• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

LDLR Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1758026

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
LDLR, Polyclonal Antibody
Full Product Name
Anti-LDLR Antibody Picoband
Product Gene Name
anti-LDLR antibody
Product Synonym Names
low density lipoprotein receptor; Low-density lipoprotein receptor; LDL receptor; LDLR
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Specificity
Widely expressed, including in adult and fetal brain, placenta, skin fibroblasts, adipose tissue and gonads.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
Molecular Weight
95,376 Da
Chromosome Location
Chromosome: 19; NC_000019.9 (11200038..11244506). Location: 19p13.2
OMIM
143890
3D Structure
ModBase 3D Structure for P01130
Immunogen
E coli-derived human LDLR recombinant protein (Position: A705-A860).
Subcellular Localization
Lysosome. Golgi apparatus. Cell membrane. Single-pass type I membrane protein. Early endosome. Late endosome. Clathrin-coated pit.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross-reactivity with other proteins.
Protein Function
Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. (Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes, but not through a interaction with viral proteins. (Microbial infection) Acts as a receptor for Vesicular stomatitis virus. (Microbial infection) In case of HIV-1 infection, may function as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells.

Product Categories/Family for anti-LDLR antibody

Primary Antibodies; Cancer; Cardiovascular; Cytochromes; Drug Metabolism; Growth Factors/Hormones; Lipases; Lipid and Lipoprotein Metabolism; Lipid Metabolism; Lipids/Lipoproteins; Metabolic Signaling Pathways; Metabolism; Mitochondrial Metabolism; Pathwa
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-LDLR antibody
Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-LDLR antibody
WB: 0.25-0.5ug/ml, Human
FC/FACS (Fixed): 1-3ug/1x1x106 cells, Human
ELISA: 0.1-0.5ug/ml

Western Blot (WB) of anti-LDLR antibody

Figure 1. Western blot analysis of LDLR using anti-LDLR antibody (MBS1758026).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LDLR antigen affinity purified polyclonal antibody (#MBS1758026) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for LDLR at approximately 140-160 kDa. The expected band size for LDLR is at 95,140-160,120 kDa.
Rabbit Anti-LDLR Polyclonal Antibody – Cat# MBS1758026

Flow Cytometry (FC/FACS) of anti-LDLR antibody

Figure 2. Flow Cytometry analysis of MCF-7 cells using anti-LDLR antibody (MBS1758026).
Overlay histogram showing MCF-7 cells stained with MBS1758026 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LDLR Antibody (MBS1758026, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (MBS177405, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Rabbit Anti-LDLR Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1758026

Product References and Citations for anti-LDLR antibody

1. Aalto-Setala, K., Helve, E., Kovanen, P. T., Kontula, K. Finnish type of low absorbance lipoprotein receptor gene mutation (FH-Helsinki) deletes exons encoding the carboxy-terminal part of the receptor and creates an internalization-defective phenotype. J. Clin. Invest. 84: 499-505, 1989.2. Aalto-Setala, K., Koivisto, U.-M., Miettinen, T. A., Gylling, H., Kesaniemi, Y. A., Savolainen, M., Pyorala, K., Ebeling, T., Mononen, I., Turtola, H., Viikari, J., Kontula, K. Prevalence and geographical distribution of major LDL receptor gene rearrangements in Finland. J. Intern. Med. 231: 227-234, 1992.3. Agnello, V., Abel, G., Elfahal, M., Knight, G. B., Zhang, Q.-X. Hepatitis C virus and other flaviviridae viruses enter cells via low absorbance lipoprotein receptor. Proc. Nat. Acad. Sci. 96: 12766-12771, 1999.

Other Notes

Small volumes of anti-LDLR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LDLR purchase

MBS1758026 is an antibody targeting LDLR. Also known as Anti-LDLR Antibody Picoband. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for LDLR. It may not necessarily be applicable to this product.

NCBI GI #
4504975
NCBI GeneID
3949
NCBI Accession #
NP_000518.1
UniProt Primary Accession #
P01130
UniProt Secondary Accession #
Q53ZD9; Q59FQ1; Q9UDH7; B4DII3; B4DJZ8; B4DR00; B4DTQ3; C0JYY8; H0YLU8; H0YNT7
UniProt Related Accession #
P01130
GenBank Nucleotide #
NM_000527.4
NCBI Official Full Name
low-density lipoprotein receptor isoform 1
NCBI Official Synonym Full Names
low density lipoprotein receptor
NCBI Official Symbol
LDLR
NCBI Official Synonym Symbols
FH; FHC; LDLCQ2
UniProt Protein Name
Low-density lipoprotein receptor
Protein Family
Low-density lipoprotein receptor
UniProt Gene Name
LDLR
UniProt Synonym Gene Names
LDL receptor
UniProt Entry Name
LDLR_HUMAN
NCBI Protein Information
low-density lipoprotein receptor; LDL receptor; low-density lipoprotein receptor class A domain-containing protein 3

NCBI Summary for LDLR

The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2010]
Show more

UniProt Comments for LDLR

LDLR: Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells. Defects in LDLR are the cause of familial hypercholesterolemia (FH); a common autosomal semi- dominant disease that affects about 1 in 500 individuals. The receptor defect impairs the catabolism of LDL, and the resultant elevation in plasma LDL-cholesterol promotes deposition of cholesterol in the skin (xanthelasma), tendons (xanthomas), and coronary arteries (atherosclerosis). Belongs to the LDLR family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: Golgi apparatus; cell surface; membrane; integral to plasma membrane; lysosome; late endosome; early endosome; plasma membrane; endosome membrane; coated pit; receptor complex; external side of plasma membrane

Molecular Function: low-density lipoprotein receptor activity; very-low-density lipoprotein receptor activity; protein binding; low-density lipoprotein binding; clathrin heavy chain binding; calcium ion binding; glycoprotein binding

Biological Process: lipoprotein catabolic process; cholesterol metabolic process; phototransduction, visible light; receptor-mediated endocytosis; cholesterol transport; viral reproduction; cholesterol absorption; endocytosis; lipoprotein metabolic process; cholesterol homeostasis; phospholipid transport; lipid metabolic process; retinoid metabolic process

Disease: Hypercholesterolemia, Familial
Show more

Research Articles on LDLR

1. The LDL receptor (LDLR) serves as the major entry port of vesicular stomatitis virus and of VSV-G oat protein pseudotyped lentiviral vectors in human and mouse cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-LDLR antibody

 Reference Product  PubMed Publications
 APOE antibody  >474 publications with LDLR and APOE
 APOB antibody  >368 publications with LDLR and APOB
 PCSK9 antibody  >248 publications with LDLR and PCSK9
 LDLRAP1 antibody  >25 publications with LDLR and LDLRAP1
 LIPC antibody  >14 publications with LDLR and LIPC
 MYLIP antibody  >10 publications with LDLR and MYLIP
 DAB1 antibody  >3 publications with LDLR and DAB1
 LRPAP1 antibody  >3 publications with LDLR and LRPAP1
 APOH antibody  >1 publications with LDLR and APOH

Pathways associated with anti-LDLR antibody

 Products by Pathway  Pathway Diagram
 Bile Secretion Pathway antibodies  Bile Secretion Pathway Diagram
 Bile Secretion Pathway antibodies  Bile Secretion Pathway Diagram
 Chylomicron-mediated Lipid Transport Pathway antibodies  Chylomicron-mediated Lipid Transport Pathway Diagram
 DNA Damage Response (only ATM Dependent) Pathway antibodies  DNA Damage Response (only ATM Dependent) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Diseases Associated With Visual Transduction Pathway antibodies  Diseases Associated With Visual Transduction Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Hepatitis C Pathway antibodies  Hepatitis C Pathway Diagram
 Hepatitis C Pathway antibodies  Hepatitis C Pathway Diagram

Diseases associated with anti-LDLR antibody

 Disease Name  Pubmed Publications
 Atherosclerosis Antibodies  >952 publications with LDLR and Atherosclerosis
 Cardiovascular Diseases Antibodies  >842 publications with LDLR and Cardiovascular Diseases
 Hypercholesterolemia Antibodies  >743 publications with LDLR and Hypercholesterolemia
 Dyslipidemias Antibodies  >737 publications with LDLR and Dyslipidemias
 Hyperlipidemias Antibodies  >689 publications with LDLR and Hyperlipidemias
 HYPERCHOLESTEROLEMIA, FAMILIAL Antibodies  >491 publications with LDLR and HYPERCHOLESTEROLEMIA, FAMILIAL
 Hyperlipoproteinemia Type II Antibodies  >355 publications with LDLR and Hyperlipoproteinemia Type II
 Inflammation Antibodies  >233 publications with LDLR and Inflammation
 Coronary Artery Disease Antibodies  >227 publications with LDLR and Coronary Artery Disease
 Coronary Disease Antibodies  >226 publications with LDLR and Coronary Disease

Organs/Tissues associated with anti-LDLR antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1058 publications with LDLR and Blood
 Liver Antibodies  >588 publications with LDLR and Liver
 Vascular Antibodies  >513 publications with LDLR and Vascular
 Heart Antibodies  >275 publications with LDLR and Heart
 Bone Antibodies  >189 publications with LDLR and Bone
 Muscle Antibodies  >171 publications with LDLR and Muscle
 Bone Marrow Antibodies  >159 publications with LDLR and Bone Marrow
 Brain Antibodies  >125 publications with LDLR and Brain
 Kidney Antibodies  >69 publications with LDLR and Kidney
 Ovary Antibodies  >58 publications with LDLR and Ovary
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000