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MBL2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1758141

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MBL2, Polyclonal Antibody
Full Product Name
Anti-MBL2 Antibody Picoband
Product Gene Name
anti-MBL2 antibody
Product Synonym Names
D(4) dopamine receptor; mannose-binding lectin (protein C) 2; Mannose-binding protein C; MBP-C; Mannan-binding protein; RA-reactive factor P28A subunit; RARF/P28A; Mbl2
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Specificity
Found only in peripheral nervous system Schwann cells.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
Molecular Weight
26,144 Da
Chromosome Location
Chromosome: 10; NC_000010.10 (54525140..54531460, complement). Location: 10q11.2
OMIM
154545
3D Structure
ModBase 3D Structure for P11226
Immunogen
E coli-derived human MBL2 recombinant protein (Position: E21-I248).
Subcellular Localization
Secreted
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross-reactivity with other proteins.
Protein Function
Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages (By similarity).

Product Categories/Family for anti-MBL2 antibody

Primary Antibodies; Complement; Immunology; Innate Immunity
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-MBL2 antibody
Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-MBL2 antibody
WB: 0.1-0.25ug/ml, Human
FC/FACS (Fixed): 1-3ug/1x106 cells, Human
ELISA: 0.1-0.5ug/ml

Western Blot (WB) of anti-MBL2 antibody

Figure 1. Western blot analysis of MBL2 using anti-MBL2 antibody (MBS1758141).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MBL2 antigen affinity purified polyclonal antibody (#MBS1758141) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for MBL2 at approximately 26 kDa. The expected band size for MBL2 is at 26 kDa.
Rabbit Anti-MBL2 Polyclonal Antibody – Cat# MBS1758141

Flow Cytometry (FC/FACS) of anti-MBL2 antibody

Figure 2. Flow Cytometry analysis of HepG2 cells using anti-MBL2 antibody (MBS1758141).
Overlay histogram showing HepG2 cells stained with MBS1758141 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-MBL2 Antibody (MBS1758141, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (MBS177405, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Rabbit Anti-MBL2 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1758141

Product References and Citations for anti-MBL2 antibody

1. Bodamer, O. A., Mitterer, G., Maurer, W., Pollak, A., Mueller, M. W., Schmidt, W. M. Evidence for an association between mannose-binding lectin 2 (MBL2) gene polymorphisms and pre-term birth. Genet. Med. 8: 518-524, 2006.2. Buranawuti, K., Boyle, M. P., Cheng, S., Steiner, L. L., McDougal, K., Fallin, M. D., Merlo, C., Zeitlin, P. L., Rosenstein, B. J., Mogayzel, P. J., Jr., Wang, X., Cutting, G. R. Variants in mannose-binding lectin and tumour necrosis factor alpha affect survival in cystic fibrosis. J. Med. Genet. 44: 209-214, 2007. 3. Davies, J., Neth, O., Alton, E., Klein, N., Turner, M. Differential binding of mannose-binding lectin to respiratory pathogens in cystic fibrosis. Lancet 355: 1885-1886, 2000.

Other Notes

Small volumes of anti-MBL2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MBL2 purchase

MBS1758141 is an antibody targeting MBL2. Also known as Anti-MBL2 Antibody Picoband. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MBL2. It may not necessarily be applicable to this product.

NCBI GI #
4557739
NCBI GeneID
4153
NCBI Accession #
NP_000233.1
UniProt Primary Accession #
P11226
UniProt Secondary Accession #
Q4VB12; Q4VB13; Q86SI4; Q96KE4; Q96TF7; Q96TF8; Q96TF9
UniProt Related Accession #
P11226
GenBank Nucleotide #
NM_000242.2
NCBI Official Full Name
mannose-binding protein C
NCBI Official Synonym Full Names
mannose-binding lectin (protein C) 2, soluble
NCBI Official Symbol
MBL2
NCBI Official Synonym Symbols
MBL; MBP; MBP1; MBPD; MBL2D; MBP-C; COLEC1; HSMBPC
UniProt Protein Name
Mannose-binding protein C
UniProt Synonym Protein Names
Collectin-1; MBP1; Mannan-binding protein; Mannose-binding lectin
Protein Family
Mannose-binding protein
UniProt Gene Name
MBL2
UniProt Synonym Gene Names
COLEC1; MBL; MBP-C
UniProt Entry Name
MBL2_HUMAN
NCBI Protein Information
mannose-binding protein C; collectin-1; mannan-binding lectin; mannose-binding lectin 2, soluble (opsonic defect); mannose-binding lectin (protein C) 2, soluble (opsonic defect)

NCBI Summary for MBL2

This gene encodes the soluble mannose-binding lectin or mannose-binding protein found in serum. The protein encoded belongs to the collectin family and is an important element in the innate immune system. The protein recognizes mannose and N-acetylglucosamine on many microorganisms, and is capable of activating the classical complement pathway. Deficiencies of this gene have been associated with susceptibility to autoimmune and infectious diseases. [provided by RefSeq, Jul 2008]
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UniProt Comments for MBL2

MBL2: Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: extracellular space; cell surface; collagen; extracellular region

Molecular Function: mannose binding; protein binding; calcium ion binding; calcium-dependent protein binding; receptor binding

Biological Process: defense response to Gram-positive bacterium; killing by host of symbiont cells; positive regulation of phagocytosis; defense response to bacterium; acute-phase response; innate immune response; response to oxidative stress; opsonization; complement activation, lectin pathway; complement activation, classical pathway; negative regulation of viral reproduction; complement activation

Disease: Mannose-binding Protein Deficiency
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Research Articles on MBL2

1. The data do not support a role of MBL deficiency on susceptibility to pneumococcal community-acquired pneumonia or to invasive pneumococcal disease

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MBL2 antibody

 Reference Product  PubMed Publications
 MASP2 antibody  >31 publications with MBL2 and MASP2
 MASP1 antibody  >7 publications with MBL2 and MASP1
 PTPRC antibody  >1 publications with MBL2 and PTPRC

Pathways associated with anti-MBL2 antibody

 Products by Pathway  Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement Cascade Pathway antibodies  Complement Cascade Pathway Diagram
 Creation Of C4 And C2 Activators Pathway antibodies  Creation Of C4 And C2 Activators Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Initial Triggering Of Complement Pathway antibodies  Initial Triggering Of Complement Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Lectin Pathway Of Complement Activation antibodies  Lectin Pathway Of Complement Activation Diagram
 Phagosome Pathway antibodies  Phagosome Pathway Diagram
 Phagosome Pathway antibodies  Phagosome Pathway Diagram

Diseases associated with anti-MBL2 antibody

 Disease Name  Pubmed Publications
 Infection Antibodies  >172 publications with MBL2 and Infection
 Inflammation Antibodies  >73 publications with MBL2 and Inflammation
 Neoplasms Antibodies  >45 publications with MBL2 and Neoplasms
 Liver Diseases Antibodies  >29 publications with MBL2 and Liver Diseases
 Fibrosis Antibodies  >24 publications with MBL2 and Fibrosis
 HIV Infections Antibodies  >22 publications with MBL2 and HIV Infections
 Heart Diseases Antibodies  >18 publications with MBL2 and Heart Diseases
 Necrosis Antibodies  >17 publications with MBL2 and Necrosis
 Premature Birth Antibodies  >12 publications with MBL2 and Premature Birth
 Disease Models, Animal Antibodies  >11 publications with MBL2 and Disease Models, Animal

Organs/Tissues associated with anti-MBL2 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >43 publications with MBL2 and Liver
 Mixed Antibodies  >3 publications with MBL2 and Mixed
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