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MSH2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8110488

MSH2 is a key DNA mismatch repair protein, which plays an important role in genomic stability. In addition to its DNA repair function, MSH2 serves as a sensor for DNA base analogs-provoked DNA replication errors and binds to various DNA damage-induced adducts… More details

$370 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MSH2, Polyclonal Antibody
Full Product Name
Anti-MSH2 Antibody, Rabbit Polyclonal
Product Gene Name
anti-MSH2 antibody
Product Synonym Names
MSH2 Antibody, Rabbit PAb, Antigen Affinity Purified; mutS homolog 2; Anti-COCA1 Antibody; Anti-FCC1 Antibody; Anti-HNPCC Antibody; Anti-HNPCC1 Antibody; Anti-LCFS2 Antibody
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Purity/Purification
Protein A & Antigen Affinity
Specificity
Human MSH2
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Immunogen
E Coli-derived Human MSH2 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human MSH2 fragment, and purified by antigen affinity chromatography.

Product Categories/Family for anti-MSH2 antibody

Tumor Suppressors
Applications Tested/Suitable for anti-MSH2 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-MSH2 antibody
WB: 1:500-1:2000
IP: 1-5uL/mg of lysate

Western Blot (WB) of anti-MSH2 antibody

Anti-MSH2 rabbit polyclonal antibody at 1:500 dilution
Lane A: Hela Whole Cell Lysate
Lane B: A549 Whole Cell Lysate
Lysates/proteins at 30 ug per lane.
Secondary
Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:105 kDa
Observed band size:95 kDa
(We are unsure as to the identity of these extra bands.)
Rabbit Anti-MSH2 Polyclonal Antibody – Cat# MBS8110488

Immunoprecipitation (IP) of anti-MSH2 antibody

IVD044 was immunoprecipitated using:
Lane A:0.5 mg Hela Whole Cell Lysate
4 uL anti-IVD044 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.
Primary antibody:
Anti-IVD044 rabbit polyclonal antibody,at 1:100 dilution
Secondary antibody:
Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 95 kDa
Observed band size :95 kDa
Rabbit Anti-MSH2 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS8110488

Other Notes

Small volumes of anti-MSH2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MSH2 purchase

MBS8110488 is an antibody targeting MSH2. Also known as Anti-MSH2 Antibody, Rabbit Polyclonal. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MSH2. It may not necessarily be applicable to this product.

Protein Family
DNA mismatch repair protein

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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