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KRT1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6314535

KRT1 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II… More details

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Product Overview

Online Datasheet in PDF
Product Name
KRT1, Polyclonal Antibody
Full Product Name
KRT1, ID (KRT1, KRTA, Keratin, type II cytoskeletal 1, 67kD cytokeratin, Cytokeratin-1, Hair alpha protein, Keratin-1, Type-II keratin Kb1) (AP)
Product Gene Name
Product Synonym Names
KRT1; KRTA; Keratin; type II cytoskeletal 1; 67kD cytokeratin; Cytokeratin-1; Hair alpha protein; Keratin-1; Type-II keratin Kb1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Purity/Purification
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Specificity
Recognizes human KRT1
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
66039 MW
OMIM
M98776 Genomic DNA
3D Structure
ModBase 3D Structure for P04264
Immunogen
KRT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 422~451 amino acids from the Central region of human KRT1.
Conjugate
AP
Note
Preservative Free

Product Categories/Family for anti-KRT1 antibody

Applications Tested/Suitable for anti-KRT1 antibody
Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-KRT1 antibody
FC/FACS: 1:10-50
ELISA: 1:1,000
IHC: 1:50-100
WB: 1:50-100
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-KRT1 antibody

Western blot analysis of KRT1 Antibody (Center) in MCF-7 cell line lysates (35ug/lane). KRT1 (arrow) was detected using the purified Pab.
Rabbit Anti-KRT1 Polyclonal Antibody – Cat# MBS6314535

Immunohistochemistry (IHC) of anti-KRT1 antibody

KRT1 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the KRT1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-KRT1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6314535

Flow Cytometry (FC/FACS) of anti-KRT1 antibody

KRT1 Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-KRT1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6314535

Product References and Citations for anti-KRT1 antibody

Labudova, M., et al. J. Virol. 83(16):7842-7849(2009). Barcelos, A.C., et al. J. Cutan. Pathol. 36(6):647-654(2009). Grimberg, G., et al. Br. J. Dermatol. 160(2):446-449(2009).

Other Notes

Small volumes of anti-KRT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KRT1 purchase

MBS6314535 is an antibody targeting KRT1. Also known as KRT1, ID (KRT1, KRTA, Keratin, type II cytoskeletal 1, 67kD cytokeratin, Cytokeratin-1, Hair alpha protein, Keratin-1, Type-II keratin Kb1) (AP). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for KRT1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
keratin, type II cytoskeletal 1
NCBI Official Synonym Full Names
keratin 1
NCBI Official Symbol
KRT1
NCBI Official Synonym Symbols
K1; CK1; EHK; EHK1; EPPK; KRT1A; NEPPK
UniProt Protein Name
Keratin, type II cytoskeletal 1
UniProt Synonym Protein Names
67 kDa cytokeratin; Cytokeratin-1; CK-1; Hair alpha protein; Keratin-1; K1; Type-II keratin Kb1
Protein Family
UniProt Gene Name
KRT1
UniProt Synonym Gene Names
KRTA; CK-1; K1
NCBI Protein Information
keratin, type II cytoskeletal 1

NCBI Summary for KRT1

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]
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UniProt Comments for KRT1

May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-KRT1 antibody

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