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MAP3K13 Antibody – Mouse Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9231365

Activates the JUN N-terminal pathway through activation of the MAP kinase kinase MAP2K7. Acts synergistically with PRDX3 to regulate the activation of NF-kappa-B in the cytosol. This activation is kinase-dependent and involves activating the IKK complex, the… More details

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Product Overview

Product Name
MAP3K13, Polyclonal Antibody
Full Product Name
MAP3K13 (LZK) Antibody (C-term)
Product Gene Name
anti-MAP3K13 antibody
Product Synonym Names
Mitogen-activated protein kinase kinase kinase 13; Leucine zipper-bearing kinase; Mixed lineage kinase; MLK; MAP3K13
Clonality
Polyclonal
Isotype
IgM
Clone Number
810CT26.6.1.1
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied in PBS with 0.09% (W/V) sodium azide.
Purity/Purification
Prepared by Euglobin precipitation followed by dialysis against PBS.
Specificity
Purified His-tagged MAP3K13 protein fragment was used to produced this monoclonal antibody.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Molecular Weight
108296 Da
OMIM
604915
3D Structure
ModBase 3D Structure for O43283
Antigen Source
Human
Antigen Region
52-305

Product Categories/Family for anti-MAP3K13 antibody

Signal Transduction
Applications Tested/Suitable for anti-MAP3K13 antibody
Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)

Western Blot (WB) of anti-MAP3K13 antibody

Dilution: WB - 1:500
Anti-MAP3K13 (LZK) Antibody (C-term) at 1:500 dilution + NCI-H460 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 110 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Mouse Anti-MAP3K13 Polyclonal Antibody – Cat# MBS9231365

Immunofluorescence (IF) of anti-MAP3K13 antibody

Dilution: IF - 1:100
Fluorescent image of NCI-H460 cells stained with MAP3K13 (LZK) Antibody (N-term). It was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgM at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
Mouse Anti-MAP3K13 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS9231365

Immunofluorescence (IF) of anti-MAP3K13 antibody

Dilution: IF - 1:100
Fluorescent image of NCI-H460 cells stained with MAP3K13 (LZK) Antibody (N-term). It was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgM at 1:400 dilution was used as the secondary antibody (green).
Mouse Anti-MAP3K13 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS9231365

of anti-MAP3K13 antibody

Dilution: IF - 1:100
Fluorescent image of NCI-H460 cells stained with MAP3K13 (LZK) Antibody (N-term). It was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgM at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Mouse Anti-MAP3K13 Polyclonal Antibody – Cat# MBS9231365

Western Blot (WB) of anti-MAP3K13 antibody

Dilution: WB - 1:1000
MAP3K13 (LZK) Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35mug/lane).This demonstrates the MAP3K13 (LZK) antibody detected the MAP3K13 (LZK) protein (arrow).
Mouse Anti-MAP3K13 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9231365

Product References and Citations for anti-MAP3K13 antibody

Ikeda A., et al. J. Biochem. 130:773-781(2001). Masaki M., et al. Eur. J. Biochem. 270:76-83(2003). Greenman C., et al. Nature 446:153-158(2007). Sakuma H., et al. J. Biol. Chem. 272:28622-28629(1997). Ota T., et al. Nat. Genet. 36:40-45(2004).

Other Notes

Small volumes of anti-MAP3K13 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MAP3K13 purchase

MBS9231365 is an antibody targeting MAP3K13. Also known as MAP3K13 (LZK) Antibody (C-term). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for MAP3K13. It may not necessarily be applicable to this product.

NCBI GI #
334085268
NCBI GeneID
9175
NCBI Accession #
NP_001229243.1
NCBI Related Accession #
NP_001229246.1; NP_004712.1
UniProt Primary Accession #
O43283
UniProt Related Accession #
O43283
GenBank Nucleotide #
NM_001242314.1
NCBI Official Full Name
mitogen-activated protein kinase kinase kinase 13 isoform 1
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase kinase 13
NCBI Official Symbol
MAP3K13
NCBI Official Synonym Symbols
LZK; MLK; MEKK13
UniProt Protein Name
Mitogen-activated protein kinase kinase kinase 13
UniProt Synonym Protein Names
Leucine zipper-bearing kinase; Mixed lineage kinase
Protein Family
Mitogen-activated protein kinase kinase kinase
UniProt Gene Name
MAP3K13
UniProt Synonym Gene Names
LZK; MLK
UniProt Entry Name
M3K13_HUMAN
NCBI Protein Information
mitogen-activated protein kinase kinase kinase 13

NCBI Summary for MAP3K13

The protein encoded by this gene is a member of serine/threonine protein kinase family. This kinase contains a dual leucine-zipper motif, and has been shown to form dimers/oligomers through its leucine-zipper motif. This kinase can phosphorylate and activate MAPK8/JNK, MAP2K7/MKK7, which suggests a role in the JNK signaling pathway. [provided by RefSeq, Jul 2008]
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UniProt Comments for MAP3K13

LZK: a TKL kinase of the MLK family. Synergizes with antioxidant protein-1 to activate NF-kappaB. Forms a functional signaling complex with JIP-1, a scaffold protein of the c-Jun NH(2)-terminal kinase pathway. This kinase can phosphorylate and activate JNK and MKK7. Contains a dual leucine-zipper motif, and has been shown to form dimers/oligomers through its leucine-zipper motif.

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; EC 2.7.11.25; Kinase, protein; TKL group; MLK family; LZK subfamily

Chromosomal Location of Human Ortholog: 3q27

Cellular Component: membrane; IkappaB kinase complex

Molecular Function: identical protein binding; protein serine/threonine kinase activity; protein binding; protein homodimerization activity; MAP kinase kinase kinase activity; metal ion binding; protein kinase binding; ATP binding

Biological Process: activation of MAPKK activity; protein amino acid autophosphorylation; JNK cascade; protein amino acid phosphorylation; activation of NF-kappaB transcription factor
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Research Articles on MAP3K13

1. Data indicate that miR-206's effect on Myc was mediated through MAP3K13.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MAP3K13 antibody

 Reference Product  PubMed Publications
 MAPK8IP1 antibody  >2 publications with MAP3K13 and MAPK8IP1
 PRDX3 antibody  >1 publications with MAP3K13 and PRDX3
 IKBKB antibody  >1 publications with MAP3K13 and IKBKB

Pathways associated with anti-MAP3K13 antibody

 Products by Pathway  Pathway Diagram
 Insulin Signaling Pathway antibodies  Insulin Signaling Pathway Diagram
 Integrated Breast Cancer Pathway antibodies  Integrated Breast Cancer Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 Signaling Events Mediated By VEGFR1 And VEGFR2 Pathway antibodies  Signaling Events Mediated By VEGFR1 And VEGFR2 Pathway Diagram

Diseases associated with anti-MAP3K13 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >2 publications with MAP3K13 and Neoplasms
 Cell Transformation, Neoplastic Antibodies  >1 publications with MAP3K13 and Cell Transformation, Neoplastic
 Anemia, Hemolytic Antibodies  >1 publications with MAP3K13 and Anemia, Hemolytic

Organs/Tissues associated with anti-MAP3K13 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >3 publications with MAP3K13 and Brain
 Nerve Antibodies  >1 publications with MAP3K13 and Nerve
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