Rabbit Anti-MDMX/MDM4 Polyclonal Antibody – Cat# MBS1758243

Figure 1. Western blot analysis of MDMX/MDM4 using anti-MDMX/MDM4 antibody (MBS1758243).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MDMX/MDM4 antigen affinity purified polyclonal antibody (#MBS1758243) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for MDMX/MDM4 at approximately 55 kDa. The expected band size for MDMX/MDM4 is at 55 kDa.

MDMX/MDM4 Antibody – Rabbit Polyclonal

Name: MDMX/MDM4 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1758243
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1758243

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring… More details

$210 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

MDMX/MDM4, Polyclonal Antibody

Full Product Name

Anti-MDMX/MDM4 Antibody Picoband

Product Gene Name

anti-MDMX/MDM4 antibody

Product Synonym Names

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2; Probable global transcription activator SNF2L2; ATP-dependent helicase SMARCA2; BRG1-associated factor 190B; BAF190B; Protein brahma homolog; hBRM; SNF2-alpha; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2; SMARCA2; BAF190B; BRM; SNF2A; SNF2L2

Clonality

Polyclonal

Isotype

Rabbit IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purity/Purification

Immunogen affinity purified.

Specificity

Detected in milk (at protein level).

Preparation and Storage

At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.

Molecular Weight

54,864 Da

Chromosome Location

Chromosome: 1; NC_000001.10 (204485507..204527248). Location: 1q32

OMIM

602704

3D Structure

ModBase 3D Structure for O15151

Immunogen

E coli-derived human MDMX/MDM4 recombinant protein (Position: A8-A490).

Subcellular Localization

Nucleus

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.

Cross Reactivity

No cross-reactivity with other proteins.

Protein Function

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Binds DNA non-specifically. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.

Product Categories/Family for anti-MDMX/MDM4 antibody

Primary Antibodies; Cancer; Energy Metabolism; Energy Transfer Pathways; Metabolic Signaling Pathways; Metabolism; Pathways and Processes; Signal Transduction

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-MDMX/MDM4 antibody

Western Blot (WB), ELISA (EIA)

Application Notes for anti-MDMX/MDM4 antibody

WB: 0.25-0.5ug/ml, Human
ELISA: 0.1-0.5ug/ml

Product References and Citations for anti-MDMX/MDM4 antibody

1. "Entrez Gene: MDM4 Mdm4, transformed 3T3 cell double minute 4, p53 binding protein (mouse)".2. Kadakia M, Brown TL, McGorry MM, Berberich SJ (Dec 2002). "MdmX inhibits Smad transactivation". Oncogene21 (57): 8776-85.3. Shvarts A, Bazuine M, Dekker P, Ramos YF, Steegenga WT, Merckx G, van Ham RC, van der Houven van Oordt W, van der Eb AJ, Jochemsen AG (Sep 1997). "Isolation and identification of the human homolog of a new p53-binding protein, Mdmx". Genomics 43 (1): 34-42.

Other Notes

Small volumes of anti-MDMX/MDM4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MDMX/MDM4 purchase

MBS1758243 is an antibody targeting MDMX/MDM4. Also known as Anti-MDMX/MDM4 Antibody Picoband. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MDMX/MDM4. It may not necessarily be applicable to this product.

NCBI GI #

323510634

NCBI GeneID

4194

NCBI Accession #

NP_001191100.1

UniProt Primary Accession #

O15151

UniProt Secondary Accession #

Q2M2Y2; Q32SL2; Q6GS18; Q8IV83

UniProt Related Accession #

O15151

GenBank Nucleotide #

NM_001204171.1

NCBI Official Full Name

protein Mdm4 isoform 2

NCBI Official Synonym Full Names

Mdm4 p53 binding protein homolog (mouse)

NCBI Official Symbol

MDM4

NCBI Official Synonym Symbols

HDMX; MDMX; MRP1

UniProt Protein Name

Protein Mdm4

UniProt Synonym Protein Names

Double minute 4 protein; Mdm2-like p53-binding protein; Protein Mdmx; p53-binding protein Mdm4

UniProt Gene Name

MDM4

UniProt Synonym Gene Names

MDMX

UniProt Entry Name

MDM4_HUMAN

NCBI Protein Information

protein Mdm4; protein Mdmx; MDM4-related protein 1; mdm2-like p53-binding protein; double minute 4, human homolog of; p53-binding protein

NCBI Summary for MDMX/MDM4

This gene encodes a nuclear protein that contains a p53 binding domain at the N-terminus and a RING finger domain at the C-terminus, and shows structural similarity to p53-binding protein MDM2. Both proteins bind the p53 tumor suppressor protein and inhibit its activity, and have been shown to be overexpressed in a variety of human cancers. However, unlike MDM2 which degrades p53, this protein inhibits p53 by binding its transcriptional activation domain. This protein also interacts with MDM2 protein via the RING finger domain, and inhibits the latter's degradation. So this protein can reverse MDM2-targeted degradation of p53, while maintaining suppression of p53 transactivation and apoptotic functions. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Feb 2011]

UniProt Comments for MDMX/MDM4

MDM4: Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions. Interacts with MDM2, TP53, TP73 and USP2. Found in a trimeric complex with UPB2, MDM2 and MDM4. Interacts (phosphorylated) with YWHAG; negatively regulates MDM4 activity toward TP53. Down-regulated by cisplatin. Expressed in all tissues tested with high levels in thymus. Belongs to the MDM2/MDM4 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Ubiquitin ligase; EC 6.3.2.-; Ligase

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; enzyme binding; zinc ion binding

Biological Process: cell proliferation; negative regulation of cell proliferation; protein stabilization; DNA damage response, signal transduction by p53 class mediator; G0 to G1 transition; positive regulation of cell proliferation; protein complex assembly; negative regulation of transcription from RNA polymerase II promoter; negative regulation of protein catabolic process; negative regulation of apoptosis

Research Articles on MDMX/MDM4

1. quantify the expression of MDM4 and MDM2 mRNA and protein in human fetal retinae, primary retinoblastomas, retinoblastoma cell lines and several independent orthotopic retinoblastoma xenografts

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MDMX/MDM4 antibody

Reference Product	PubMed Publications
MDM2 antibody	>219 publications with MDMX/MDM4 and MDM2
TP53 antibody	>58 publications with MDMX/MDM4 and TP53
RB1 antibody	>13 publications with MDMX/MDM4 and RB1
ATM antibody	>10 publications with MDMX/MDM4 and ATM
CASP3 antibody	>7 publications with MDMX/MDM4 and CASP3
USP7 antibody	>5 publications with MDMX/MDM4 and USP7
E2F1 antibody	>3 publications with MDMX/MDM4 and E2F1
AKT1 antibody	>2 publications with MDMX/MDM4 and AKT1
EP300 antibody	>1 publications with MDMX/MDM4 and EP300
CHEK2 antibody	>1 publications with MDMX/MDM4 and CHEK2

Pathways associated with anti-MDMX/MDM4 antibody

Products by Pathway	Pathway Diagram
Signaling Pathways In Glioblastoma antibodies	Signaling Pathways In Glioblastoma Diagram
P53 Pathway antibodies	P53 Pathway Diagram
P53 Signaling Pathway antibodies	P53 Signaling Pathway Diagram
P53 Signaling Pathway antibodies	P53 Signaling Pathway Diagram

Diseases associated with anti-MDMX/MDM4 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>128 publications with MDMX/MDM4 and Neoplasms
Neoplasms, Experimental Antibodies	>15 publications with MDMX/MDM4 and Neoplasms, Experimental
Nervous System Diseases Antibodies	>14 publications with MDMX/MDM4 and Nervous System Diseases
Cell Transformation, Neoplastic Antibodies	>13 publications with MDMX/MDM4 and Cell Transformation, Neoplastic
Breast Neoplasms Antibodies	>13 publications with MDMX/MDM4 and Breast Neoplasms
Adenocarcinoma Antibodies	>9 publications with MDMX/MDM4 and Adenocarcinoma
Glioblastoma Antibodies	>7 publications with MDMX/MDM4 and Glioblastoma
Ovarian Neoplasms Antibodies	>4 publications with MDMX/MDM4 and Ovarian Neoplasms
Heart Diseases Antibodies	>4 publications with MDMX/MDM4 and Heart Diseases
Liver Diseases Antibodies	>3 publications with MDMX/MDM4 and Liver Diseases

Organs/Tissues associated with anti-MDMX/MDM4 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>14 publications with MDMX/MDM4 and Brain
Embryonic Tissue Antibodies	>11 publications with MDMX/MDM4 and Embryonic Tissue
Bone Antibodies	>10 publications with MDMX/MDM4 and Bone
Eye Antibodies	>9 publications with MDMX/MDM4 and Eye
Liver Antibodies	>8 publications with MDMX/MDM4 and Liver
Lung Antibodies	>8 publications with MDMX/MDM4 and Lung
Blood Antibodies	>8 publications with MDMX/MDM4 and Blood
Bone Marrow Antibodies	>7 publications with MDMX/MDM4 and Bone Marrow
Muscle Antibodies	>6 publications with MDMX/MDM4 and Muscle
Thyroid Antibodies	>5 publications with MDMX/MDM4 and Thyroid