Rabbit Anti-MMP20 Polyclonal Antibody – Cat# MBS1758678

Figure 1. Western blot analysis of MMP20 using anti-MMP20 antibody (MBS1758678).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Caco-2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP20 antigen affinity purified polyclonal antibody (#MBS1758678) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for MMP20 at approximately 54 kDa. The expected band size for MMP20 is at 54 kDa.

MMP20 Antibody – Rabbit Polyclonal

Name: MMP20 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1758678
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1758678

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring… More details

$210 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

MMP20, Polyclonal Antibody

Full Product Name

Anti-MMP20 Antibody Picoband

Product Gene Name

anti-MMP20 antibody

Product Synonym Names

Serine/threonine-protein kinase AtPK1/AtPK6; 70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70(S6K) alpha antibody, p70(S6K)-alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purity/Purification

Immunogen affinity purified.

Specificity

Expressed in all tissues.

Preparation and Storage

At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.

Molecular Weight

54,387 Da

3D Structure

ModBase 3D Structure for O60882

Immunogen

E coli-derived human MMP20 recombinant protein (Position: Y124-K405). Human MMP20 shares 91.5% amino acid (aa) sequence identity with mouse MMP20.

Subcellular Localization

Cytoplasm, Nucleus

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.

Cross Reactivity

No cross reactivity with other proteins.

Protein Function

Downstream effector of TOR signaling pathway involved in osmotic stress response. Could be involved in the control of plant growth and development. Phosphorylates the ribosomal proteins P14, P16 and S6. Functions as a repressor of cell proliferation and required for maintenance of chromosome stability and ploidy levels through the RBR1-E2F pathway.

Product Categories/Family for anti-MMP20 antibody

Primary Antibodies; Signal Transduction; Signaling Pathway; Small G Proteins

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-MMP20 antibody

Western Blot (WB), ELISA (EIA)

Application Notes for anti-MMP20 antibody

WB: 0.25-0.5ug/ml, Human
ELISA: 0.1-0.5ug/ml

Product References and Citations for anti-MMP20 antibody

1. Bartlett, J. D., Beniash, E., Lee, D. H., Smith, C. E. Decreased mineral content in MMP-20 null mouse enamel is prominent during the maturation stage. J. Dent. Res. 83: 909-913, 2004. 2. Caterina, J. J., Skobe, Z., Shi, J., Ding, Y., Simmer, J. P., Birkedal-Hansen, H., Bartlett, J. D. Enamelysin (matrix metalloproteinase 20)-deficient mice display an amelogenesis imperfecta phenotype. J. Biol. Chem. 277: 49598-49604, 2002. 3. Kim, J.-W., Simmer, J. P., Hart, T. C., Hart, P. S., Ramaswami, M. D., Bartlett, J. D., Hu, J. C.-C. MMP-20 mutation in autosomal recessive pigmented hypomaturation amelogenesis imperfecta. J. Med. Genet. 42: 271-275, 2005.

Other Notes

Small volumes of anti-MMP20 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MMP20 purchase

MBS1758678 is an antibody targeting MMP20. Also known as Anti-MMP20 Antibody Picoband. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MMP20. It may not necessarily be applicable to this product.

NCBI GI #

45359865

NCBI GeneID

9313

NCBI Accession #

NP_004762.2

UniProt Primary Accession #

O60882

UniProt Secondary Accession #

Q6DKT9

UniProt Related Accession #

O60882

GenBank Nucleotide #

NM_004771.3

NCBI Official Full Name

matrix metalloproteinase-20 preproprotein

NCBI Official Synonym Full Names

matrix metallopeptidase 20

NCBI Official Symbol

MMP20

NCBI Official Synonym Symbols

AI2A2; MMP-20

UniProt Protein Name

Matrix metalloproteinase-20

UniProt Synonym Protein Names

Enamel metalloproteinase; Enamelysin

Protein Family

Matrix metalloproteinase

UniProt Gene Name

MMP20

UniProt Synonym Gene Names

MMP-20

NCBI Protein Information

matrix metalloproteinase-20

NCBI Summary for MMP20

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene degrades amelogenin, the major protein component of dental enamel matrix, and thus thought to play a role in tooth enamel formation. A mutation in this gene, which alters the normal splice pattern and results in premature termination of the encoded protein, has been associated with amelogenesis imperfecta. This gene is part of a cluster of MMP genes located on chromosome 11q22.3. [provided by RefSeq, Aug 2011]

UniProt Comments for MMP20

Degrades amelogenin, the major protein component of the enamel matrix and two of the macromolecules characterizing the cartilage extracellular matrix: aggrecan and the cartilage oligomeric matrix protein (COMP). May play a central role in tooth enamel formation. Cleaves aggrecan at the '360-Asn-|-Phe-361' site.

Research Articles on MMP20

1. common variants at 11q22.2 within MMP20 associate with neuroblastoma cases harboring 11q deletion (rs10895322)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-MMP20 antibody

Products by Pathway	Pathway Diagram
Matrix Metalloproteinases Pathway antibodies	Matrix Metalloproteinases Pathway Diagram

Diseases associated with anti-MMP20 antibody

Disease Name	Pubmed Publications
Growth Disorders Antibodies	>2 publications with MMP20 and Growth Disorders
Fibrosis Antibodies	>2 publications with MMP20 and Fibrosis
Disease Models, Animal Antibodies	>2 publications with MMP20 and Disease Models, Animal

Organs/Tissues associated with anti-MMP20 antibody

Organ/Tissue Name	Pubmed Publications
Connective Tissue Antibodies	>1 publications with MMP20 and Connective Tissue