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MAFK Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6486169

MBS6486169 is an antibody targeting MAFK. Also known as MAFK, CT (v-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog K (Avian), MAFK Protein, FLJ32205, MGC71717, NFE2U, P18) (Azide free) (HRP). Reactive in Human. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
MAFK, Polyclonal Antibody
Full Product Name
MAFK, CT (v-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog K (Avian), MAFK Protein, FLJ32205, MGC71717, NFE2U, P18) (Azide free) (HRP)
Product Gene Name
anti-MAFK antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Purity/Purification
Purified by Protein G Affinity Chromatography.
Specificity
Recognizes human MAFK.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
63.633
OMIM
162100
3D Structure
ModBase 3D Structure for Q9UHD8
Immunogen
Synthetic peptide selected from the C-terminal region of human MAFK (KLH).
Conjugate
HRP
Note
Preservative Free

Product Categories/Family for anti-MAFK antibody

Antibodies; Transcription Factors
Applications Tested/Suitable for anti-MAFK antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes for anti-MAFK antibody
IHC: 1:50-1:100
ELISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.

Immunohistochemistry (IHC) of anti-MAFK antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Rabbit Anti-MAFK Polyclonal Antibody – Cat# MBS6486169

Western Blot (WB) of anti-MAFK antibody

The anti-SEPT9 Pab is used in Western blot to detect SEPT9 in Jurkat cell lysate.
Rabbit Anti-MAFK Polyclonal Antibody, Western Blot (WB) – Cat# MBS6486169

Immunohistochemistry (IHC) of anti-MAFK antibody

SEPT9 Antibody (A555) immunohistochemistry analysis in formalin fixed and paraffin embedded kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SEPT9 Antibody (A555) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-MAFK Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6486169

Flow Cytometry (FC/FACS) of anti-MAFK antibody

SEPT9 Antibody (A555) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-MAFK Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6486169

Product References and Citations for anti-MAFK antibody

1. Proc. Natl. Acad. Sci. USA 96:6428-6433(1999). 2. Cancer Res. 60: 4729-4734, 2000. 3. Oncogene 20: 5930-5939, 2001. 4. Kristi L, et al. Cancer Res., Dec 2009; 69: 9301-9305. (E-pub).

Other Notes

Small volumes of anti-MAFK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MAFK. It may not necessarily be applicable to this product.

NCBI GI #
116256489
NCBI GeneID
10801
NCBI Accession #
NP_006631.2
UniProt Primary Accession #
Q9UHD8
UniProt Related Accession #
Q9UHD8
GenBank Nucleotide #
NP_006631.2
NCBI Official Full Name
septin-9 isoform c
NCBI Official Synonym Full Names
septin 9
NCBI Official Symbol
SEPTIN9
NCBI Official Synonym Symbols
MSF; MSF1; NAPB; SEPT9; SINT1; PNUTL4; SeptD1; AF17q25
UniProt Protein Name
Septin-9
UniProt Synonym Protein Names
MLL septin-like fusion protein MSF-A; MLL septin-like fusion protein; Ovarian/Breast septin; Ov/Br septin; Septin D1
Protein Family
Transcription factor
UniProt Gene Name
SEPT9
UniProt Synonym Gene Names
KIAA0991; MSF; MLL septin-like fusion protein; Ov/Br septin
UniProt Entry Name
SEPT9_HUMAN
NCBI Protein Information
septin-9

NCBI Summary for MAFK

This gene is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Mar 2009]
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UniProt Comments for MAFK

Filament-forming cytoskeletal GTPase (). May play a role in cytokinesis (Potential). May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri.

Research Articles on MAFK

1. results indicate that SEPT9 promotes upregulation and both trafficking and secretion of MMPs near FAs, thus enhancing migration and invasion of breast cancer cells

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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