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MMACHC Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1758391

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MMACHC, Polyclonal Antibody
Full Product Name
Anti-MMACHC Antibody Picoband
Product Gene Name
anti-MMACHC antibody
Product Synonym Names
group specific component ; Vitamin D-binding protein; DBP; VDB; Gc-globulin; Group-specific component; Gc
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
Molecular Weight
31,728 Da
OMIM
277400
3D Structure
ModBase 3D Structure for Q9Y4U1
Immunogen
E coli-derived human MMACHC recombinant protein (Position: M1-K242).
Subcellular Localization
Secreted
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross-reactivity with other proteins.
Protein Function
Involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation.

Product Categories/Family for anti-MMACHC antibody

Primary Antibodies; Actin Binding Proteins; Actin, etc.; Cancer; Cytoskeleton; Cytoskeleton/ECM; Microfilaments; Signal Transduction; Tumor Antigens; Tumor Biomarkers; Tumor Immunology; Tumor-Associated Antigens
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-MMACHC antibody
Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-MMACHC antibody
WB: 0.25-0.5ug/ml, Human
FC/FACS (Fixed): 1-3ug/1x106 cells, Human
ELISA: 0.1-0.5ug/ml

Western Blot (WB) of anti-MMACHC antibody

Figure 1. Western blot analysis of MMACHC using anti-MMACHC antibody (MBS1758391).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMACHC antigen affinity purified polyclonal antibody (#MBS1758391) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for MMACHC at approximately 32 kDa. The expected band size for MMACHC is at 32 kDa.
Rabbit Anti-MMACHC Polyclonal Antibody – Cat# MBS1758391

Flow Cytometry (FC/FACS) of anti-MMACHC antibody

Figure 2. Flow Cytometry analysis of JK cells using anti-MMACHC antibody (MBS1758391).
Overlay histogram showing JK cells stained with MBS1758391 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MMACHC Antibody (MBS1758391, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (MBS177405, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Rabbit Anti-MMACHC Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1758391

Product References and Citations for anti-MMACHC antibody

1. Ben-Omran, T. I., Wong, H., Blaser, S., Feigenbaum, A. Late-onset cobalamin-C disorder: a challenging diagnosis. Am. J. Med. Genet. 143A: 979-984, 2007.2. Bodamer, O. A. F., Rosenblatt, D. S., Appel, S. H., Beaudet, A. L. Adult-onset combined methylmalonic aciduria and homocystinuria (cblC). Neurology 56: 1113 only, 2001. 3. Collison, F. T., Xie, Y., Gambin, T., Jhangiani, S., Muzny, D., Gibbs, R., Lupski, J. R., Fishman, G. A., Allikmets, R. Whole exome sequencing identifies an adult-onset case of methylmalonic aciduria and homocystinuria type C (clbC) with non-syndromic bull's eye maculopathy. Ophthal. Genet. 36: 270-275, 2015.

Other Notes

Small volumes of anti-MMACHC antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MMACHC purchase

MBS1758391 is an antibody targeting MMACHC. Also known as Anti-MMACHC Antibody Picoband. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MMACHC. It may not necessarily be applicable to this product.

NCBI GI #
153070822
NCBI GeneID
25974
NCBI Accession #
NP_056321.2
UniProt Primary Accession #
Q9Y4U1
UniProt Secondary Accession #
Q5T157; Q9BRQ7
UniProt Related Accession #
Q9Y4U1
GenBank Nucleotide #
NM_015506.2
NCBI Official Full Name
methylmalonic aciduria and homocystinuria type C protein
NCBI Official Synonym Full Names
methylmalonic aciduria (cobalamin deficiency) cblC type, with homocystinuria
NCBI Official Symbol
MMACHC
NCBI Official Synonym Symbols
cblC
UniProt Protein Name
Methylmalonic aciduria and homocystinuria type C protein
Protein Family
Methylmalonic aciduria and homocystinuria type C protein
UniProt Gene Name
MMACHC
UniProt Entry Name
MMAC_HUMAN
NCBI Protein Information
methylmalonic aciduria and homocystinuria type C protein

NCBI Summary for MMACHC

The exact function of the protein encoded by this gene is not known, however, its C-terminal region shows similarity to TonB, a bacterial protein involved in energy transduction for cobalamin (vitamin B12) uptake. Hence, it is postulated that this protein may have a role in the binding and intracellular trafficking of cobalamin. Mutations in this gene are associated with methylmalonic aciduria and homocystinuria type cblC. [provided by RefSeq, Oct 2009]
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UniProt Comments for MMACHC

MMACHC: May be involved in the binding and intracellular trafficking of cobalamin (vitamin B12). Defects in MMACHC are the cause of methylmalonic aciduria and homocystinuria type cblC (MMAHCC). A disorder of cobalamin metabolism characterized by decreased levels of the coenzymes adenosylcobalamin (AdoCbl) and methylcobalamin (MeCbl). Affected individuals may have developmental, hematologic, neurologic, metabolic, ophthalmologic, and dermatologic clinical findings. Although considered a disease of infancy or childhood, some individuals develop symptoms in adulthood. Belongs to the MMACHC family.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1p34.1

Cellular Component: mitochondrion; cytosol

Molecular Function: protein binding; cobalamin binding

Biological Process: vitamin metabolic process; cobalamin metabolic process; cobalamin biosynthetic process; water-soluble vitamin metabolic process

Disease: Methylmalonic Aciduria And Homocystinuria, Cblc Type
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Research Articles on MMACHC

1. HCFC1 plays a role in craniofacial development, which is in part mediated through the regulation of MMACHC expression

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MMACHC antibody

 Reference Product  PubMed Publications
 MUT antibody  >5 publications with MMACHC and MUT
 MMADHC antibody  >5 publications with MMACHC and MMADHC
 MMAA antibody  >4 publications with MMACHC and MMAA
 MTR antibody  >3 publications with MMACHC and MTR
 MMAB antibody  >2 publications with MMACHC and MMAB
 CBS antibody  >1 publications with MMACHC and CBS

Pathways associated with anti-MMACHC antibody

 Products by Pathway  Pathway Diagram
 Cobalamin (Cbl, Vitamin B12) Transport And Metabolism Pathway antibodies  Cobalamin (Cbl, Vitamin B12) Transport And Metabolism Pathway Diagram
 Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway antibodies  Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway Diagram
 Defective BTD Causes Biotidinase Deficiency Pathway antibodies  Defective BTD Causes Biotidinase Deficiency Pathway Diagram
 Defective CD320 Causes Methylmalonic Aciduria Pathway antibodies  Defective CD320 Causes Methylmalonic Aciduria Pathway Diagram
 Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway antibodies  Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway Diagram
 Defective GIF Causes Intrinsic Factor Deficiency Pathway antibodies  Defective GIF Causes Intrinsic Factor Deficiency Pathway Diagram
 Defective HLCS Causes Multiple Carboxylase Deficiency Pathway antibodies  Defective HLCS Causes Multiple Carboxylase Deficiency Pathway Diagram
 Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway antibodies  Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway Diagram
 Defective MMAA Causes Methylmalonic Aciduria Type CblA Pathway antibodies  Defective MMAA Causes Methylmalonic Aciduria Type CblA Pathway Diagram
 Defective MMAB Causes Methylmalonic Aciduria Type CblB Pathway antibodies  Defective MMAB Causes Methylmalonic Aciduria Type CblB Pathway Diagram

Diseases associated with anti-MMACHC antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >39 publications with MMACHC and Nervous System Diseases
 Brain Diseases Antibodies  >37 publications with MMACHC and Brain Diseases
 Methylmalonic acidemia with homocystinuria Antibodies  >15 publications with MMACHC and Methylmalonic acidemia with homocystinuria
 Anemia Antibodies  >13 publications with MMACHC and Anemia
 Atrophy Antibodies  >7 publications with MMACHC and Atrophy
 Heart Defects, Congenital Antibodies  >5 publications with MMACHC and Heart Defects, Congenital
 Kidney Diseases Antibodies  >5 publications with MMACHC and Kidney Diseases
 Congenital Abnormalities Antibodies  >4 publications with MMACHC and Congenital Abnormalities
 Heart Diseases Antibodies  >3 publications with MMACHC and Heart Diseases
 Dementia Antibodies  >2 publications with MMACHC and Dementia

Organs/Tissues associated with anti-MMACHC antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >8 publications with MMACHC and Brain
 Eye Antibodies  >5 publications with MMACHC and Eye
 Kidney Antibodies  >4 publications with MMACHC and Kidney
 Skin Antibodies  >2 publications with MMACHC and Skin
 Bone Antibodies  >2 publications with MMACHC and Bone
 Liver Antibodies  >1 publications with MMACHC and Liver
 Lung Antibodies  >1 publications with MMACHC and Lung
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