Rabbit Anti-LAT Polyclonal Antibody – Cat# MBS630387

Western Blot analysis of SDS extracts from untreated or anti-CD3 treated (10 ug/ml for 2 minutes) human Jurkat cells after overnight serum starvation, and untreated or anti-CD3 treated (20 ug/ml for 2 minutes) mouse thymocyte, using Phospho-LAT (Tyr191) Antibody.

LAT Antibody – Rabbit Polyclonal

Name: LAT Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS630387
Price: 830 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS630387

LAT, a transmembrane adaptor protein expressed in T, NK and mast cells, is an important mediator for T cell receptor (TCR) signaling (1). Upon TCR engagement, activated ZAP70 phosphorylates LAT at multiple conserved tyrosine residues within SH2 binding… More details

$830 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

LAT, Polyclonal Antibody

Full Product Name

LAT, phosphorylated (Tyr191)

Product Gene Name

anti-LAT antibody

Product Synonym Names

Anti -LAT, phosphorylated (Tyr191)

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.

Purity/Purification

Affinity Purified
Purified by protein A and peptide affinity chromatography.

Specificity

Detects endogenous levels of LAT only when phosphorylated at tyrosine 191.

Preparation and Storage

May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

27,930 Da

Chromosome Location

Chromosome: 16; NC_000016.9 (28996147..29002104). Location: 16p11.2

OMIM

602354

3D Structure

ModBase 3D Structure for O43561

Immunogen

Synthetic phosphopeptide (KLH coupled) corresponding to residues around Tyr191 of human LAT.

Product Categories/Family for anti-LAT antibody

Antibodies; Abs to Proteins

Applications Tested/Suitable for anti-LAT antibody

Western Blot (WB), Immunoprecipitation (IP)

Application Notes for anti-LAT antibody

Suitable for use in Western Blotting, Immunoprecipitation.
Dilution: Western Blot: 1:1000
Immunoprecipitation: 1:100

Other Notes

Small volumes of anti-LAT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LAT purchase

MBS630387 is an antibody targeting LAT. Also known as LAT, phosphorylated (Tyr191). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for LAT. It may not necessarily be applicable to this product.

NCBI GI #

2828026

NCBI GeneID

27040

UniProt Primary Accession #

O43561

UniProt Secondary Accession #

O43919

UniProt Related Accession #

O43561

NCBI Official Full Name

LAT

NCBI Official Synonym Full Names

linker for activation of T cells

NCBI Official Symbol

LAT

NCBI Official Synonym Symbols

LAT1; pp36

UniProt Protein Name

Linker for activation of T-cells family member 1

UniProt Synonym Protein Names

36 kDa phospho-tyrosine adapter protein; pp36; p36-38

Protein Family

L-lysine-epsilon aminotransferase

UniProt Gene Name

LAT

UniProt Entry Name

LAT_HUMAN

NCBI Protein Information

linker for activation of T-cells family member 1; p36-38; OTTHUMP00000162563; OTTHUMP00000162564; 36 kDa phospho-tyrosine adapter protein; 36 kDa phospho-tyrosine adaptor protein

NCBI Summary for LAT

The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

UniProt Comments for LAT

LAT: an adaptor protein involved in T-cell antigen receptor (TCR) signal transduction pathway. Plays an important role downstream of protein tyrosine kinases. Essential for TCR-mediated Ras-Erk activation and calcium flux.

Protein type: Adaptor/scaffold; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: signalosome; mast cell granule; plasma membrane; integral to membrane; immunological synapse; intercellular junction; lipid raft

Molecular Function: protein binding; SH3/SH2 adaptor activity; protein kinase binding

Biological Process: integrin-mediated signaling pathway; platelet activation; positive regulation of signal transduction; T cell activation; calcium-mediated signaling; mast cell degranulation; T cell receptor signaling pathway; Ras protein signal transduction; innate immune response; gene expression; immune response; inflammatory response; blood coagulation; lymphocyte homeostasis; regulation of T cell activation

Research Articles on LAT

1. PECAM-1-mediated inhibition of GPVI-dependent platelet responses result from recruitment of SHP-2-p85 complexes to tyrosine-phosphorylated PECAM-1, which diminishes the association of PI3K with activatory signaling molecules Gab1 and LAT

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-LAT antibody

Reference Product	PubMed Publications
LCK antibody	>122 publications with LAT and LCK
SYK antibody	>116 publications with LAT and SYK
ZAP70 antibody	>108 publications with LAT and ZAP70
CD4 antibody	>104 publications with LAT and CD4
GRB2 antibody	>90 publications with LAT and GRB2
CBL antibody	>37 publications with LAT and CBL
VAV1 antibody	>34 publications with LAT and VAV1
ITK antibody	>24 publications with LAT and ITK
GRAP2 antibody	>24 publications with LAT and GRAP2
GAB2 antibody	>14 publications with LAT and GAB2

Pathways associated with anti-LAT antibody

Products by Pathway	Pathway Diagram
Adaptive Immunity Signaling Pathway antibodies	Adaptive Immunity Signaling Pathway Diagram
Fc Epsilon RI Signaling Pathway antibodies	Fc Epsilon RI Signaling Pathway Diagram
Fc Epsilon RI Signaling Pathway antibodies	Fc Epsilon RI Signaling Pathway Diagram
Fc Gamma R-mediated Phagocytosis Pathway antibodies	Fc Gamma R-mediated Phagocytosis Pathway Diagram
Fc Gamma R-mediated Phagocytosis Pathway antibodies	Fc Gamma R-mediated Phagocytosis Pathway Diagram
Fc-epsilon Receptor I Signaling In Mast Cells Pathway antibodies	Fc-epsilon Receptor I Signaling In Mast Cells Pathway Diagram
Formation Of Platelet Plug Pathway antibodies	Formation Of Platelet Plug Pathway Diagram
GPVI-mediated Activation Cascade Pathway antibodies	GPVI-mediated Activation Cascade Pathway Diagram
Generation Of Second Messenger Molecules Pathway antibodies	Generation Of Second Messenger Molecules Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram

Diseases associated with anti-LAT antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>115 publications with LAT and Neoplasms
Nervous System Diseases Antibodies	>114 publications with LAT and Nervous System Diseases
Death Antibodies	>52 publications with LAT and Death
Inflammation Antibodies	>45 publications with LAT and Inflammation
Respiratory Tract Diseases Antibodies	>37 publications with LAT and Respiratory Tract Diseases
Thrombosis Antibodies	>36 publications with LAT and Thrombosis
Leukemia Antibodies	>34 publications with LAT and Leukemia
Recurrence Antibodies	>32 publications with LAT and Recurrence
Pain Antibodies	>31 publications with LAT and Pain
Carcinoma Antibodies	>31 publications with LAT and Carcinoma

Organs/Tissues associated with anti-LAT antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>728 publications with LAT and Blood
Brain Antibodies	>167 publications with LAT and Brain
Muscle Antibodies	>144 publications with LAT and Muscle
Heart Antibodies	>136 publications with LAT and Heart
Bone Antibodies	>130 publications with LAT and Bone
Eye Antibodies	>107 publications with LAT and Eye
Nerve Antibodies	>67 publications with LAT and Nerve
Skin Antibodies	>67 publications with LAT and Skin
Kidney Antibodies	>49 publications with LAT and Kidney
Liver Antibodies	>48 publications with LAT and Liver