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ND5 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6006325

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The… More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ND5, Polyclonal Antibody
Full Product Name
ND5, CT (NADH-ubiquinone Oxidoreductase Chain 5, NADH Dehydrogenase Subunit 5, NADH5, Mitochondrially Encoded NADH Dehydrogenase 5, MTND5, MT-ND5)
Product Synonym Names
Anti -ND5, CT (NADH-ubiquinone Oxidoreductase Chain 5, NADH Dehydrogenase Subunit 5, NADH5, Mitochondrially Encoded NADH Dehydrogenase 5, MTND5, MT-ND5)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
Recognizes human ND5.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide selected from the C-terminal region of human ND5 (KLH).
Applications Tested/Suitable for anti-ND5 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-ND5 antibody
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500

Western Blot (WB) of anti-ND5 antibody

Western Blot analysis in CEM cell line lysates (35ug/lane) using MBS6006325. ND5 (arrow) was detected using MBS6006325.
Rabbit Anti-ND5 Polyclonal Antibody – Cat# MBS6006325

Testing Data of anti-ND5 antibody

All lanes: MBS6006325 (1:1000) Lane 1: CCRF-CEM whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 67kD. Blocking/Dilution buffer: 5% NFDM/TBST.
Rabbit Anti-ND5 Polyclonal Antibody, Testing Data – Cat# MBS6006325

Immunohistochemistry (IHC) of anti-ND5 antibody

Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human brain tissue reacted with MBS6006325, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS6006325 for immunohistochemistry.
Rabbit Anti-ND5 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6006325

Other Notes

Small volumes of anti-ND5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ND5 purchase

MBS6006325 is an antibody targeting ND5. Also known as ND5, CT (NADH-ubiquinone Oxidoreductase Chain 5, NADH Dehydrogenase Subunit 5, NADH5, Mitochondrially Encoded NADH Dehydrogenase 5, MTND5, MT-ND5). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ND5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
NCBI Official Full Name
ND5 (mitochondrion)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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