antibodies  >41 publications with KLF4 and Inflammation
 Disease Models, Animal Antibodies  >37 publications with KLF4 and Disease Models, Animal
 Breast Neoplasms Antibodies  >29 publications with KLF4 and Breast Neoplasms
 Brain Diseases Antibodies  >28 publications with KLF4 and Brain Diseases
 Adenocarcinoma Antibodies  >23 publications with KLF4 and Adenocarcinoma
 Heart Diseases Antibodies  >23 publications with KLF4 and Heart Diseases
 Carcinoma, Squamous Cell Antibodies  >16 publications with KLF4 and Carcinoma, Squamous Cell

Organs/Tissues associated with anti-KLF4 antibody

 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >153 publications with KLF4 and Embryonic Tissue
 Skin Antibodies  >103 publications with KLF4 and Skin
 Vascular Antibodies  >82 publications with KLF4 and Vascular
 Heart Antibodies  >57 publications with KLF4 and Heart
 Brain Antibodies  >49 publications with KLF4 and Brain
 Liver Antibodies  >43 publications with KLF4 and Liver
 Intestine Antibodies  >40 publications with KLF4 and Intestine
 Eye Antibodies  >35 publications with KLF4 and Eye
 Lung Antibodies  >28 publications with KLF4 and Lung
 Mouth Antibodies  >21 publications with KLF4 and Mouth
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