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MILK2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6320843

MBS6320843 is an antibody targeting MILK2. Also known as MILK2, CT (MICALL2, MICAL-like protein 2) (PE). Reactive in Human. Host: Rabbit. More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MILK2, Polyclonal Antibody
Full Product Name
MILK2, CT (MICALL2, MICAL-like protein 2) (PE)
Product Gene Name
anti-MILK2 antibody
Product Synonym Gene Name
MICALL2
Product Synonym Names
MICALL2; MICAL-like protein 2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
74,527 Da
3D Structure
ModBase 3D Structure for Q8IY33
Immunogen
MILK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acids 846-876 from the C-terminal region of human MILK2.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-MILK2 antibody

Antibodies; Proteins
Applications Tested/Suitable for anti-MILK2 antibody
Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-MILK2 antibody
FC/FACS: 1:10-50
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-MILK2 antibody

MILK2 Antibody (C-term) western blot analysis in HL-60,CEM cell line lysates (35ug/lane).This demonstrates the MILK2 antibody detected the MILK2 protein (arrow).
Rabbit Anti-MILK2 Polyclonal Antibody – Cat# MBS6320843

Immunohistochemistry (IHC) of anti-MILK2 antibody

MILK2 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MILK2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-MILK2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6320843

Flow Cytometry (FC/FACS) of anti-MILK2 antibody

MILK2 Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-MILK2 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6320843

Product References and Citations for anti-MILK2 antibody

Kanda, I., et al. Oncogene 27(12):1687-1695(2008). Terai, T., et al. Mol. Biol. Cell 17(5):2465-2475(2006). Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003). Terman, J.R., et al. Cell 109(7):887-900(2002).

Other Notes

Small volumes of anti-MILK2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MILK2. It may not necessarily be applicable to this product.

NCBI GI #
33667117
NCBI GeneID
79778
NCBI Accession #
NP_891554.1
UniProt Primary Accession #
Q8IY33
UniProt Secondary Accession #
Q7RTP4; Q7Z655; Q8TEQ4; Q9H5F9; D3YTD2
UniProt Related Accession #
Q8IY33
GenBank Nucleotide #
NM_182924.3
NCBI Official Full Name
MICAL-like protein 2
NCBI Official Synonym Full Names
MICAL like 2
NCBI Official Symbol
MICALL2
NCBI Official Synonym Symbols
JRAB; MICAL-L2
UniProt Protein Name
MICAL-like protein 2
UniProt Synonym Protein Names
Junctional Rab13-binding protein; Molecule interacting with CasL-like 2; MICAL-L2
UniProt Gene Name
MICALL2
UniProt Synonym Gene Names
JRAB; MICAL-L2
NCBI Protein Information
MICAL-like protein 2

UniProt Comments for MILK2

Effector of small Rab GTPases which is involved in junctional complexes assembly through the regulation of cell adhesion molecules transport to the plasma membrane and actin cytoskeleton reorganization. Regulates the endocytic recycling of occludins, claudins and E-cadherin to the plasma membrane and may thereby regulate the establishment of tight junctions and adherens junctions. In parallel, may regulate actin cytoskeleton reorganization directly through interaction with F-actin or indirectly through actinins and filamins. Most probably involved in the processes of epithelial cell differentiation, cell spreading and neurite outgrowth ().
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Research Articles on MILK2

1. Data indicate that MICAL-L2 may be an important regulator of epithelial-mesenchymal transition (EMT) in ovarian cancer cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-MILK2 antibody

 Products by Pathway  Pathway Diagram
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 Tight Junction Pathway antibodies  Tight Junction Pathway Diagram
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