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MYL9 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6323368

MBS6323368 is an antibody targeting MYL9. Also known as MYL9, CT (MYL9, MLC2, MRLC1, MYRL2, Myosin regulatory light polypeptide 9, 20kD myosin light chain, MLC-2C, Myosin RLC, Myosin regulatory light chain 2, smooth muscle isoform, Myosin regulatory light… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MYL9, Polyclonal Antibody
Full Product Name
MYL9, CT (MYL9, MLC2, MRLC1, MYRL2, Myosin regulatory light polypeptide 9, 20kD myosin light chain, MLC-2C, Myosin RLC, Myosin regulatory light chain 2, smooth muscle isoform, Myosin regulatory light chain 9, Myosin regulatory light chain MRLC1) (MaxLight
Product Gene Name
Product Synonym Names
MYL9; MLC2; MRLC1; MYRL2; Myosin regulatory light polypeptide 9; 20kD myosin light chain; MLC-2C; Myosin RLC; Myosin regulatory light chain 2; smooth muscle isoform; Myosin regulatory light chain 9; Myosin regulatory light chain MRLC1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.
Purity/Purification
Purified by Protein A and Peptide Affinity Chromatography.
Specificity
Recognizes human MYL9.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
13,866 Da
OMIM
609905
3D Structure
ModBase 3D Structure for P24844
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 96-125 in the C-terminal region of human MYL9, UniProt Accession #P24844. Species sequence homology: chicken, mouse, porcine, rat
Conjugate
MaxLight405
Note
Preservative Free

Product Categories/Family for anti-MYL9 antibody

Applications Tested/Suitable for anti-MYL9 antibody
Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB)
Application Notes for anti-MYL9 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-MYL9 antibody

Western Blot analysis in Jurkat cell line lysates (35ug/lane) usingMBS6004914. This demonstrates that MBS6004914 detected the MYL9 protein (arrow).
Rabbit Anti-MYL9 Polyclonal Antibody – Cat# MBS6323368

Immunohistochemistry (IHC) of anti-MYL9 antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue using MBS6004914 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6004914 for immunohistochemistry.
Rabbit Anti-MYL9 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6323368

Product References and Citations for anti-MYL9 antibody

1. Gilles, L., et al. Blood 114(19):4221-4232(2009). 2. Higashihara, M., et al. J Smooth Muscle Res 44(1):29-40(2008). 3. Szczesna-Cordary, D., et al. J. Cell. Sci. 118 (PT 16), 3675-3683 (2005). 4. Webb, R.C. Adv Physiol Educ 27 (1-4), 201-206 (2003). 5. Deloukas, P., et al. Nature 414(6866):865-871(2001).

Other Notes

Small volumes of anti-MYL9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MYL9. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
myosin regulatory light polypeptide 9 isoform a
NCBI Official Synonym Full Names
myosin, light chain 9, regulatory
NCBI Official Symbol
MYL9
NCBI Official Synonym Symbols
LC20; MLC2; MRLC1; MYRL2; MLC-2C
UniProt Protein Name
Myosin regulatory light polypeptide 9
UniProt Synonym Protein Names
20 kDa myosin light chain; LC20; MLC-2C; Myosin RLC; Myosin regulatory light chain 2, smooth muscle isoform; Myosin regulatory light chain 9; Myosin regulatory light chain MRLC1
UniProt Gene Name
MYL9
UniProt Synonym Gene Names
MLC2; MRLC1; MYRL2; LC20
UniProt Entry Name
MYL9_HUMAN
NCBI Protein Information
myosin regulatory light polypeptide 9; 20 kDa myosin light chain; myosin RLC; myosin regulatory light chain 1; myosin regulatory light chain 2, smooth muscle isoform; myosin regulatory light chain 9; myosin regulatory light chain MRLC1; myosin, light poly

NCBI Summary for MYL9

Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for MYL9

MRLC1: Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion. Myosin is an hexamer of 2 heavy chains and 4 light chains. Smooth muscle tissues and in some, but not all, nonmuscle cells. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Contractile

Chromosomal Location of Human Ortholog: 20q11.23

Cellular Component: stress fiber; muscle myosin complex; cytosol; Z disc

Molecular Function: structural constituent of muscle; calcium ion binding

Biological Process: axon guidance; muscle contraction; regulation of muscle contraction; ephrin receptor signaling pathway
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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