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KRT7 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS7048548

Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). More details

$230 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
KRT7, Polyclonal Antibody
Full Product Name
KRT7 Antibody
Product Gene Name
Product Synonym Names
Keratin; type II cytoskeletal 7; Cytokeratin-7; CK-7; Keratin-7; K7; Sarcolectin; Type-II keratin Kb7; KRT7; SCL
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid
Purity/Purification
>95%, Protein G purified
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
Matching Pairs
Unconjugated Antibody: KRT7 (MBS7048548)
HRP Conjugated Antibody: KRT7 (MBS7048549)
Matching Pairs
Unconjugated Antibody: KRT7 (MBS7048548)
FITC Conjugated Antibody: KRT7 (MBS7048550)
Molecular Weight
51,386 Da
OMIM
X03212 mRNA
3D Structure
ModBase 3D Structure for P08729
Immunogen
Recombinant Human Keratin, type II cytoskeletal 7 protein (2-469AA)
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, pH 7.4
Conjugation
Non-conjugated
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-17116 / sc-17118 / sc-25721
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-KRT7 antibody
ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-KRT7 antibody
IHC: 1: 20-1: 200
IF: 1: 50-1: 200

Immunohistochemistry (IHC) of anti-KRT7 antibody

Immunohistochemistry of paraffin-embedded human liver tissue using MBS7048548 at dilution of 1:100
Rabbit Anti-KRT7 Polyclonal Antibody – Cat# MBS7048548

Immunohistochemistry (IHC) of anti-KRT7 antibody

Immunohistochemistry of paraffin-embedded human cervical cancer using MBS7048548 at dilution of 1:100
Rabbit Anti-KRT7 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS7048548

Immunohistochemistry (IHC) of anti-KRT7 antibody

Immunofluorescent analysis of HepG2 cells using MBS7048548 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Rabbit Anti-KRT7 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS7048548

Western Blot (WB) of anti-KRT7 antibody

Western Blot
Positive WB detected in: Hela whole cell lysate,HepG2 whole cell lysate,A549 whole cell lysate
All lanes: KRT7 antibody at 3ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 52 KDa
Observed band size: 52 KDa
Rabbit Anti-KRT7 Polyclonal Antibody, Western Blot (WB) – Cat# MBS7048548

Immunoprecipitation (IP) of anti-KRT7 antibody

Immunoprecipitating KRT7 in HepG2 whole cell lysate
Lane 1: Rabbit monoclonal IgG (1ug)instead of MBS7048548 in HepG2 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: MBS7048548 (8ug)+ HepG2 whole cell lysate (500ug)
Lane 3: HepG2 whole cell lysate (10ug)
Rabbit Anti-KRT7 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS7048548

Other Notes

Small volumes of anti-KRT7 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KRT7 purchase

MBS7048548 is an antibody targeting KRT7. Also known as KRT7 Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for KRT7. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
keratin, type II cytoskeletal 7
NCBI Official Synonym Full Names
keratin 7
NCBI Official Symbol
KRT7
NCBI Official Synonym Symbols
K7; CK7; SCL; K2C7
UniProt Protein Name
Keratin, type II cytoskeletal 7
UniProt Synonym Protein Names
Cytokeratin-7; CK-7; Keratin-7; K7; Sarcolectin; Type-II keratin Kb7
Protein Family
UniProt Gene Name
KRT7
UniProt Synonym Gene Names
SCL; CK-7; K7
UniProt Entry Name
K2C7_HUMAN
NCBI Protein Information
keratin, type II cytoskeletal 7

NCBI Summary for KRT7

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]
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UniProt Comments for KRT7

K7: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Phosphorylation of keratins at specific sites affects their organization, assembly dynamics, and their interaction with signaling molecules. Specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-KRT7 antibody

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