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MAGED4B Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6002790

This gene is a member of the MAGED gene family. It is expressed only in brain and ovary among normal tissues, and two transcript variants of this gene are specifically expressed in glioma cells among cancer cells. This gene and the other MAGED genes are… More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MAGED4B, Polyclonal Antibody
Full Product Name
MAGED4B, ID (MAGED4, KIAA1859, MAGED4A, MAGEE1, Melanoma-associated antigen D4, MAGE-D4 antigen, MAGE-E1 antigen)
Product Gene Name
Product Synonym Names
Anti -MAGED4B, ID (MAGED4, KIAA1859, MAGED4A, MAGEE1, Melanoma-associated antigen D4, MAGE-D4 antigen, MAGE-E1 antigen)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
81,378 Da
Chromosome Location
Chromosome: X; NC_000023.10 (51804923..51812368, complement). Location: Xp11
OMIM
300702
3D Structure
ModBase 3D Structure for Q96JG8
Immunogen
MAGED4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 327-357 amino acids from the Central region of human MAGED4B.
Applications Tested/Suitable for anti-MAGED4B antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-MAGED4B antibody
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50

Western Blot (WB) of anti-MAGED4B antibody

MAGED4B Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the MAGED4B antibody detected the MAGED4B protein (arrow).
Rabbit Anti-MAGED4B Polyclonal Antibody – Cat# MBS6002790

Immunohistochemistry (IHC) of anti-MAGED4B antibody

MAGED4B Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MAGED4B Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-MAGED4B Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6002790

Other Notes

Small volumes of anti-MAGED4B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MAGED4B purchase

MBS6002790 is an antibody targeting MAGED4B. Also known as MAGED4B, ID (MAGED4, KIAA1859, MAGED4A, MAGEE1, Melanoma-associated antigen D4, MAGE-D4 antigen, MAGE-E1 antigen). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MAGED4B. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
melanoma-associated antigen D4 isoform 3
NCBI Official Synonym Full Names
melanoma antigen family D, 4B
NCBI Official Symbol
MAGED4B
UniProt Protein Name
Melanoma-associated antigen D4
UniProt Synonym Protein Names
MAGE-D4 antigen; MAGE-E1 antigen
UniProt Gene Name
MAGED4
UniProt Synonym Gene Names
KIAA1859; MAGED4A; MAGEE1
UniProt Entry Name
MAGD4_HUMAN
NCBI Protein Information
melanoma-associated antigen D4; MAGE-D4 antigen; MAGE-E1 antigen

NCBI Summary for MAGED4B

This gene is a member of the MAGED gene family. It is expressed only in brain and ovary, and some transcript variants of this gene are specifically expressed in glioma cells. This gene is clustered with other MAGED genes on chromosome Xp11. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, May 2011]
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UniProt Comments for MAGED4B

MAGE-D4B: May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. 4 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: Xp11
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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