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MID2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6320782

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in… More details

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Product Overview

Online Datasheet in PDF
Product Name
MID2, Polyclonal Antibody
Full Product Name
MID2, CT (MID2, FXY2, RNF60, TRIM1, Probable E3 ubiquitin-protein ligase MID2, Midin-2, Midline defect 2, Midline-2, RING finger protein 60, Tripartite motif-containing protein 1) (Azide free) (HRP)
Product Gene Name
anti-MID2 antibody
Product Synonym Names
MID2; FXY2; RNF60; TRIM1; Probable E3 ubiquitin-protein ligase MID2; Midin-2; Midline defect 2; Midline-2; RING finger protein 60; Tripartite motif-containing protein 1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
79,865 Da
OMIM
gene 300928
3D Structure
ModBase 3D Structure for Q9UJV3
Immunogen
MID2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 669-698 amino acids from the C-terminal region of human MID2.
Conjugate
HRP
Note
Preservative Free

Product Categories/Family for anti-MID2 antibody

Antibodies; Transcription Factors; TRIM (Tripartite Motif)
Applications Tested/Suitable for anti-MID2 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes for anti-MID2 antibody
IHC: 1:10-50
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-MID2 antibody

MID2 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the MID2 antibody detected the MID2 protein (arrow).
Rabbit Anti-MID2 Polyclonal Antibody – Cat# MBS6320782

Immunohistochemistry (IHC) of anti-MID2 antibody

MID2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MID2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-MID2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6320782

Product References and Citations for anti-MID2 antibody

Lim, J., et al. Cell 125(4):801-814(2006). Jehee, F.S., et al. Am. J. Med. Genet. A 139(3):221-226(2005). Yap, M.W., et al. Proc. Natl. Acad. Sci. U.S.A. 101(29):10786-10791(2004). Short, K.M., et al. BMC Cell Biol. 3, 1 (2002): Reymond, A., et al. EMBO J. 20(9):2140-2151(2001).

Other Notes

Small volumes of anti-MID2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MID2 purchase

MBS6320782 is an antibody targeting MID2. Also known as MID2, CT (MID2, FXY2, RNF60, TRIM1, Probable E3 ubiquitin-protein ligase MID2, Midin-2, Midline defect 2, Midline-2, RING finger protein 60, Tripartite motif-containing protein 1) (Azide free) (HRP). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MID2. It may not necessarily be applicable to this product.

NCBI GI #
223890257
NCBI GeneID
11043
NCBI Accession #
NP_036348.2
NCBI Related Accession #
NP_438112.2
UniProt Primary Accession #
Q9UJV3
UniProt Secondary Accession #
Q5JYF5; Q8WWK1; Q9UJR9; A6NEL8; A6PVI5
UniProt Related Accession #
Q9UJV3
GenBank Nucleotide #
NM_012216.3
NCBI Official Full Name
probable E3 ubiquitin-protein ligase MID2 isoform 1
NCBI Official Synonym Full Names
midline 2
NCBI Official Symbol
MID2
NCBI Official Synonym Symbols
FXY2; RNF60; TRIM1; MRX101
UniProt Protein Name
Probable E3 ubiquitin-protein ligase MID2
UniProt Synonym Protein Names
Midin-2; Midline defect 2; Midline-2; RING finger protein 60; Tripartite motif-containing protein 1
Protein Family
Cell wall integrity sensor
UniProt Gene Name
MID2
UniProt Synonym Gene Names
FXY2; RNF60; TRIM1
UniProt Entry Name
TRIM1_HUMAN
NCBI Protein Information
probable E3 ubiquitin-protein ligase MID2; midin 2; midin-2; midline-2; midline defect 2; RING finger protein 60; tripartite motif protein 1; tripartite motif-containing protein 1

UniProt Comments for MID2

MID2: is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Feb 2009]

Protein type: Microtubule-binding; EC 6.3.2.-; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: Xq22.3

Cellular Component: microtubule; cytoplasm

Molecular Function: protein homodimerization activity; zinc ion binding; protein heterodimerization activity; microtubule binding; phosphoprotein binding; ligase activity

Biological Process: negative regulation of viral transcription; positive regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of virion penetration into host cell; protein ubiquitination; innate immune response; positive regulation of transcription factor activity; activation of NF-kappaB transcription factor

Disease: Mental Retardation, X-linked 101
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Research Articles on MID2

1. A novel missense mutation (c.1040G>A, p.Arg347Gln) was reported in MID2, which encodes ubiquitin ligase E3, as the likely cause of X-linked mental retardation in a large kindred.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MID2 antibody

 Reference Product  PubMed Publications
 MID1 antibody  >13 publications with MID2 and MID1

Diseases associated with anti-MID2 antibody

 Disease Name  Pubmed Publications
 Metabolic Diseases Antibodies  >4 publications with MID2 and Metabolic Diseases
 Nervous System Diseases Antibodies  >2 publications with MID2 and Nervous System Diseases
 Lung Diseases Antibodies  >2 publications with MID2 and Lung Diseases
 Heart Diseases Antibodies  >1 publications with MID2 and Heart Diseases
 Urogenital Abnormalities Antibodies  >1 publications with MID2 and Urogenital Abnormalities
 Ovarian Neoplasms Antibodies  >1 publications with MID2 and Ovarian Neoplasms
 Mental Disorders Antibodies  >1 publications with MID2 and Mental Disorders
 Eye Diseases Antibodies  >1 publications with MID2 and Eye Diseases
 Fibrosis Antibodies  >1 publications with MID2 and Fibrosis

Organs/Tissues associated with anti-MID2 antibody

 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >3 publications with MID2 and Embryonic Tissue
 Eye Antibodies  >1 publications with MID2 and Eye
 Lung Antibodies  >1 publications with MID2 and Lung
 Muscle Antibodies  >1 publications with MID2 and Muscle
 Brain Antibodies  >1 publications with MID2 and Brain
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