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LTA4H Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9209058

DHCR24 hydrolyzes an epoxide moiety of leukotriene A4 (LTA-4) to form leukotriene B4 (LTB-4). This enzyme also has some peptidase activity. More details

$240 / 0.08 mL +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
LTA4H, Polyclonal Antibody
Full Product Name
LTA4H Antibody (Center)
Product Gene Name
Product Synonym Names
Leukotriene A-4 hydrolase; LTA-4 hydrolase; Leukotriene A(4) hydrolase; LTA4H; LTA4
Clonality
Polyclonal
Isotype
Rabbit Ig
Host
Rabbit
Species Reactivity
Human
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Concentration
Vial Concentration: 0.5 (lot specific)
Specificity
This LTA4H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 163-191 amino acids from the Central region of human LTA4H.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
LTA4H peptide (MBS9224880) is used for blocking the activity of LTA4H antibody (MBS9209058)
Molecular Weight
69285
Immunogen Sequence Positions
163-191
OMIM
151570
3D Structure
ModBase 3D Structure for P09960
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Applications Tested/Suitable for anti-LTA4H antibody
Immunofluorescence (IF), ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-LTA4H antibody
WB~~1:1000

Western Blot (WB) of anti-LTA4H antibody

Western blot analysis of LTA4H Antibody (Center) in T47D cell line lysates (35ug/lane). LTA4H (arrow) was detected using the purified Pab.
Rabbit Anti-LTA4H Polyclonal Antibody – Cat# MBS9209058

Immunohistochemistry (IHC) of anti-LTA4H antibody

Formalin-fixed and paraffin-embedded human lymph tissue reacted with LTA4H Antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-LTA4H Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9209058

Immunofluorescence (IF) of anti-LTA4H antibody

Confocal immunofluorescent analysis of LTA4H Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-LTA4H Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS9209058

Product References and Citations for anti-LTA4H antibody

Bevan,S., Stroke 40 (3), 696-701 (2009) Crosslin,D.R., Hum. Genet. 125 (2), 217-229 (2009) Huston,A.L., Biochim. Biophys. Acta 1784 (11), 1865-1872 (2008) Rybina,I.V., J. Biol. Chem. 272 (50), 31865-31871 (1997)

Other Notes

Small volumes of anti-LTA4H antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LTA4H purchase

MBS9209058 is an antibody targeting LTA4H. Also known as LTA4H Antibody (Center). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for LTA4H. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
leukotriene A-4 hydrolase isoform 1
NCBI Official Synonym Full Names
leukotriene A4 hydrolase
NCBI Official Symbol
LTA4H
UniProt Protein Name
Leukotriene A-4 hydrolase
UniProt Synonym Protein Names
Leukotriene A(4) hydrolase
UniProt Gene Name
LTA4H
UniProt Synonym Gene Names
LTA4; LTA-4 hydrolase
UniProt Entry Name
LKHA4_HUMAN
NCBI Protein Information
leukotriene A-4 hydrolase

NCBI Summary for LTA4H

The protein encoded by this gene is an enzyme that contains both hydrolase and aminopeptidase activities. The hydrolase activity is used in the final step of the biosynthesis of leukotriene B4, a proinflammatory mediator. The aminopeptidase activity has been shown to degrade proline-glycine-proline (PGP), a neutrophil chemoattractant and biomarker for chronic obstructive pulmonary disease (COPD). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
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UniProt Comments for LTA4H

LTA4H: Epoxide hydrolase that catalyzes the final step in the biosynthesis of the proinflammatory mediator leukotriene B4. Has also aminopeptidase activity. Belongs to the peptidase M1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - arachidonic acid; EC 3.3.2.6; Hydrolase

Chromosomal Location of Human Ortholog: 12q22

Cellular Component: nucleoplasm; cytoplasm; cytosol

Molecular Function: peptidase activity; leukotriene-A4 hydrolase activity; zinc ion binding; metallopeptidase activity; epoxide hydrolase activity; aminopeptidase activity

Biological Process: leukotriene biosynthetic process; peptide catabolic process; arachidonic acid metabolic process; leukotriene metabolic process; inflammatory response; proteolysis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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