• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

MAP3K3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS7607320

The mitogen-activated protein/ERK kinase kinase 3(MEKK3) is a member of a family of MEKK/Ste11 serine/threonine protein kinases. One function of MEKK3 is to serve as an upstream regulatory kinase in the mitogen-activated protein kinase cascade, however, it… More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Product Name
MAP3K3, Polyclonal Antibody
Full Product Name
Anti-MAP3K3 Antibody
Product Gene Name
Product Synonym Names
MAP3K3; MAPK/ERK kinase kinase 3; MAPKKK3; MEK kinase 3; MEKK 3; MEKK3
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid; PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Purity/Purification
>=95% as determined by SDS-PAGE
Immunogen Affinity Purified
Preparation and Storage
Store at -20 degree C for 24 months. Avoid repeated freeze/thaw cycles.
Molecular Weight
113 kDa
OMIM
602539
3D Structure
ModBase 3D Structure for Q99759
Immunogen
Mitogen-activated protein kinase kinase kinase 3
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-MAP3K3 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-MAP3K3 antibody
WB: 1:200-1:1000
IHC: 1:50-1:500

Immunohistochemistry (IHC) of anti-MAP3K3 antibody

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using MBS7607320 (MAP3K3 Antibody) at dilution of 1:100
Rabbit Anti-MAP3K3 Polyclonal Antibody – Cat# MBS7607320

Western Blot (WB) of anti-MAP3K3 antibody

HepG2 cells were subjected to SDS PAGE followed by western blot with MBS7607320 (MAP3K3 Antibody) at dilution of 1:300
Rabbit Anti-MAP3K3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS7607320

Other Notes

Small volumes of anti-MAP3K3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MAP3K3 purchase

MBS7607320 is an antibody targeting MAP3K3. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MAP3K3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
mitogen-activated protein kinase kinase kinase 3 isoform 2
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase kinase 3
NCBI Official Symbol
MAP3K3
NCBI Official Synonym Symbols
MEKK3; MAPKKK3
UniProt Protein Name
Mitogen-activated protein kinase kinase kinase 3
UniProt Synonym Protein Names
MAPK/ERK kinase kinase 3
UniProt Gene Name
MAP3K3
UniProt Synonym Gene Names
MAPKKK3; MEKK3; MEK kinase 3; MEKK 3
UniProt Entry Name
M3K3_HUMAN
NCBI Protein Information
mitogen-activated protein kinase kinase kinase 3

NCBI Summary for MAP3K3

This gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Show more

UniProt Comments for MAP3K3

MEKK3: a protein kinase of the STE11 family. Phosphorylated and activated by MLK3 and TAK1. Phosphorylates and activates MEK5.

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, STE; EC 2.7.11.25; Kinase, protein; STE group; STE11 family

Chromosomal Location of Human Ortholog: 17q23.3

Cellular Component: intracellular; cytosol

Molecular Function: MAP kinase kinase activity; protein binding; MAP kinase kinase kinase activity; metal ion binding; ATP binding; protein kinase activity

Biological Process: blood vessel development; positive regulation of I-kappaB kinase/NF-kappaB cascade; activation of MAPKK activity; activation of MAPK activity; protein amino acid autophosphorylation; MAPKKK cascade
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000