Rabbit Anti-MFI2 Polyclonal Antibody – Cat# MBS6320520

Western Blot analysis in MDA-MB231 cell line lysates (35ug/lane) usingMBS6001624. This demonstrates that MBS6001624 detected the MFI2 protein (arrow).

MFI2 Antibody – Rabbit Polyclonal

Name: MFI2 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS6320520
Price: 1060 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6320520

$1060 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

MFI2, Polyclonal Antibody

Full Product Name

MFI2, CT (MFI2, MAP97, Melanotransferrin, Melanoma-associated antigen p97, CD228) (MaxLight 490)

Product Gene Name

anti-MFI2 antibody

Product Synonym Names

MFI2; MAP97; Melanotransferrin; Melanoma-associated antigen p97; CD228

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

Purity/Purification

Purified by Protein A Affinity Chromatography.

Specificity

Recognizes human MFI2.

Preparation and Storage

Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Molecular Weight

80,215 Da

OMIM

155750

3D Structure

ModBase 3D Structure for P08582

Immunogen

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 627-655 in the C-terminal region of human MFI2, UniProt Accession #NP_005920.2; P08582. Species sequence homology: mouse and rabbit

Conjugate

MaxLight490

Note

Preservative Free

Product Categories/Family for anti-MFI2 antibody

Antibodies; CD Markers

Applications Tested/Suitable for anti-MFI2 antibody

Western Blot (WB), FLISA

Application Notes for anti-MFI2 antibody

WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.

Product References and Citations for anti-MFI2 antibody

1. Wang, M., et al. J. Biol. Chem. 284(48):33377-33383(2009). 2. Farnaud, S., et al. Int. J. Biochem. Cell Biol. 40(12):2739-2745(2008). 3. Suryo Rahmanto, Y., et al. Carcinogenesis 28(10):2172-2183(2007). 4. Suryo Rahmanto, Y., et al. Oncogene 26(42):6113-6124(2007). 5. Michaud-Levesque, J., et al. Biol. Chem. 388(7):747-754(2007).

Other Notes

Small volumes of anti-MFI2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MFI2 purchase

MBS6320520 is an antibody targeting MFI2. Also known as MFI2, CT (MFI2, MAP97, Melanotransferrin, Melanoma-associated antigen p97, CD228) (MaxLight 490). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MFI2. It may not necessarily be applicable to this product.

NCBI GI #

134244281

NCBI GeneID

4241

NCBI Accession #

NP_005920.2

UniProt Primary Accession #

P08582

UniProt Secondary Accession #

Q9BQE2

UniProt Related Accession #

P08582

GenBank Nucleotide #

NM_005929.5

NCBI Official Full Name

melanotransferrin isoform 1

NCBI Official Synonym Full Names

antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5

NCBI Official Symbol

MFI2

NCBI Official Synonym Symbols

MTF1; CD228; MAP97

UniProt Protein Name

Melanotransferrin

UniProt Synonym Protein Names

Melanoma-associated antigen p97

UniProt Gene Name

MFI2

UniProt Synonym Gene Names

MAP97

UniProt Entry Name

TRFM_HUMAN

NCBI Protein Information

melanotransferrin; membrane-bound transferrin-like protein; melanoma-associated antigen p97, isoform 2

NCBI Summary for MFI2

The protein encoded by this gene is a cell-surface glycoprotein found on melanoma cells. The protein shares sequence similarity and iron-binding properties with members of the transferrin superfamily. The importance of the iron binding function has not yet been identified. This gene resides in the same region of chromosome 3 as members of the transferrin superfamily. Alternative splicing results in two transcript variants. [provided by RefSeq, Jul 2008]

UniProt Comments for MFI2

MFI2: Involved in iron cellular uptake. Seems to be internalized and then recycled back to the cell membrane. Binds a single atom of iron per subunit. Could also bind zinc. Belongs to the transferrin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 3q28-q29

Cellular Component: cell surface; anchored to plasma membrane; integral to plasma membrane

Molecular Function: protein binding; ferric iron binding; iron ion binding

Biological Process: cellular iron ion homeostasis; regulation of cell growth; regulation of cell proliferation

Research Articles on MFI2

1. PC3 tumors are sustained by a small number of tumor-initiating cells with stem-like characteristics, including strong self-renewal and pro-angiogenic capability and marked by the expression pattern FAM65Bhigh/MFI2low/LEF1low.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-MFI2 antibody

Disease Name	Pubmed Publications
Melanoma Antibodies	>4 publications with MFI2 and Melanoma
Neoplasms Antibodies	>3 publications with MFI2 and Neoplasms
Nervous System Diseases Antibodies	>1 publications with MFI2 and Nervous System Diseases
Movement Disorders Antibodies	>1 publications with MFI2 and Movement Disorders
Hemorrhage Antibodies	>1 publications with MFI2 and Hemorrhage
Breast Neoplasms Antibodies	>1 publications with MFI2 and Breast Neoplasms
Congenital Abnormalities Antibodies	>1 publications with MFI2 and Congenital Abnormalities

Organs/Tissues associated with anti-MFI2 antibody

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>1 publications with MFI2 and Bone
Embryonic Tissue Antibodies	>1 publications with MFI2 and Embryonic Tissue
Kidney Antibodies	>1 publications with MFI2 and Kidney
Intestine Antibodies	>1 publications with MFI2 and Intestine
Prostate Antibodies	>1 publications with MFI2 and Prostate
Eye Antibodies	>1 publications with MFI2 and Eye
Brain Antibodies	>1 publications with MFI2 and Brain