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LRP Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6485420

MBS6485420 is an antibody targeting LRP. Also known as LRP, CT (Major Vault Protein, Lung Resistance-related Protein, MVP) (Biotin). Reactive in Human. Host: Rabbit. More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
LRP, Polyclonal Antibody
Full Product Name
LRP, CT (Major Vault Protein, Lung Resistance-related Protein, MVP) (Biotin)
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Purity/Purification
Purified by Protein G Affinity Chromatography.
Specificity
Recognizes human MVP.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
99,327 Da
OMIM
605088
3D Structure
ModBase 3D Structure for Q14764
Immunogen
Synthetic peptide selected from the C-terminal region of human MVP (KLH).
Conjugate
Biotin
Note
Preservative Free
Applications Tested/Suitable for anti-LRP antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes for anti-LRP antibody
IHC: 1:50-1:100
ELISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-LRP antibody

The anti-MVP C-term Antibody is used in Western blot to detect MVP in A375 lysate.
Rabbit Anti-LRP Polyclonal Antibody – Cat# MBS6485420

Western Blot (WB) of anti-LRP antibody

Western blot analysis of MVP (arrow) using rabbit polyclonal MVP C-term Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MVP gene (Lane 2) (Origene Technologies).
Rabbit Anti-LRP Polyclonal Antibody, Western Blot (WB) – Cat# MBS6485420

Immunohistochemistry (IHC) of anti-LRP antibody

Formalin-fixed and paraffin-embedded human lung carcinoma with MVP Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-LRP Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6485420

Flow Cytometry (FC/FACS) of anti-LRP antibody

Flow cytometric analysis of NCI-H292 cells using MVP Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-LRP Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6485420

Product References and Citations for anti-LRP antibody

1. Aronica, E., et al., Epilepsia 44(9):1166-1175 (2003). 2. Klunder, J.W., et al., Hum. Pathol. 34(2):150-155 (2003). 3. Burger, H., et al., Clin. Cancer Res. 9(2):827-836 (2003). 4. Yu, Z., et al., J. Biol. Chem. 277(43):40247-40252 (2002). 5. van den Heuvel-Eibrink, M.M., et al., Leukemia 16(5):833-839 (2002).

Other Notes

Small volumes of anti-LRP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for LRP. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
major vault protein isoform 1
NCBI Official Synonym Full Names
major vault protein
NCBI Official Symbol
MVP
NCBI Official Synonym Symbols
LRP; VAULT1
UniProt Protein Name
Major vault protein
UniProt Synonym Protein Names
Lung resistance-related protein
UniProt Gene Name
MVP
UniProt Synonym Gene Names
LRP; MVP
UniProt Entry Name
MVP_HUMAN
NCBI Protein Information
major vault protein; lung resistance-related protein

UniProt Comments for LRP

MVP: Required for normal vault structure. Vaults are multi- subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo- cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: cytoskeleton; membrane; perinuclear region of cytoplasm; cytoplasm; nuclear pore; nucleus; ribonucleoprotein complex

Molecular Function: protein binding; protein phosphatase binding; protein kinase binding

Biological Process: cell proliferation; protein transport; mRNA transport; negative regulation of protein amino acid autophosphorylation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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