• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

LIGHT Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS695366

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hLIGHT. Anti-Human LIGHT specific antibody was purified by affinity chromatography employing immobilized hLIGHT matrix. More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Product Name
LIGHT, Polyclonal Antibody
Full Product Name
Anti-human LIGHT
Product Gene Name
anti-LIGHT antibody
Product Synonym Names
Anti-Human LIGHT
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Purified
Preparation and Storage
At -20 degree C
Molecular Weight
22,395 Da
OMIM
604520
3D Structure
ModBase 3D Structure for O43557
Immunogen
Recombinant Human LIGHT
Applications Tested/Suitable for anti-LIGHT antibody
Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-LIGHT antibody
Immunohistochemistery: This antibody stained formalin-fixed, paraffin-embedded sections of human normal placenta. The recommended concentration is 2.5 ug/mL with an overnight incubation at 4 degree C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 8.0 buffer is recommended. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Information and photo are courtesy of Flagship Biosciences, LLC - Histology Services.

Sandwich ELISA: To detect hLIGHT by sandwich ELISA (using 100 mul/well antibody solution) a concentration of 0.5 - 2.0 mug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human LIGHT as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hLIGHT.

Western Blot: To detect hLIGHT by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLIGHT is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Other Notes

Small volumes of anti-LIGHT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LIGHT purchase

MBS695366 is an antibody targeting LIGHT. Also known as Anti-human LIGHT. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for LIGHT. It may not necessarily be applicable to this product.

NCBI GI #
313104028
NCBI GeneID
8740
NCBI Accession #
O43557.2
UniProt Primary Accession #
O43557
UniProt Secondary Accession #
O75476; Q6FHA1; Q8WVF8; Q96LD2; A8K7M2; C9J5H4
UniProt Related Accession #
O43557
NCBI Official Full Name
Tumor necrosis factor ligand superfamily member 14
NCBI Official Synonym Full Names
tumor necrosis factor (ligand) superfamily, member 14
NCBI Official Symbol
TNFSF14
NCBI Official Synonym Symbols
LTg; TR2; CD258; HVEML; LIGHT
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 14
UniProt Synonym Protein Names
Herpes virus entry mediator ligand; HVEM-L; Herpesvirus entry mediator ligand; CD_antigen: CD258Cleaved into the following 2 chains:Tumor necrosis factor ligand superfamily member 14, membrane form; Tumor necrosis factor ligand superfamily member 14, soluble form
Protein Family
Light-activated DNA-binding protein
UniProt Gene Name
TNFSF14
UniProt Synonym Gene Names
HVEML; LIGHT; HVEM-L; Herpesvirus entry mediator ligand
UniProt Entry Name
TNF14_HUMAN
NCBI Protein Information
tumor necrosis factor ligand superfamily member 14; delta transmembrane LIGHT; herpesvirus entry mediator A; herpesvirus entry mediator ligand; herpesvirus entry mediator-ligand; herpes virus entry mediator ligand; ligand for herpesvirus entry mediator; tumor necrosis factor receptor-like 2; tumor necrosis factor superfamily member LIGHT

NCBI Summary for LIGHT

The protein encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF14, which is a member of the tumor necrosis factor receptor superfamily, and which is also known as a herpesvirus entry mediator (HVEM). This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells. This protein is also reported to prevent tumor necrosis factor alpha mediated apoptosis in primary hepatocyte. Two alternatively spliced transcript variant encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Show more

UniProt Comments for LIGHT

TNFSF14: Cytokine that binds to TNFRSF3/LTBR. Binding to the decoy receptor TNFRSF6B modulates its effects. Activates NFKB, stimulates the proliferation of T-cells, and inhibits growth of the adenocarcinoma HT-29. Acts as a receptor for Herpes simplex virus. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cytokine; Inhibitor

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: extracellular space; cytoplasm; plasma membrane; integral to membrane

Molecular Function: protein binding; caspase inhibitor activity; cytokine activity; tumor necrosis factor receptor binding; receptor binding

Biological Process: release of cytoplasmic sequestered NF-kappaB; T cell proliferation; T cell activation; apoptosis; T cell costimulation; T cell homeostasis; immune response; negative regulation of caspase activity; signal transduction; positive regulation of myoblast differentiation
Show more

Research Articles on LIGHT

1. Triggering of LIGHT induced production of pro-inflammatory mediators such as interleukin-8 and matrix metalloproteinase-9 while suppressing the phagocytic activity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-LIGHT antibody

 Reference Product  PubMed Publications
 TNFRSF14 antibody  >66 publications with LIGHT and TNFRSF14
 LTBR antibody  >39 publications with LIGHT and LTBR
 TNFRSF6B antibody  >14 publications with LIGHT and TNFRSF6B
 BTLA antibody  >13 publications with LIGHT and BTLA
 TRAF3 antibody  >5 publications with LIGHT and TRAF3
 DIABLO antibody  >2 publications with LIGHT and DIABLO

Pathways associated with anti-LIGHT antibody

 Products by Pathway  Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Herpes Simplex Infection Pathway antibodies  Herpes Simplex Infection Pathway Diagram
 Herpes Simplex Infection Pathway antibodies  Herpes Simplex Infection Pathway Diagram
 NF-kappa B Signaling Pathway antibodies  NF-kappa B Signaling Pathway Diagram

Diseases associated with anti-LIGHT antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >219 publications with LIGHT and Necrosis
 Inflammation Antibodies  >59 publications with LIGHT and Inflammation
 Immune System Diseases Antibodies  >43 publications with LIGHT and Immune System Diseases
 Disease Models, Animal Antibodies  >21 publications with LIGHT and Disease Models, Animal
 Liver Diseases Antibodies  >14 publications with LIGHT and Liver Diseases
 Neoplasms, Experimental Antibodies  >11 publications with LIGHT and Neoplasms, Experimental
 Cardiovascular Diseases Antibodies  >10 publications with LIGHT and Cardiovascular Diseases
 Brain Diseases Antibodies  >7 publications with LIGHT and Brain Diseases
 Lung Diseases Antibodies  >7 publications with LIGHT and Lung Diseases
 Breast Neoplasms Antibodies  >5 publications with LIGHT and Breast Neoplasms

Organs/Tissues associated with anti-LIGHT antibody

 Organ/Tissue Name  Pubmed Publications
 Spleen Antibodies  >22 publications with LIGHT and Spleen
 Liver Antibodies  >15 publications with LIGHT and Liver
 Brain Antibodies  >7 publications with LIGHT and Brain
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000