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LGR5 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6315928

LGR5/GPR49 is an orphan receptor. It may be an important receptor for signals controlling growth and differentiation of specific embryonic tissues. More details

$1060 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
LGR5, Polyclonal Antibody
Full Product Name
LGR5, NT (LGR5, GPR49, GPR67, Leucine-rich repeat-containing G-protein coupled receptor 5, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38) (MaxLight 550)
Product Gene Name
anti-LGR5 antibody
Product Synonym Names
LGR5; GPR49; GPR67; Leucine-rich repeat-containing G-protein coupled receptor 5; G-protein coupled receptor 49; G-protein coupled receptor 67; G-protein coupled receptor HG38
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.
Purity/Purification
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
99998 MW
OMIM
AF062006 mRNA
3D Structure
ModBase 3D Structure for O75473
Immunogen
LGR5/GPR49 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 247~277 amino acids from the N-terminal region of human LGR5.
Conjugate
MaxLight550
Note
Preservative Free

Product Categories/Family for anti-LGR5 antibody

Antibodies; G Protein Receptors
Applications Tested/Suitable for anti-LGR5 antibody
Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-LGR5 antibody
FC/FACS: 1:10-50
FLISA: 1:1,000
IF: 1:10-50
IHC: 1:10-50
WB: 1:50-100
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-LGR5 antibody

LGR5/GPR49 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow)(Kindly offered by Dr. Li).
Rabbit Anti-LGR5 Polyclonal Antibody – Cat# MBS6315928

Immunohistochemistry (IHC) of anti-LGR5 antibody

Formalin-fixed and paraffin-embedded human brain tissue reacted with LGR5/GPR49 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-LGR5 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6315928

Flow Cytometry (FC/FACS) of anti-LGR5 antibody

Flow cytometric analysis of HepG2 cells using LGR5/GPR49 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-LGR5 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6315928

Immunofluorescence (IF) of anti-LGR5 antibody

Confocal immunofluorescent analysis of LGR5/GPR49 Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-LGR5 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS6315928

Product References and Citations for anti-LGR5 antibody

Barker, N., Nature 449 (7165), 1003-1007 (2007). McClanahan, T., Cancer Biol. Ther. 5 (4), 419-426 (2006). Yamamoto, Y., Hepatology 37 (3), 528-533 (2003). Hsu, S.Y., Mol. Endocrinol. 14 (8), 1257-1271 (2000).

Other Notes

Small volumes of anti-LGR5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LGR5 purchase

MBS6315928 is an antibody targeting LGR5. Also known as LGR5, NT (LGR5, GPR49, GPR67, Leucine-rich repeat-containing G-protein coupled receptor 5, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38) (MaxLight 550). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for LGR5. It may not necessarily be applicable to this product.

NCBI GI #
469608414
NCBI GeneID
8549
NCBI Accession #
NP_001264155.1
UniProt Primary Accession #
O75473
UniProt Secondary Accession #
Q4VAM0; Q4VAM2; Q9UP75; D8MCT0
UniProt Related Accession #
O75473
GenBank Nucleotide #
NM_001277226.1
NCBI Official Full Name
leucine-rich repeat-containing G-protein coupled receptor 5 isoform 2
NCBI Official Synonym Full Names
leucine rich repeat containing G protein-coupled receptor 5
NCBI Official Symbol
LGR5
NCBI Official Synonym Symbols
FEX; HG38; GPR49; GPR67; GRP49
UniProt Protein Name
Leucine-rich repeat-containing G-protein coupled receptor 5
UniProt Synonym Protein Names
G-protein coupled receptor 49; G-protein coupled receptor 67; G-protein coupled receptor HG38
Protein Family
Leucine-rich repeat-containing G-protein coupled receptor
UniProt Gene Name
LGR5
UniProt Synonym Gene Names
GPR49; GPR67
NCBI Protein Information
leucine-rich repeat-containing G-protein coupled receptor 5

NCBI Summary for LGR5

The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
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UniProt Comments for LGR5

Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.
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Research Articles on LGR5

1. This study uncovers a novel cross-talk between LGR5 and TGFbeta signaling in colon cancer and identifies LGR5 as a new modulator of TGFbeta signaling able to suppress colon cancer metastasis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-LGR5 antibody

 Reference Product  PubMed Publications
 OLFM4 antibody  >18 publications with LGR5 and OLFM4
 DCLK1 antibody  >16 publications with LGR5 and DCLK1
 TSPAN8 antibody  >13 publications with LGR5 and TSPAN8
 JAZF1 antibody  >13 publications with LGR5 and JAZF1
 ADAMTS9 antibody  >11 publications with LGR5 and ADAMTS9
 CAMK1D antibody  >10 publications with LGR5 and CAMK1D
 THADA antibody  >10 publications with LGR5 and THADA
 CDC123 antibody  >10 publications with LGR5 and CDC123
 MBD3 antibody  >1 publications with LGR5 and MBD3

Pathways associated with anti-LGR5 antibody

 Products by Pathway  Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 RNF Mutants Show Enhanced WNT Signaling And Proliferation Pathway antibodies  RNF Mutants Show Enhanced WNT Signaling And Proliferation Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By WNT In Cancer Pathway antibodies  Signaling By WNT In Cancer Pathway Diagram
 Signaling By Wnt Pathway antibodies  Signaling By Wnt Pathway Diagram
 TCF Dependent Signaling In Response To WNT Pathway antibodies  TCF Dependent Signaling In Response To WNT Pathway Diagram
 XAV939 Inhibits Tankyrase, Stabilizing AXIN Pathway antibodies  XAV939 Inhibits Tankyrase, Stabilizing AXIN Pathway Diagram
 Misspliced LRP5 Mutants Have Enhanced Beta-catenin-dependent Signaling Pathway antibodies  Misspliced LRP5 Mutants Have Enhanced Beta-catenin-dependent Signaling Pathway Diagram
 Regulation Of FZD By Ubiquitination Pathway antibodies  Regulation Of FZD By Ubiquitination Pathway Diagram

Diseases associated with anti-LGR5 antibody

 Disease Name  Pubmed Publications
 Colonic Neoplasms Antibodies  >51 publications with LGR5 and Colonic Neoplasms
 Cell Transformation, Neoplastic Antibodies  >30 publications with LGR5 and Cell Transformation, Neoplastic
 Inflammation Antibodies  >25 publications with LGR5 and Inflammation
 Neoplasm Metastasis Antibodies  >24 publications with LGR5 and Neoplasm Metastasis
 Liver Diseases Antibodies  >18 publications with LGR5 and Liver Diseases
 Liver Neoplasms Antibodies  >18 publications with LGR5 and Liver Neoplasms
 Disease Models, Animal Antibodies  >15 publications with LGR5 and Disease Models, Animal
 Neoplasms, Experimental Antibodies  >13 publications with LGR5 and Neoplasms, Experimental
 Precancerous Conditions Antibodies  >10 publications with LGR5 and Precancerous Conditions
 Necrosis Antibodies  >8 publications with LGR5 and Necrosis

Organs/Tissues associated with anti-LGR5 antibody

 Organ/Tissue Name  Pubmed Publications
 Intestine Antibodies  >201 publications with LGR5 and Intestine
 Liver Antibodies  >45 publications with LGR5 and Liver
 Stomach Antibodies  >43 publications with LGR5 and Stomach
 Skin Antibodies  >28 publications with LGR5 and Skin
 Embryonic Tissue Antibodies  >25 publications with LGR5 and Embryonic Tissue
 Bone Antibodies  >20 publications with LGR5 and Bone
 Brain Antibodies  >11 publications with LGR5 and Brain
 Lymph Node Antibodies  >11 publications with LGR5 and Lymph Node
 Ovary Antibodies  >11 publications with LGR5 and Ovary
 Muscle Antibodies  >8 publications with LGR5 and Muscle
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