Rabbit Anti-METAP2 Polyclonal Antibody – Cat# MBS6487866

Western Blot analysis of 293 cell line lysates (35ug/lane) using MBS626620 (arrow) was detected using the purified Pab.

METAP2 Antibody – Rabbit Polyclonal

Name: METAP2 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS6487866
Price: 1060 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6487866

MBS6487866 is an antibody targeting METAP2. Also known as METAP2, NT (Methionine Aminopeptidase 2, MetAP 2, MAP 2, Peptidase M 2, Initiation Factor 2-associated 67kD Glycoprotein, p67eIF2) (AP). Reactive in Human. Host: Rabbit. More details

$1060 / 0.2 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

METAP2, Polyclonal Antibody

Full Product Name

METAP2, NT (Methionine Aminopeptidase 2, MetAP 2, MAP 2, Peptidase M 2, Initiation Factor 2-associated 67kD Glycoprotein, p67eIF2) (AP)

Product Gene Name

anti-METAP2 antibody

Product Synonym Gene Name

AMP2

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

Purity/Purification

Purified by Protein A/G Affinity Chromatography.

Specificity

Recognizes human METAP2.

Preparation and Storage

Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Molecular Weight

53kDa

3D Structure

ModBase 3D Structure for P50579

Immunogen

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 10-40 in the N-terminal region of human METAP2, UniProt Accession #P50579; NP_006829.

Conjugate

Note

Preservative Free

Product Categories/Family for anti-METAP2 antibody

Antibodies; Amino Acids

Applications Tested/Suitable for anti-METAP2 antibody

Western Blot (WB), ELISA (EIA)

Application Notes for anti-METAP2 antibody

WB: 1:100-1:500
ELISA: 1:1000
Applications are based on unconjugated antibody.

Product References and Citations for anti-METAP2 antibody

1. Wang, J., et al., Biochemistry 42(17):5035-5042 (2003). 2. Datta, R., et al., Exp. Cell Res. 283(2):237-246 (2003). 3. Endo, H., et al., J. Biol. Chem. 277(29):26396-26402 (2002). 4. Kanno, T., et al., Lab. Invest. 82(7):893-901 (2002). 5. Li, X., et al., Biochem. Biophys. Res. Commun. 227(1):152-159 (1996).

Other Notes

Small volumes of anti-METAP2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for METAP2. It may not necessarily be applicable to this product.

NCBI GI #

5803092

NCBI GeneID

10988

NCBI Accession #

NP_006829

UniProt Primary Accession #

P50579

UniProt Related Accession #

P50579

GenBank Nucleotide #

NM_006838

NCBI Official Full Name

methionine aminopeptidase 2 isoform 1

NCBI Official Synonym Full Names

methionyl aminopeptidase 2

NCBI Official Symbol

METAP2

NCBI Official Synonym Symbols

MAP2; MNPEP; p67eIF2

UniProt Protein Name

Methionine aminopeptidase 2

UniProt Synonym Protein Names

Initiation factor 2-associated 67 kDa glycoproteinUniRule annotationManual assertion according to rulesiHAMAP-Rule:MF_03175; p67UniRule annotationManual assertion according to rulesiHAMAP-Rule:MF_03175; p67eIF2UniRule annotationManual assertion according to rulesiHAMAP-Rule:MF_03175; Peptidase MUniRule annotationManual assertion according to rulesiHAMAP-Rule:MF_03175

UniProt Gene Name

METAP2

UniProt Synonym Gene Names

MNPEP; P67EIF2; MAP 2

NCBI Protein Information

methionine aminopeptidase 2

NCBI Summary for METAP2

The protein encoded by this gene is a member of the methionyl aminopeptidase family. The encoded protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent proteins. Increased expression of this gene is associated with various forms of cancer, and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. Inhibitors of this gene have also been shown to be effective for the treatment of obesity. A pseudogene of this gene is located on chromosome 2. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]

UniProt Comments for METAP2

Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo.

Research Articles on METAP2

1. Results show that methionine aminopeptidase-2 (MetAP2) regulates angiogenesis in glioblastoma and identify MetAP2-specific substrates that may serve as candidates for clinical assay development.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-METAP2 antibody

Reference Product	PubMed Publications
METAP1 antibody	>20 publications with METAP2 and METAP1
S100A4 antibody	>2 publications with METAP2 and S100A4

Pathways associated with anti-METAP2 antibody

Products by Pathway	Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Diseases Associated With Visual Transduction Pathway antibodies	Diseases Associated With Visual Transduction Pathway Diagram
Inactivation, Recovery And Regulation Of The Phototransduction Cascade Pathway antibodies	Inactivation, Recovery And Regulation Of The Phototransduction Cascade Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram
The Phototransduction Cascade Pathway antibodies	The Phototransduction Cascade Pathway Diagram
Visual Phototransduction Pathway antibodies	Visual Phototransduction Pathway Diagram

Diseases associated with anti-METAP2 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>33 publications with METAP2 and Neoplasms
Disease Models, Animal Antibodies	>13 publications with METAP2 and Disease Models, Animal
Liver Diseases Antibodies	>9 publications with METAP2 and Liver Diseases
Liver Neoplasms Antibodies	>8 publications with METAP2 and Liver Neoplasms
Adenocarcinoma Antibodies	>6 publications with METAP2 and Adenocarcinoma
Liver Neoplasms, Experimental Antibodies	>5 publications with METAP2 and Liver Neoplasms, Experimental
Inflammation Antibodies	>4 publications with METAP2 and Inflammation
Carcinoma, Hepatocellular Antibodies	>3 publications with METAP2 and Carcinoma, Hepatocellular
Breast Neoplasms Antibodies	>3 publications with METAP2 and Breast Neoplasms
Neoplasm Metastasis Antibodies	>2 publications with METAP2 and Neoplasm Metastasis

Organs/Tissues associated with anti-METAP2 antibody

Organ/Tissue Name	Pubmed Publications
Vascular Antibodies	>27 publications with METAP2 and Vascular
Blood Antibodies	>15 publications with METAP2 and Blood
Liver Antibodies	>11 publications with METAP2 and Liver
Umbilical Cord Antibodies	>6 publications with METAP2 and Umbilical Cord
Embryonic Tissue Antibodies	>5 publications with METAP2 and Embryonic Tissue
Lung Antibodies	>4 publications with METAP2 and Lung
Lymph Antibodies	>3 publications with METAP2 and Lymph
Brain Antibodies	>3 publications with METAP2 and Brain
Prostate Antibodies	>2 publications with METAP2 and Prostate
Connective Tissue Antibodies	>2 publications with METAP2 and Connective Tissue